Study on some problems in breeding insect-resistant Bt transgenic Japonica rice
Using the Bt rice “KMD” as the insect-resistant donor, three transgenic Japonica rice lines with high yield and insect resistance were successfully developed from crosses between the Bt rice and Japonica rice variety after pedigree selections lasting eight generations through GUS tissue staining-aid...
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Format: | Article |
Language: | English |
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Zhejiang University Press
2003-09-01
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Series: | 浙江大学学报. 农业与生命科学版 |
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Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.05.0499 |
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author | SHEN Sheng-quan WU Dian-xing CUI Hai-rui XIA Ying-wu SHU Qing-yao |
author_facet | SHEN Sheng-quan WU Dian-xing CUI Hai-rui XIA Ying-wu SHU Qing-yao |
author_sort | SHEN Sheng-quan |
collection | DOAJ |
description | Using the Bt rice “KMD” as the insect-resistant donor, three transgenic Japonica rice lines with high yield and insect resistance were successfully developed from crosses between the Bt rice and Japonica rice variety after pedigree selections lasting eight generations through GUS tissue staining-aided selection as well as laboratory and field insect-resistant evaluation. This fact indicates that the poor agronomic traits in the original transgenic rice plants can be improved through the conventional crossing breeding techniques. The effects of four kinds of breeding techniques, which involved screening the insect-resistant transgenic rice plants as revealed by GUS tissue staining, agronomic traits selection, bulk selection, field insect-resistance selection, were comparatively studied. The result showed that it is desirable to create insect-resistant Bt transgenic new Japonica rice via integral utilization of pedigree method and GUS tissue staining-aided selection. |
format | Article |
id | doaj-art-f8b3fbdf7c50421c94c27136ab8ecd8d |
institution | Matheson Library |
issn | 1008-9209 2097-5155 |
language | English |
publishDate | 2003-09-01 |
publisher | Zhejiang University Press |
record_format | Article |
series | 浙江大学学报. 农业与生命科学版 |
spelling | doaj-art-f8b3fbdf7c50421c94c27136ab8ecd8d2025-08-01T05:38:49ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552003-09-012949950310.3785/1008-9209.2003.05.049910089209Study on some problems in breeding insect-resistant Bt transgenic Japonica riceSHEN Sheng-quanWU Dian-xingCUI Hai-ruiXIA Ying-wuSHU Qing-yaoUsing the Bt rice “KMD” as the insect-resistant donor, three transgenic Japonica rice lines with high yield and insect resistance were successfully developed from crosses between the Bt rice and Japonica rice variety after pedigree selections lasting eight generations through GUS tissue staining-aided selection as well as laboratory and field insect-resistant evaluation. This fact indicates that the poor agronomic traits in the original transgenic rice plants can be improved through the conventional crossing breeding techniques. The effects of four kinds of breeding techniques, which involved screening the insect-resistant transgenic rice plants as revealed by GUS tissue staining, agronomic traits selection, bulk selection, field insect-resistance selection, were comparatively studied. The result showed that it is desirable to create insect-resistant Bt transgenic new Japonica rice via integral utilization of pedigree method and GUS tissue staining-aided selection.https://www.academax.com/doi/10.3785/1008-9209.2003.05.0499riceBt genetransgenecrossing breedinginsect resistance |
spellingShingle | SHEN Sheng-quan WU Dian-xing CUI Hai-rui XIA Ying-wu SHU Qing-yao Study on some problems in breeding insect-resistant Bt transgenic Japonica rice 浙江大学学报. 农业与生命科学版 rice Bt gene transgene crossing breeding insect resistance |
title | Study on some problems in breeding insect-resistant Bt transgenic Japonica rice |
title_full | Study on some problems in breeding insect-resistant Bt transgenic Japonica rice |
title_fullStr | Study on some problems in breeding insect-resistant Bt transgenic Japonica rice |
title_full_unstemmed | Study on some problems in breeding insect-resistant Bt transgenic Japonica rice |
title_short | Study on some problems in breeding insect-resistant Bt transgenic Japonica rice |
title_sort | study on some problems in breeding insect resistant bt transgenic japonica rice |
topic | rice Bt gene transgene crossing breeding insect resistance |
url | https://www.academax.com/doi/10.3785/1008-9209.2003.05.0499 |
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