Search Results - "ELISA"
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Development and detection application of monoclonal antibodies against Zucchini yellow mosaic virus
Published 2017-01-01Subjects: Get full textAphid-borne Zucchini yellow mosaic virus (ZYMV) is one of the most economically important viruses of cucurbitaceous plants. To survey and control this virus, it is necessary to develop an efficient detection technique. Using purified ZYMV virion and the conventional hybridoma technology, three hybri...
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Heat Shock Protein70 as Biomarker in Bladder Cancer
Published 2014-09-01Subjects: “…UBC, HSP, ELISA…”The present study aimed to shed light on the urine HSP70 concentration of patients with urinary bladder carcinoma UBC and control subjects as new urinary biomarker. The second aim was to associate this protein concentration with UBC stage and grade in patients with UBC. A direct ELISA was used to...
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Preparation and detection application of monoclonal antibodies against Potato virus Y
Published 2016-09-01Subjects: Get full textPotato virus Y (PVY) is one of the most important potato viruses causing serious economic loss in potato industry worldwide. Establishment of a sensitive and specific virus detection technique is the key to prevent and control PVY.To provide material and technical support for the diagnosis, scientif...
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Monoclonal antibody-based serological detection of potato virus M in potato plants and tubers
Published 2020-05-01Subjects: Get full textPotato virus M (PVM) is one of the common and economically important potato viruses in potato-growing regions worldwide. To investigate and control this viral disease, efficient and specific detection techniques are needed. In this study, PVM virions were purified from infected potato plants and use...
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Preparation and validation of anti-chlortetracycline polyclonal antibodies
Published 2008-03-01Subjects: Get full textAnti-chlortetracycline (CTC) polyclonal antibodies for effective residue detection were produced and studied. Two kinds of CTC immunogens were prepared via glutaraldehyde and mixed acid anhydride methods. New Zealand rabbits were immunized with these immunogens to produce the polyclonal antibodies,...
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Sero-epidemiological Study of Toxoplasma gondii among Pregnant Women in Akre City
Published 2020-12-01Subjects: “…Toxoplasma gondii , Toxoplasmosis, ELISA ,…”Recently has been observed globally that toxoplasmosis disease was caused by Toxoplasma gondii and generally its asymptomatic infection in people except pregnant women. Many previous studies were suggested this pathogen transmits by ingesting of undercooked or raw meat contaminated with the oocytes...
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MANUFACTURING OF HYBRIDOMAS-PRODUCERS OF MONOCLONAL ANTIBODIES TO BRUCELLOSIS AGENT ANTIGENS
Published 2017-06-01Subjects: Get full textObjective of study is to prepare hybridomas-producers of monoclonal antibodies to brucellosis agent antigens. Materials and methods. B. abortus, B. melitensis, B. suis strains from the State collection of microorganisms of the 48th Central Research Institute Affiliated Branch and BALB/c mice. Hybrid...
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Development and Validation of the ELISA Method for Anti-trastuzumab Antibodies Determination in Human Serum
Published 2022-12-01Subjects: Get full textIntroduction. One of the widely used specific anti-HER2 (human epidermal growth factor receptor 2) MAb drugs is trastuzumab. Trastuzumab is highly effective for malignant HER2 hyperexpression reduction, which results in HER2 oncogenicity decrease. As any other biotherapeutics trastuzumab can cause i...
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Validation of ELISA Test-system for Trastuzumab (Herceptin, Hertikad) quantitative determination in biological fluids
Published 2023-05-01Subjects: Get full textRelevance. Trastuzumab is the drug of choice for the HER2+ breast cancer treatment. To determine the trastuzumab pharmacodynamics in personalized therapy a validated bioanalytical method for measuring the concentration of the drug in biological fluids is required. The aim: creation and assessment of...
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Study on the monoclonal antibody to chlorpyrifos
Published 2006-11-01Subjects: Get full textOne hybridoma cell line (1G10) secreting monoclonal antibody (McAb) against chlorpyrifos was produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/C which were immunized by the artificial antigen conjugated with bovine serum albumin (BSA). Isotype and subclass of the monoclonal...
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Three sensitive and reliable serological assays for detection of potato virus A in potato plants
Published 2021-11-01Subjects: Get full textVegetative propagation of seed potato often allows passaging of viruses to seed tubers, resulting in significant yield losses and reduction of potato tuber quality. Thus, virus detection approach is crucial for effective virus management programs and the production of virus-free seed potatoes. Among...
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Development of a Broad-Spectrum Antigen-Capture ELISA Using Combined Anti-p26 Polyclonal and Monoclonal Antibodies for Detection of Equine Infectious Anemia Virus
Published 2025-06-01Subjects: Get full textEquine Infectious Anemia Virus (EIAV) poses significant diagnostic challenges due to its genetic variability and the limitations of conventional nucleic acid detection methods. This study developed an antigen-capture, enzyme-linked immunosorbent assay (AC-ELISA) for the detection and quantification...
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EXPRESSION LEVELS OF SOME ADHESION MOLECULES IN THE INTACT AND UV-IRRADIATED Т-LYMPHOCYTES FROM HUMAN BLOOD
Published 2014-07-01Subjects: Get full textAbstract. While employing an enzyme linked immunosorbent assay, it was shown that UV-sensitivity is different for various adhesion molecules (CD2, CD11a and CD29) at the membranes of T-lymphocytes. Relative photoresistance of CD2 and CD11a antigens to UV irradiation was established at the doses rang...
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Evaluation the Efficacy of ELISA IgG, IgM and IgA Tests for Diagnosis of Helicobacter pylori
Published 2018-09-01Subjects: “…ELISA, Antibody, Evaluation, H. pylori…”It has been well recognized throughout the world that Helicobacter pylori is the main cause of gastric ulcer and stomach carcinoma. Laboratory diagnosis of H. pylori infection is made by invasive and non-invasive methods. Invasive methods require endoscopy which is uncomfortable an unacceptable by...
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Quantification of lysozyme in hyperimune egg white aquous extracts and in biological products by SANDWICH ELISA test
Published 2020-06-01Subjects: Get full textLysozyme is a biologically active protein present in egg whites, having an essential role in the immune defense of the embryo. Due to its antimicrobial properties, it is extracted and used in the composition of various products as a preservative and/or a bioactive ingredient. Its qualitative and qua...
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Serological Diagnosis of Respiratory Syncytial Virus by ELISA Technique among Children with Respiratory Tract Infections in Beiji City, Iraq
Published 2023-02-01Subjects: Get full textThe study have been done to detect Human Respiratory syncytial virus (HRSV) infections among hospitalized children under 5 years old in Beiji city Salah-Aldin Governorate, in addition to identify the risk factors associated with the virus. Whole blood samples were taken from 300 hospitalized childr...
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Validation of an enzyme-linked immunosorbent assay test-system for bevacizumab concentration determing in biological fluids
Published 2024-06-01Subjects: Get full textIntroduction. One of the reasons for the development of many diseases primarily malignant is the increased expression of Vascular Endothelial Growth Factor (VEGF). Bevacizumab is a drug that neutralizes the biological activity of VEGF. The molecular structure of bevacizumab is a recombinant humanize...
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A Double-blind Randomized Comparative Phase I Study to Assess Biosimilarity and Immunogenicity of "Trastuzumab" (LLC "Mabscale", Russia) and Herceptin® (F. Hoffmann-La Roche Ltd.,...
Published 2023-09-01Subjects: Get full textIntroduction. Trastuzumab is the first drug based on the monoclonal antibodies’ technology targeted to the neu oncogene expression product discovered in the middle 80-s – human epidermal growth receptor, HER2. After being approved trastuzumab had become the drug of choice for combine therapy of meta...
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ELISA method in identification of the blood in the stains on the material evidences
Published 2013-09-01Subjects: Get full textThe article presents the analysis of experience, both of domestic, and of foreign authors in application of the techniques for identification of the blood in the stains on material evidences. The method of definition of IgG by means of ELISA is compared with other modern techniques applied in Russia...
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Evaluate the efficiency of Widal test in diagnosis of typhoid Fever in arriving patients to Kirkuk hospitals
Published 2023-02-01Subjects: Get full textThe aim of this study was to investigate the accuracy of widal test by compared with enzyme linked immunosorbent assay (ELISA) as standard method and for this purpose 150 blood samples were pulled from patients suspected infected with typhoid fever coming to Azadi Teaching Hospital and Children�...
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