Identification and Pathogenicity Analysis of <i>Huaxiibacter chinensis</i> Qf-1 in Mink (<i>Neogale vison</i>)

Identification and Pathogenicity Analysis of <i>Huaxiibacter chinensis</i> Qf-1 in Mink (<i>Neogale vison</i>)

Mink (<i>Neogale vison</i>) is a commercially farmed animal of global importance. However, disease outbreaks during farming not only cause significant economic losses but also substantially increase the risk of zoonotic infections. The identification and characterization of pathogenic ba...

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Bibliographic Details
Main Authors: Yao Chen, Haotian Cai, Xiaoyang Wu, Xibao Wang, Yongquan Shang, Qinguo Wei, Weilai Sha, Yan Qi, Shuli Liu, Honghai Zhang
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Microorganisms
Subjects:
<i>Neogale vison</i>
<i>Huaxiibacter chinensis</i> Qf-1
culturomics
genome
pathogenicity
Online Access:https://www.mdpi.com/2076-2607/13/7/1604
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Summary:Mink (<i>Neogale vison</i>) is a commercially farmed animal of global importance. However, disease outbreaks during farming not only cause significant economic losses but also substantially increase the risk of zoonotic infections. The identification and characterization of pathogenic bacteria remain a major bottleneck restricting the development of healthy and sustainable mink farming. In this study, an LB medium was used to isolate a pale-white, rod-shaped, Gram-negative bacterial strain, Qf-1, from minks with pneumonia. Based on morphological characteristics, biochemical properties, 16S rRNA gene sequencing, and average nucleotide identity (ANI) analysis, strain Qf-1 was identified as <i>Huaxiibacter chinensis</i> Qf-1. Under laboratory conditions, <i>H. chinensis</i> Qf-1 induced typical pneumonia symptoms in Kunming mice. Furthermore, whole-genome sequencing of <i>H. chinensis</i> Qf-1 revealed its genome to be 4.77 Mb and to contain a single chromosome and one plasmid. The main virulence genes of <i>H. chinensis</i> Qf-1 were primarily associated with <i>flgB, flgC, flgG, aceA, hemL, tssC1, csgD, hofB, ppdD, hcpA</i>, and <i>vgrGA</i>, functioning in motility, biofilm formation, colonization ability, and secretion systems. Our findings contribute to a better understanding of their pathogenic mechanisms, thereby laying a theoretical foundation for further investigation into the complex interactions between gut microbiota and the host.
ISSN:2076-2607

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