Streptomyces coelicolor-plant association facilitates ergothioneine uptake in Triticum aestivum
The growing market of agricultural biologicals as alternatives to synthetic crop chemicals is driven by their ability to improve soil health, reduce carbon footprints, enhance crop yield and quality, and help counter declining protein levels in cereal crops linked to climate change and soil degradat...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1637050/full |
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| author | Alexandra Pipinos Alexandra Pipinos Jinjun Kan Andrew Smith Gladis Zinati Harsh Bais Harsh Bais |
| author_facet | Alexandra Pipinos Alexandra Pipinos Jinjun Kan Andrew Smith Gladis Zinati Harsh Bais Harsh Bais |
| author_sort | Alexandra Pipinos |
| collection | DOAJ |
| description | The growing market of agricultural biologicals as alternatives to synthetic crop chemicals is driven by their ability to improve soil health, reduce carbon footprints, enhance crop yield and quality, and help counter declining protein levels in cereal crops linked to climate change and soil degradation. Ergothioneine (EGT), an amino acid with recognized nutraceutical and micronutrient properties, has gained popularity for its anti-inflammatory and antimicrobial properties on human health. While plants and humans cannot biosynthesize EGT, its production by Streptomyces coelicolor presents as a promising bio-stimulant to support overall plant and human health. Our study investigates the potential for S. coelicolor M145 to enhance EGT levels in spring wheat (Triticum aestivum). Results confirmed successful EGT extraction from bacterial cell extracts and plant tissues. The bacterial cells grown in nutrient rich media showed significant levels of EGT post day 7 of incubation, with an average of 0.32 μM of EGT, while bacteria growing in the limiting nutrient condition produced an average of 0.27 μM EGT. In parallel, wheat plants inoculated with S. coelicolor and extracted for EGT on day 10 post incubation, showed higher shoot EGT content (0.1168 ± 0.071 μM) in bacteria treated plants. Additionally, a fluorescent confocal microscopy staining and imaging protocol showed bacterial colonization on T. aestivum and its potential as a root endophyte. Following root inoculation, S. coelicolor was observed to inhabit roots, shoots, and internodes of T. aestivum, suggesting its potential endophytic lifestyle on host plants. Our data showed that S. coelicolor-associated wheat plants produce EGT in planta. Overall, our findings establish a direct link between soil and human health through rhizosphere colonization by S. coelicolor and in planta production of EGT, suggesting an alternate route to enhance protein concentration in crop plants. |
| format | Article |
| id | doaj-art-f9c7e8c7adb54d3398b7e8776c989330 |
| institution | Matheson Library |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-f9c7e8c7adb54d3398b7e8776c9893302025-07-18T05:30:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.16370501637050Streptomyces coelicolor-plant association facilitates ergothioneine uptake in Triticum aestivumAlexandra Pipinos0Alexandra Pipinos1Jinjun Kan2Andrew Smith3Gladis Zinati4Harsh Bais5Harsh Bais6Department of Plant and Soil Sciences, University of Delaware, Newark, DE, United StatesAP Biopharma, University of Delaware, Newark, DE, United StatesStroud Water Research Center, Avondale, PA, United StatesRodale Institute, Kutztown, PA, United StatesRodale Institute, Kutztown, PA, United StatesDepartment of Plant and Soil Sciences, University of Delaware, Newark, DE, United StatesAP Biopharma, University of Delaware, Newark, DE, United StatesThe growing market of agricultural biologicals as alternatives to synthetic crop chemicals is driven by their ability to improve soil health, reduce carbon footprints, enhance crop yield and quality, and help counter declining protein levels in cereal crops linked to climate change and soil degradation. Ergothioneine (EGT), an amino acid with recognized nutraceutical and micronutrient properties, has gained popularity for its anti-inflammatory and antimicrobial properties on human health. While plants and humans cannot biosynthesize EGT, its production by Streptomyces coelicolor presents as a promising bio-stimulant to support overall plant and human health. Our study investigates the potential for S. coelicolor M145 to enhance EGT levels in spring wheat (Triticum aestivum). Results confirmed successful EGT extraction from bacterial cell extracts and plant tissues. The bacterial cells grown in nutrient rich media showed significant levels of EGT post day 7 of incubation, with an average of 0.32 μM of EGT, while bacteria growing in the limiting nutrient condition produced an average of 0.27 μM EGT. In parallel, wheat plants inoculated with S. coelicolor and extracted for EGT on day 10 post incubation, showed higher shoot EGT content (0.1168 ± 0.071 μM) in bacteria treated plants. Additionally, a fluorescent confocal microscopy staining and imaging protocol showed bacterial colonization on T. aestivum and its potential as a root endophyte. Following root inoculation, S. coelicolor was observed to inhabit roots, shoots, and internodes of T. aestivum, suggesting its potential endophytic lifestyle on host plants. Our data showed that S. coelicolor-associated wheat plants produce EGT in planta. Overall, our findings establish a direct link between soil and human health through rhizosphere colonization by S. coelicolor and in planta production of EGT, suggesting an alternate route to enhance protein concentration in crop plants.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1637050/fullendophyteergothioneinerhizosphererootswheat |
| spellingShingle | Alexandra Pipinos Alexandra Pipinos Jinjun Kan Andrew Smith Gladis Zinati Harsh Bais Harsh Bais Streptomyces coelicolor-plant association facilitates ergothioneine uptake in Triticum aestivum Frontiers in Microbiology endophyte ergothioneine rhizosphere roots wheat |
| title | Streptomyces coelicolor-plant association facilitates ergothioneine uptake in Triticum aestivum |
| title_full | Streptomyces coelicolor-plant association facilitates ergothioneine uptake in Triticum aestivum |
| title_fullStr | Streptomyces coelicolor-plant association facilitates ergothioneine uptake in Triticum aestivum |
| title_full_unstemmed | Streptomyces coelicolor-plant association facilitates ergothioneine uptake in Triticum aestivum |
| title_short | Streptomyces coelicolor-plant association facilitates ergothioneine uptake in Triticum aestivum |
| title_sort | streptomyces coelicolor plant association facilitates ergothioneine uptake in triticum aestivum |
| topic | endophyte ergothioneine rhizosphere roots wheat |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1637050/full |
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