The Nutritional Gene Expression Regulation Potential of a Lysolecithin-Based Product
Lysolecithin is a performance-enhancing product for livestock. Lysolecithins contain functional phospholipids (PLs) and lysophospholipids (LPLs) and have been used in monogastric feed formulations because they can enhance lipid emulsification, digestion, and absorption (surface chemistry). Another u...
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MDPI AG
2025-07-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/47/7/548 |
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| author | Veerle Van Hoeck Riet Spaepen Bart Forier |
| author_facet | Veerle Van Hoeck Riet Spaepen Bart Forier |
| author_sort | Veerle Van Hoeck |
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| description | Lysolecithin is a performance-enhancing product for livestock. Lysolecithins contain functional phospholipids (PLs) and lysophospholipids (LPLs) and have been used in monogastric feed formulations because they can enhance lipid emulsification, digestion, and absorption (surface chemistry). Another underexplored aspect is that lysolecithin mixtures can serve as signaling via so-called nutritional gene expression-regulating action. The scope of this study was to fully understand the potential of a lysolecithin source derived from soybeans to influence intestinal nutrient transport in the intestinal tract. In this context, in vitro cell culture data with intestinal Caco-2 cells revealed that a lysolecithin-based product can significantly improve intestinal cell viability. Furthermore, a Transwell culture experiment showed that lysolecithins can significantly trigger gene expression. The most significantly affected genes could be correlated with G-coupled protein cascades. Enrichment analyses showed that amino acid transport and lipid metabolism pathways are significantly affected. Furthermore, the polarized cell culture revealed that the studied lysolecithin could affect the abundance of metabolites/nutrients in the basolateral compartment when applied apically, indicating that its action exceeds surface chemistry. In conclusion, the data on intestinal cell viability, gene expression, and metabolite abundance seem to reveal the bioactivities of lysolecithin. The latter data suggest that the specific lysolecithin source used here is more than a biosurfactant; more specifically, it seems to be a potent bioactive mixture of amphiphilic compounds triggering cell signaling pathways. |
| format | Article |
| id | doaj-art-f8aae5dba9304b55acf0a5121e5a82f5 |
| institution | Matheson Library |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-f8aae5dba9304b55acf0a5121e5a82f52025-07-25T13:18:46ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-07-0147754810.3390/cimb47070548The Nutritional Gene Expression Regulation Potential of a Lysolecithin-Based ProductVeerle Van Hoeck0Riet Spaepen1Bart Forier2Kemin Europa N.V., 2200 Herentals, BelgiumKemin Europa N.V., 2200 Herentals, BelgiumKemin Europa N.V., 2200 Herentals, BelgiumLysolecithin is a performance-enhancing product for livestock. Lysolecithins contain functional phospholipids (PLs) and lysophospholipids (LPLs) and have been used in monogastric feed formulations because they can enhance lipid emulsification, digestion, and absorption (surface chemistry). Another underexplored aspect is that lysolecithin mixtures can serve as signaling via so-called nutritional gene expression-regulating action. The scope of this study was to fully understand the potential of a lysolecithin source derived from soybeans to influence intestinal nutrient transport in the intestinal tract. In this context, in vitro cell culture data with intestinal Caco-2 cells revealed that a lysolecithin-based product can significantly improve intestinal cell viability. Furthermore, a Transwell culture experiment showed that lysolecithins can significantly trigger gene expression. The most significantly affected genes could be correlated with G-coupled protein cascades. Enrichment analyses showed that amino acid transport and lipid metabolism pathways are significantly affected. Furthermore, the polarized cell culture revealed that the studied lysolecithin could affect the abundance of metabolites/nutrients in the basolateral compartment when applied apically, indicating that its action exceeds surface chemistry. In conclusion, the data on intestinal cell viability, gene expression, and metabolite abundance seem to reveal the bioactivities of lysolecithin. The latter data suggest that the specific lysolecithin source used here is more than a biosurfactant; more specifically, it seems to be a potent bioactive mixture of amphiphilic compounds triggering cell signaling pathways.https://www.mdpi.com/1467-3045/47/7/548lysolecithinCaco-2 cellsnutritional regulation of gene expressionphospholipidslysophospholipids |
| spellingShingle | Veerle Van Hoeck Riet Spaepen Bart Forier The Nutritional Gene Expression Regulation Potential of a Lysolecithin-Based Product Current Issues in Molecular Biology lysolecithin Caco-2 cells nutritional regulation of gene expression phospholipids lysophospholipids |
| title | The Nutritional Gene Expression Regulation Potential of a Lysolecithin-Based Product |
| title_full | The Nutritional Gene Expression Regulation Potential of a Lysolecithin-Based Product |
| title_fullStr | The Nutritional Gene Expression Regulation Potential of a Lysolecithin-Based Product |
| title_full_unstemmed | The Nutritional Gene Expression Regulation Potential of a Lysolecithin-Based Product |
| title_short | The Nutritional Gene Expression Regulation Potential of a Lysolecithin-Based Product |
| title_sort | nutritional gene expression regulation potential of a lysolecithin based product |
| topic | lysolecithin Caco-2 cells nutritional regulation of gene expression phospholipids lysophospholipids |
| url | https://www.mdpi.com/1467-3045/47/7/548 |
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