Laboratory validation of targeted next-generation sequencing assay for pathogen detection in lower respiratory infection

Laboratory validation of targeted next-generation sequencing assay for pathogen detection in lower respiratory infection

ABSTRACT Lower respiratory tract infection (LRTI) is a serious global public health issue and poses significant challenges for detection and diagnosis. We validated the detection performance of targeted next-generation sequencing (tNGS) based on multiplex PCR using simulated microbial sample panels...

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Bibliographic Details
Main Authors: Jing Yang, Yan Wang, Li Yang, Jun Wu
Format: Article
Language:English
Published: American Society for Microbiology 2025-07-01
Series:Microbiology Spectrum
Subjects:
tNGS
lower respiratory infection
laboratory validation
diagnosis
infection
Online Access:https://journals.asm.org/doi/10.1128/spectrum.01751-24
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