Risultati della ricerca - "PCR detection"
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Development of the PCR Assay with Universal Primers for the Detection of Different <i>tcpA</i> Gene Variants
Pubblicazione 2012-02-01Soggetti: TestoDeveloped is the PCR assay for the detection of the structural genes of toxin co-regulated adhesion piluses - tcpA of different types. Determined are the universal primers, the usage of which provides for the detection of the stated above genes in V. cholerae of various serogroups. With the help of...
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Biological and molecular characterization of tomato brown rugose fruit virus and development of quadruplex RT-PCR detection
Pubblicazione 2021-07-01Soggetti: TestoTomato brown rugose fruit virus (ToBRFV) is a novel tobamovirus firstly reported in 2015 and poses a severe threat to the tomato industry. So far, it has spread to 10 countries in America, Asia, and Europe. In 2019, ToBRFV was identified in Shandong Province (ToBRFV-SD), China. In this study, it was...
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Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus
Pubblicazione 2013-02-01Soggetti: TestoSuppression subtractive hybridization (SSH) was performed with virulent strain ATCC35246 and avirulent strain ST171 to identify novel genes associated with virulence in Streptococcus equi ssp. zooepidemicus (SEZ). There were fourteen genomic regions that only presented in virulent strain ATCC35246....
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Transmission of potato spindle tuber viroid between <i>Phytophthora infestans</i> and host plants
Pubblicazione 2022-06-01Soggetti: TestoPotato spindle tuber viroid (PSTVd) is a naked, circular, single-stranded RNA (356–363 nucleotides in length) which lacks any protein-coding sequences. It is an economically important pathogen and is classified as a high-risk plant quarantine disease. Moreover, it is known that PSTVd is mechanically...
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A FIASCO-Based Approach for Detection and Diagnosis of Puccinia graminis f. sp. tritici in China
Pubblicazione 2014-11-01Soggetti: TestoStem or black rust of wheat, caused by the fungus Puccinia graminis Pers. f. sp. tritici Eriks. & E. Henn. (Pgt), has historically caused severe losses to wheat (Triticum aestivum L.) production worldwide. In the Fujian and Guangdong provinces of China, six moderate-to-severe epidemics of wh...
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