HPLC-MS analysis, antioxidant and hepatoprotective potential of okra fruit (Abelmoschus esculentus) against carbon tetrachloride induced hepatic damage
This study exploits the bioactive components of the species Abelmoschus esculentus and examines the polyphenolic extract's antioxidant and hepatoprotective potential. The phytochemical characterization was performed using High-Performance Liquid Chromatography-Mass Spectrometry analysis, follo...
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2025-06-01
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| Series: | Notulae Scientia Biologicae |
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| Online Access: | https://www.notulaebiologicae.ro/index.php/nsb/article/view/12312 |
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| author | Hamza BELKHODJA Fatima Z. LABBACI Abderrahmane REMIL Djilali BOUHADI Mohammed HARIR Fehmi BOUFAHJA |
| author_facet | Hamza BELKHODJA Fatima Z. LABBACI Abderrahmane REMIL Djilali BOUHADI Mohammed HARIR Fehmi BOUFAHJA |
| author_sort | Hamza BELKHODJA |
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This study exploits the bioactive components of the species Abelmoschus esculentus and examines the polyphenolic extract's antioxidant and hepatoprotective potential. The phytochemical characterization was performed using High-Performance Liquid Chromatography-Mass Spectrometry analysis, followed by the evaluation of the antioxidant activity in vitro and the study of the hepatoprotective potential against hepatic lesions induced by carbon tetrachloride in mice. Two main compounds were identified by HPLC-MS analysis: quercetin and kaempferol. The in vitro antioxidant activity of A. esculentus extracts showed interesting free-radical properties manifested by low IC50 values compared to ascorbic acid. The macerated aqueous extract of A. esculentus has an interesting hepatoprotective effect against the lesions induced by carbon tetrachloride in mice, as evidenced by weight gain, a normal hepato-somatic index, and improvement in biochemical parameters. A. esculentus is of considerable therapeutic interest as an alternative compound for preventing hepatic disorders and lesions.
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| format | Article |
| id | doaj-art-fd3618f5bae6475e91704b5f3db4f12d |
| institution | Matheson Library |
| issn | 2067-3264 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj-art-fd3618f5bae6475e91704b5f3db4f12d2025-07-02T20:14:06ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32642025-06-0117210.55779/nsb17212312HPLC-MS analysis, antioxidant and hepatoprotective potential of okra fruit (Abelmoschus esculentus) against carbon tetrachloride induced hepatic damageHamza BELKHODJA0Fatima Z. LABBACI1Abderrahmane REMIL2Djilali BOUHADI3Mohammed HARIR4Fehmi BOUFAHJA5University of Mustapha Stambouli, Laboratory of Bioconversion, Microbiology Engineering and Health Safety, Mascara, 29000University of Mustapha Stambouli, Laboratory of Chemistry and Physics of Macromolecules and Biological Interfaces, Mascara, 29000University of Mustapha Stambouli, Department of Biology, Mascara, 29000, University of Mustapha Stambouli, Laboratory of Bioconversion, Microbiology Engineering and Health Safety, Mascara, 29000University of Sciences and Technology Mohamed Boudiaf, Faculty of Natural and Life Sciences, Department of Applied Molecular Genetics, OranImam Mohammad Ibn Saud Islamic University (IMSIU), College of Science, Biology Department, Riyadh 11623, Saudi Arabia This study exploits the bioactive components of the species Abelmoschus esculentus and examines the polyphenolic extract's antioxidant and hepatoprotective potential. The phytochemical characterization was performed using High-Performance Liquid Chromatography-Mass Spectrometry analysis, followed by the evaluation of the antioxidant activity in vitro and the study of the hepatoprotective potential against hepatic lesions induced by carbon tetrachloride in mice. Two main compounds were identified by HPLC-MS analysis: quercetin and kaempferol. The in vitro antioxidant activity of A. esculentus extracts showed interesting free-radical properties manifested by low IC50 values compared to ascorbic acid. The macerated aqueous extract of A. esculentus has an interesting hepatoprotective effect against the lesions induced by carbon tetrachloride in mice, as evidenced by weight gain, a normal hepato-somatic index, and improvement in biochemical parameters. A. esculentus is of considerable therapeutic interest as an alternative compound for preventing hepatic disorders and lesions. https://www.notulaebiologicae.ro/index.php/nsb/article/view/12312Abelmoschus esculentuscarbon tetrachloridehepatic damageoxidative stress |
| spellingShingle | Hamza BELKHODJA Fatima Z. LABBACI Abderrahmane REMIL Djilali BOUHADI Mohammed HARIR Fehmi BOUFAHJA HPLC-MS analysis, antioxidant and hepatoprotective potential of okra fruit (Abelmoschus esculentus) against carbon tetrachloride induced hepatic damage Notulae Scientia Biologicae Abelmoschus esculentus carbon tetrachloride hepatic damage oxidative stress |
| title | HPLC-MS analysis, antioxidant and hepatoprotective potential of okra fruit (Abelmoschus esculentus) against carbon tetrachloride induced hepatic damage |
| title_full | HPLC-MS analysis, antioxidant and hepatoprotective potential of okra fruit (Abelmoschus esculentus) against carbon tetrachloride induced hepatic damage |
| title_fullStr | HPLC-MS analysis, antioxidant and hepatoprotective potential of okra fruit (Abelmoschus esculentus) against carbon tetrachloride induced hepatic damage |
| title_full_unstemmed | HPLC-MS analysis, antioxidant and hepatoprotective potential of okra fruit (Abelmoschus esculentus) against carbon tetrachloride induced hepatic damage |
| title_short | HPLC-MS analysis, antioxidant and hepatoprotective potential of okra fruit (Abelmoschus esculentus) against carbon tetrachloride induced hepatic damage |
| title_sort | hplc ms analysis antioxidant and hepatoprotective potential of okra fruit abelmoschus esculentus against carbon tetrachloride induced hepatic damage |
| topic | Abelmoschus esculentus carbon tetrachloride hepatic damage oxidative stress |
| url | https://www.notulaebiologicae.ro/index.php/nsb/article/view/12312 |
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