A Comprehensive Investigation of Potential Bacterial Pathogens in Largemouth Bass (<i>Micropterus salmoides</i>)
In the study, a comprehensive investigation on potential bacterial pathogens affecting largemouth bass (<i>Micropterus salmoides</i>) was performed. Monthly surveys were conducted from April to October 2024. Diseased largemouth bass exhibited diverse clinical symptoms, such as rot of gil...
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2025-06-01
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| author | Yun-Yao Tu Qun Lu Na Zhang Jie Leng Qin Yang Jie Yu Cheng-Ke Zhu Tao He Jian-Yong Hu Ming-Ji Lv Song Zhu |
| author_facet | Yun-Yao Tu Qun Lu Na Zhang Jie Leng Qin Yang Jie Yu Cheng-Ke Zhu Tao He Jian-Yong Hu Ming-Ji Lv Song Zhu |
| author_sort | Yun-Yao Tu |
| collection | DOAJ |
| description | In the study, a comprehensive investigation on potential bacterial pathogens affecting largemouth bass (<i>Micropterus salmoides</i>) was performed. Monthly surveys were conducted from April to October 2024. Diseased largemouth bass exhibited diverse clinical symptoms, such as rot of gill and fin, ulcers on body surface, and petechial hemorrhages in liver. Following isolation and identification, a total of 21 potential bacterial pathogens (numbered strain 1 to 21, respectively) were identified. The genus <i>Aeromonas</i> had the highest proportion (67.14%), among which the frequency of <i>Aeromonas veronii</i> was 24.60%. TEM analysis revealed that the bacterial strains exhibited three predominant shapes (rod-shaped, spherical, and curved) with length ranging from 0.5 to 3 μm. Flagellar structures were observed in strains 1–4, 6–8, 11–17, and 19–21, with variations in number and growth sites. Three isolates (strains 9, 10, 18) demonstrated Gram-positive characteristic, and strains 5, 11, and 18 have capsule structures. Strains 5, 9, 10, and 18 were non-motile, and strains 1–4, 6, 7, 9–11, 16–18, and 21 exhibited β-hemolysis. Physiological and biochemical characteristics of the 21 bacterial isolates were comprehensively analyzed. Antibiotic sensitivity testing revealed that florfenicol and enrofloxacin exhibited excellent antibacterial effects. These data will enrich the potential bacterial diseases information and promote the healthy development of the largemouth bass industry. |
| format | Article |
| id | doaj-art-f9b926b5ff0147dab5e43d4c0fcde944 |
| institution | Matheson Library |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-f9b926b5ff0147dab5e43d4c0fcde9442025-06-25T14:12:50ZengMDPI AGMicroorganisms2076-26072025-06-01136141310.3390/microorganisms13061413A Comprehensive Investigation of Potential Bacterial Pathogens in Largemouth Bass (<i>Micropterus salmoides</i>)Yun-Yao Tu0Qun Lu1Na Zhang2Jie Leng3Qin Yang4Jie Yu5Cheng-Ke Zhu6Tao He7Jian-Yong Hu8Ming-Ji Lv9Song Zhu10College of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaXinjiang Fisheries Research Institute, Scientific Observing and Experimental Station of Fishery Resources and Environment in Northwest China, Ministry of Agriculture, Urumqi 830000, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaCollege of Fisheries, Southwest University, Chongqing 400715, ChinaIn the study, a comprehensive investigation on potential bacterial pathogens affecting largemouth bass (<i>Micropterus salmoides</i>) was performed. Monthly surveys were conducted from April to October 2024. Diseased largemouth bass exhibited diverse clinical symptoms, such as rot of gill and fin, ulcers on body surface, and petechial hemorrhages in liver. Following isolation and identification, a total of 21 potential bacterial pathogens (numbered strain 1 to 21, respectively) were identified. The genus <i>Aeromonas</i> had the highest proportion (67.14%), among which the frequency of <i>Aeromonas veronii</i> was 24.60%. TEM analysis revealed that the bacterial strains exhibited three predominant shapes (rod-shaped, spherical, and curved) with length ranging from 0.5 to 3 μm. Flagellar structures were observed in strains 1–4, 6–8, 11–17, and 19–21, with variations in number and growth sites. Three isolates (strains 9, 10, 18) demonstrated Gram-positive characteristic, and strains 5, 11, and 18 have capsule structures. Strains 5, 9, 10, and 18 were non-motile, and strains 1–4, 6, 7, 9–11, 16–18, and 21 exhibited β-hemolysis. Physiological and biochemical characteristics of the 21 bacterial isolates were comprehensively analyzed. Antibiotic sensitivity testing revealed that florfenicol and enrofloxacin exhibited excellent antibacterial effects. These data will enrich the potential bacterial diseases information and promote the healthy development of the largemouth bass industry.https://www.mdpi.com/2076-2607/13/6/1413largemouth bassbacterial pathogensantibiotic sensitivityhealthful aquaculture |
| spellingShingle | Yun-Yao Tu Qun Lu Na Zhang Jie Leng Qin Yang Jie Yu Cheng-Ke Zhu Tao He Jian-Yong Hu Ming-Ji Lv Song Zhu A Comprehensive Investigation of Potential Bacterial Pathogens in Largemouth Bass (<i>Micropterus salmoides</i>) Microorganisms largemouth bass bacterial pathogens antibiotic sensitivity healthful aquaculture |
| title | A Comprehensive Investigation of Potential Bacterial Pathogens in Largemouth Bass (<i>Micropterus salmoides</i>) |
| title_full | A Comprehensive Investigation of Potential Bacterial Pathogens in Largemouth Bass (<i>Micropterus salmoides</i>) |
| title_fullStr | A Comprehensive Investigation of Potential Bacterial Pathogens in Largemouth Bass (<i>Micropterus salmoides</i>) |
| title_full_unstemmed | A Comprehensive Investigation of Potential Bacterial Pathogens in Largemouth Bass (<i>Micropterus salmoides</i>) |
| title_short | A Comprehensive Investigation of Potential Bacterial Pathogens in Largemouth Bass (<i>Micropterus salmoides</i>) |
| title_sort | comprehensive investigation of potential bacterial pathogens in largemouth bass i micropterus salmoides i |
| topic | largemouth bass bacterial pathogens antibiotic sensitivity healthful aquaculture |
| url | https://www.mdpi.com/2076-2607/13/6/1413 |
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