Search Results - "Jichu yixue yu linchuang"

Objective To investigate the role of a novel human-specific long noncoding RNA (lncRNA), MEK6-AS1, and its potential molecular mechanism in improving the osteogenic differentiation of senescent mesenchymal stem cells (MSCs). Methods An in vitro human MSCs replicative senescence model was established...

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Objective To investigate the role of long non-coding RNA-small nucleolar RNA host gene 3(lncRNA-Snhg3) and its regulatory mechanism in the hepatic glucose metabolism of mice. Methods Adenovirus Snhg3 was over-expressed by the tail vein injection in db/db mice, and then glucose tolerance and pyruvate...

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Obstructive sleep apnea (OSA) is a multifactor respiratory disorder characterized by recurrent episodes of airway collapse, leading to intermittent hypoxemia, hypercapnia, and sleep fragmentation. Intermittent hypoxia (IH) has profound effects on various body systems, particularly the immune system....

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Objective To investigate the effect of mutation human proprotein convertase subtilism/kexin type 9(hPCSK9D374Y) in PCSK9 gene on methionine choline deficiency diet (MCD)-induced nonalcoholic steato-hepatitis (NASH) in mice. Methods Sixteen C57BL/6J wild-type mice were selected and randomly divided i...

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Objective To construct an in vitro research model for studying human liver regeneration based on human pluripotent stem cells (hPSCs)-derived hepatocyte-like organoid (HLO). Methods The hPSCs-derived HLO was obtained by inducing differentiation and the regeneration model after liver injury was const...

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Objective To screen the active chemical components with potential therapeutic effects against lung cancer in tea and to provide new insights into the treatment and prevention of lung cancer. Methods Based on network pharmacology, the main active components from 13 types of tea samples were analyzed...

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Objective To identify the impact of glucose variability (GV) on the development of gestational hypertension (GH) in patients with gestational diabetes mellitus (GDM), and to find the differences between various GV metrics as well as to evaluate their predictive value in management strategy developme...

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Objective To investigate the function of the glutamic-oxaloacetic transaminase 2(GOT2) gene in acute myeloid leukemia cells. Methods ShRNAs targeting at GOT2 were constructed to suppress GOT2 expression in the THP-1 human monocytic leukemia cell line. GOT2 mRNA level was measured by RT-qPCR, and GOT...

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Objective To investigate the role of homocysteine (Hcy) in the pathogenesis of irritable bowel syndrome (IBS) and its effects on intestinal motility, visceral hypersensitivity, and barrier function. Methods Clinical cohorts and animal models were combined for this study. Clinically, fifteen IBS pati...

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Objective To find specific mechanisms through which immune cell activation affects central nervous system inflammation in Alzheimer′s disease (Alzheimer′s disease, AD). Methods The public single-cell human brain database GSE161045 was applied for analysis. Then bulk RNA sequencing was performed in 5...

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Objective To explore the application of case-based learning(CBL) approach in the basic medical stage for eight-year clinical medical program. Methods A total of 320 students from the eight-year clinical medicine program at Peking Union Medical College in the grades of 2014—2015 and 2019—2020 were se...

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Objective To construct fluorescent molecular probes targeting at tumor heat shock protein 90 (Hsp90) in order to enhance tumor-specific recognition. Methods The human pancreatic adenocarcinoma cell line PANC-1 and the human small cell lung cancer cell line NCI-H446 were used as the research targets...

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Objective To explore the association between disease activity and health utility values(HUV) in patients with rheumatoid arthritis(RA) in China, and to analyze other factors that may affect health utility values, providing a basis for individualized treatment and health management for RA patients. M...

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Objective To detect the expression of cytochrome c oxidase assembly factor 6(COA6) in breast cancer, study its clinical significance, and analyze the effect of COA6 on immune infiltration in breast cancer. Methods Differential genes were screened by the whole transcriptome sequencing and the express...

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Objective To identify potential serum metabolic biomarkers in colorectal cancer (CRC) patients using untargeted metabolomics and to evaluate their diagnostic and staging value. Methods Serum samples from 100 healthy controls and 100 CRC patients were analyzed by ultra-performance liquid chromatograp...

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Objective To develop a modified lentiviral expression system of mCherry-GFP-LC3B, driven by the hematopoietic-specific SFFV (spleen focus-forming virus) promoter, in order to perform an efficient and real-time monitoring of autophagy flux with in terminally differentiated erythroid cells. Methods Th...

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Objective Compared with endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA), this study aims to explore the diagnostic advantages of endobronchial ultrasound-guided transbronchial node biopsy (EBUS-TBNB) in the diagnosis of mediastinal lymph node metastasis, lymphadenitis, s...

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Objective To comprehensively understand the map of transcripts during mitosis and their regulatory mechanisms of HAP1 cells by conducting transcriptome sequencing analysis after being released by mitotic synchronization arrest. Methods HAP1 cells were subjected to mitotic synchronous arrest with noc...

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Objective To investigate the transcriptional and translational expression of Slitrk6 in rat spermatogenesis. Methods The mRNA transcription level and translation level of Slitrk6 were detected by RT-qPCR and Western blot, respectively in testis of rats aged 2-65 days (repeat three times at each time...

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Objective To explore the role of pseudogene AC106872.1 in maintaining the self-renewal capacity of human embryonic stem cells (hESCs). Methods AC106872.1 was knocked out in hESCs and knockout efficiency was validated by PCR and agarose gel electrophoresis. The colony formation of hESCs was assessed...

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