Impact of naringenin in inhibition of protein glycation in diabetic rats as a protection mechanism against nephropathy
Protein glycation is one of the most serious issues in diabetes playing a critical role in the development of many cellular dysfunction as aging, cardiovascular diseases and neural disorders. This study investigated the impact of naringenin on the glycation rate of glomerular basement membrane prot...
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PAGEPress Publications
2025-07-01
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| Series: | Journal of Biological Research |
| Online Access: | https://www.pagepressjournals.org/jbr/article/view/13600 |
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| author | Taha A. Kumosani Abeer Alnefayee Elie Barbour Mohammed Qari Tarek Ahmed Said S. Moselhy |
| author_facet | Taha A. Kumosani Abeer Alnefayee Elie Barbour Mohammed Qari Tarek Ahmed Said S. Moselhy |
| author_sort | Taha A. Kumosani |
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Protein glycation is one of the most serious issues in diabetes playing a critical role in the development of many cellular dysfunction as aging, cardiovascular diseases and neural disorders. This study investigated the impact of naringenin on the glycation rate of glomerular basement membrane protein in diabetic rats to prevent nephropathy as a complication of diabetes. Fifty male Wistar rats were sorted into 2 main groups, Gr I (normal; n=10) and Gr II (Diabetic, n=40). Diabetes was induced in rats by injection of a single dose of 60 mg/kg, i.p. streptozocin (STZ). Diabetic rats were allocated to Gr IIa: (not treated), Gr IIb: treated with naringenin (50 mg/kg body weight - bw), Gr IIc: Diabetic treated with naringenin (100 mg/kg bw), Gr IId: Diabetic treated with metformin (100 mg/kg bw). Serum was utilized for the determination of malondialdehyde (MDA), glycated hemoglobin (HA1c), fructosamine, and total antioxidant activity. Kidney tissue was utilized for the determination of oxidative stress and advanced glycated end products (AGEs). Data obtained showed that naringenin supplementation in diabetic rats reduced the levels of serum MDA (p=0.0111), HA1c (p=0.0112), fructosamine (p=0.011), and improved antioxidant capacity (p=0.032). In addition, it reduced the level of AGEs (p=0.0008), and enhanced antioxidant enzymes (p<0.01134) compared with untreated. Diabetic rats showed a significant elevation in inflammatory markers tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), AGEs, decreased reduced glutathione, Superoxide Dismutase (SOD), Catalase (CAT) activity in kidney tissue versus control. The effect of naringenin was dose dependent, and improved these alterations versus untreated (p=0.0111). It was concluded that the inhibition of the formation of AGEs by naringenin in diabetics contributed to the protection against complications. Therefore, naringenin and its mechanisms of action is promising for development of safety and effective new antidiabetic agent.
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| format | Article |
| id | doaj-art-fd1dced5a6854e2daa4cbc3c8cde72d0 |
| institution | Matheson Library |
| issn | 1826-8838 2284-0230 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | PAGEPress Publications |
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| series | Journal of Biological Research |
| spelling | doaj-art-fd1dced5a6854e2daa4cbc3c8cde72d02025-07-08T13:21:48ZengPAGEPress PublicationsJournal of Biological Research1826-88382284-02302025-07-019810.4081/jbr.2025.13600Impact of naringenin in inhibition of protein glycation in diabetic rats as a protection mechanism against nephropathyTaha A. Kumosani0Abeer Alnefayee1Elie Barbour2Mohammed Qari3Tarek Ahmed4Said S. Moselhy5Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; Production of Bio-products for Industrial Application Research Group, King Abdulaziz University, JeddahBiochemistry Department, Faculty of Science, King Abdulaziz University, JeddahExperimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; Production of Bio-products for Industrial Application Research Group, King Abdulaziz University, JeddahHematology Department, Faculty of Medicine, King Abdulaziz University, JeddahDepartment of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, CairoBiochemistry Department, Faculty of Science,Ain Shams University, Cairo Protein glycation is one of the most serious issues in diabetes playing a critical role in the development of many cellular dysfunction as aging, cardiovascular diseases and neural disorders. This study investigated the impact of naringenin on the glycation rate of glomerular basement membrane protein in diabetic rats to prevent nephropathy as a complication of diabetes. Fifty male Wistar rats were sorted into 2 main groups, Gr I (normal; n=10) and Gr II (Diabetic, n=40). Diabetes was induced in rats by injection of a single dose of 60 mg/kg, i.p. streptozocin (STZ). Diabetic rats were allocated to Gr IIa: (not treated), Gr IIb: treated with naringenin (50 mg/kg body weight - bw), Gr IIc: Diabetic treated with naringenin (100 mg/kg bw), Gr IId: Diabetic treated with metformin (100 mg/kg bw). Serum was utilized for the determination of malondialdehyde (MDA), glycated hemoglobin (HA1c), fructosamine, and total antioxidant activity. Kidney tissue was utilized for the determination of oxidative stress and advanced glycated end products (AGEs). Data obtained showed that naringenin supplementation in diabetic rats reduced the levels of serum MDA (p=0.0111), HA1c (p=0.0112), fructosamine (p=0.011), and improved antioxidant capacity (p=0.032). In addition, it reduced the level of AGEs (p=0.0008), and enhanced antioxidant enzymes (p<0.01134) compared with untreated. Diabetic rats showed a significant elevation in inflammatory markers tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), AGEs, decreased reduced glutathione, Superoxide Dismutase (SOD), Catalase (CAT) activity in kidney tissue versus control. The effect of naringenin was dose dependent, and improved these alterations versus untreated (p=0.0111). It was concluded that the inhibition of the formation of AGEs by naringenin in diabetics contributed to the protection against complications. Therefore, naringenin and its mechanisms of action is promising for development of safety and effective new antidiabetic agent. https://www.pagepressjournals.org/jbr/article/view/13600 |
| spellingShingle | Taha A. Kumosani Abeer Alnefayee Elie Barbour Mohammed Qari Tarek Ahmed Said S. Moselhy Impact of naringenin in inhibition of protein glycation in diabetic rats as a protection mechanism against nephropathy Journal of Biological Research |
| title | Impact of naringenin in inhibition of protein glycation in diabetic rats as a protection mechanism against nephropathy |
| title_full | Impact of naringenin in inhibition of protein glycation in diabetic rats as a protection mechanism against nephropathy |
| title_fullStr | Impact of naringenin in inhibition of protein glycation in diabetic rats as a protection mechanism against nephropathy |
| title_full_unstemmed | Impact of naringenin in inhibition of protein glycation in diabetic rats as a protection mechanism against nephropathy |
| title_short | Impact of naringenin in inhibition of protein glycation in diabetic rats as a protection mechanism against nephropathy |
| title_sort | impact of naringenin in inhibition of protein glycation in diabetic rats as a protection mechanism against nephropathy |
| url | https://www.pagepressjournals.org/jbr/article/view/13600 |
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