Cloning and sequencing of gene encoding hyaluronidase from the venom of Apis mellifera
The venomous hyaluronidase (AmHya) gene of Italian honey bee (Apis mellifera) collected in China was amplified by RT-PCR from total RNA of venom glands of the worker bee. Sequence analysis showed the gene was composed of 1 149 nucleotides. The alignment of the amino acid sequence of AmHya gene and 6...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2002-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2002.03.0289 |
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| Summary: | The venomous hyaluronidase (AmHya) gene of Italian honey bee (Apis mellifera) collected in China was amplified by RT-PCR from total RNA of venom glands of the worker bee. Sequence analysis showed the gene was composed of 1 149 nucleotides. The alignment of the amino acid sequence of AmHya gene and 6 Hya genes from other organisms showed that AmHya is most closely related to the AcHya of Chinese honeybee (A. cerana cerana) with 91% amino acid level identity. A phylogenetic tree of 7 Hyaluronidases was drawn by using GENETYX program, and molecular structures and functions of these ensymes were compared and analysed. |
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| ISSN: | 1008-9209 2097-5155 |