Using transcriptomics to investigate the effects of infant formulae on the growth and development of small intestinal organoids and Caco2 cells
Infant formula serves as a crucial breast milk alternative, yet its molecular mechanisms remain poorly understood. Small intestinal organoids (SIOs) are in vitro-cultured, multicellular structures and have been applied in diverse research fields. Nevertheless, SIOs have not been used in formula stud...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625002932 |
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| author | Xianli Wang Yuxin Jing Chengdong Zheng Chenxuan Huang Haiyang Yao Zening Wang Kainan Huang Yuanyuan Yan Shilong Jiang Jianguo Sun Qinggang Xie Yongjiu Zhang Xiaoguang Li Hui Wang |
| author_facet | Xianli Wang Yuxin Jing Chengdong Zheng Chenxuan Huang Haiyang Yao Zening Wang Kainan Huang Yuanyuan Yan Shilong Jiang Jianguo Sun Qinggang Xie Yongjiu Zhang Xiaoguang Li Hui Wang |
| author_sort | Xianli Wang |
| collection | DOAJ |
| description | Infant formula serves as a crucial breast milk alternative, yet its molecular mechanisms remain poorly understood. Small intestinal organoids (SIOs) are in vitro-cultured, multicellular structures and have been applied in diverse research fields. Nevertheless, SIOs have not been used in formula studies, nor have comparisons been made between SIOs and traditional models. This study pioneered using SIOs alongside Caco-2 cells to investigate formula's effects through transcriptomics and RT-qPCR. Formula upregulated proliferation, differentiation, and structural pathways in both models. Comparative analysis (GO/KEGG/IPA) revealed SIOs enhanced protein metabolism, mucin/peptide secretion, and immune responses versus Caco-2 cells, with key pathway genes identified. Notably, lactoferrin and Bifidobacterium lactis synergistically improved extracellular matrix development and immune signaling. These findings provide mechanistic evidence of formula's cellular impacts using SIOs, establishing their superiority over traditional models for nutritional research. Our work offers novel insights for formula optimization while demonstrating SIOs' potential as an advanced intestinal model system. |
| format | Article |
| id | doaj-art-fcc96578b04a4c2cad0f0bfa22dfd1e5 |
| institution | Matheson Library |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-fcc96578b04a4c2cad0f0bfa22dfd1e52025-07-22T04:12:42ZengElsevierJournal of Functional Foods1756-46462025-08-01131106951Using transcriptomics to investigate the effects of infant formulae on the growth and development of small intestinal organoids and Caco2 cellsXianli Wang0Yuxin Jing1Chengdong Zheng2Chenxuan Huang3Haiyang Yao4Zening Wang5Kainan Huang6Yuanyuan Yan7Shilong Jiang8Jianguo Sun9Qinggang Xie10Yongjiu Zhang11Xiaoguang Li12Hui Wang13School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaShanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaHeilongjiang Feihe Dairy Co., Ltd., C-16, 10A Jiuxianqiao Rd., Chaoyang, Beijing 100015, ChinaShanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaShanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaInstitutes of Biomedical Sciences, Fudan University, 131 Dongan Road, Shanghai 200032, ChinaCornell University, College of Agriculture and Life Science, Ithaca 14853, NY, United StatesSchool of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaHeilongjiang Feihe Dairy Co., Ltd., C-16, 10A Jiuxianqiao Rd., Chaoyang, Beijing 100015, ChinaHeilongjiang Feihe Dairy Co., Ltd., C-16, 10A Jiuxianqiao Rd., Chaoyang, Beijing 100015, ChinaHeilongjiang Feihe Dairy Co., Ltd., C-16, 10A Jiuxianqiao Rd., Chaoyang, Beijing 100015, ChinaHeilongjiang Feihe Dairy Co., Ltd., C-16, 10A Jiuxianqiao Rd., Chaoyang, Beijing 100015, China; Corresponding authors.State Key Laboratory of Systems Medicine for Cancer, Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Corresponding authors.State Key Laboratory of Systems Medicine for Cancer, Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Corresponding authors.Infant formula serves as a crucial breast milk alternative, yet its molecular mechanisms remain poorly understood. Small intestinal organoids (SIOs) are in vitro-cultured, multicellular structures and have been applied in diverse research fields. Nevertheless, SIOs have not been used in formula studies, nor have comparisons been made between SIOs and traditional models. This study pioneered using SIOs alongside Caco-2 cells to investigate formula's effects through transcriptomics and RT-qPCR. Formula upregulated proliferation, differentiation, and structural pathways in both models. Comparative analysis (GO/KEGG/IPA) revealed SIOs enhanced protein metabolism, mucin/peptide secretion, and immune responses versus Caco-2 cells, with key pathway genes identified. Notably, lactoferrin and Bifidobacterium lactis synergistically improved extracellular matrix development and immune signaling. These findings provide mechanistic evidence of formula's cellular impacts using SIOs, establishing their superiority over traditional models for nutritional research. Our work offers novel insights for formula optimization while demonstrating SIOs' potential as an advanced intestinal model system.http://www.sciencedirect.com/science/article/pii/S1756464625002932Infant formulaSmall intestinal organoidTranscriptomicsNutrition |
| spellingShingle | Xianli Wang Yuxin Jing Chengdong Zheng Chenxuan Huang Haiyang Yao Zening Wang Kainan Huang Yuanyuan Yan Shilong Jiang Jianguo Sun Qinggang Xie Yongjiu Zhang Xiaoguang Li Hui Wang Using transcriptomics to investigate the effects of infant formulae on the growth and development of small intestinal organoids and Caco2 cells Journal of Functional Foods Infant formula Small intestinal organoid Transcriptomics Nutrition |
| title | Using transcriptomics to investigate the effects of infant formulae on the growth and development of small intestinal organoids and Caco2 cells |
| title_full | Using transcriptomics to investigate the effects of infant formulae on the growth and development of small intestinal organoids and Caco2 cells |
| title_fullStr | Using transcriptomics to investigate the effects of infant formulae on the growth and development of small intestinal organoids and Caco2 cells |
| title_full_unstemmed | Using transcriptomics to investigate the effects of infant formulae on the growth and development of small intestinal organoids and Caco2 cells |
| title_short | Using transcriptomics to investigate the effects of infant formulae on the growth and development of small intestinal organoids and Caco2 cells |
| title_sort | using transcriptomics to investigate the effects of infant formulae on the growth and development of small intestinal organoids and caco2 cells |
| topic | Infant formula Small intestinal organoid Transcriptomics Nutrition |
| url | http://www.sciencedirect.com/science/article/pii/S1756464625002932 |
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