Novel Probiotic Strain <i>Lactiplantibacillus plantarum</i> CNTA 628 Modulates Lipid Metabolism and Improves Healthspan in <i>C. elegans</i>
The call for new approaches to prevent and treat metabolic syndrome-related diseases has led to research on the use of lacto-fermentative probiotics with beneficial metabolic properties like <i>Lactobacilli</i>. Here, we characterize the probiotic properties of a novel strain, <i>L...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/15/14/8007 |
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| Summary: | The call for new approaches to prevent and treat metabolic syndrome-related diseases has led to research on the use of lacto-fermentative probiotics with beneficial metabolic properties like <i>Lactobacilli</i>. Here, we characterize the probiotic properties of a novel strain, <i>Lactiplantibacillus plantarum</i> CNTA 628, and investigate its potential anti-obesity and health-promoting activities in the <i>Caenorhabditis elegans</i> model, additionally elucidating the molecular mechanisms involved. <i>Lactiplantibacillus plantarum</i> CNTA 628 exhibited sensitivity to the entire spectrum of antibiotics analyzed, gastric and intestinal resistance in vitro, β-galactosidase and bile-salt hydrolysate activities, and the capacity to form biofilms and produce SCFAs. In addition, it reduced the binding of the pathogenic <i>E. coli</i> O157:H7 to intestinal epithelial cells (Caco-2) and exerted immune-modulating effects in cellular models. Supplementation with this probiotic significantly reduced <i>C. elegans</i> fat accumulation by more than 18% under control and high-glucose conditions, lowered senescence, improved oxidative stress, and significantly enhanced lifespan without affecting the development of the worms. Gene expression analyses evidenced that <i>L. plantarum</i> CNTA 628 plays a role in regulating <i>daf-22</i> and <i>maoc-1</i> gene expression, both linked to beta-oxidation pathways. Our results demonstrate the health-benefiting properties of this novel strain and suggest its potential as probiotic candidate for the prevention and treatment of metabolic syndrome-related conditions. |
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| ISSN: | 2076-3417 |