Molecular profile of Epstein–Barr virus in representatives of four ethnic groups of Russia: virus types, <i>LMP1</i> gene variability and malignancies
Rationale. The discovery of two Epstein–Barr virus (EBV) types, EBV-1 and EBV-2, which differ in their transforming abilities stimulated their study in various populations in order to elucidate the relationship with malignant neoplasms.Aim. To study EBV-1 and EBV-2 in representatives of four ethnic...
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ABV-press
2023-12-01
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| Series: | Успехи молекулярной онкологии |
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| Online Access: | https://umo.abvpress.ru/jour/article/view/613 |
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| author | K. V. Smirnova A. K. Lubenskaya N. B. Senyuta T. E. Dushenkina V. E. Gurtsevitch |
| author_facet | K. V. Smirnova A. K. Lubenskaya N. B. Senyuta T. E. Dushenkina V. E. Gurtsevitch |
| author_sort | K. V. Smirnova |
| collection | DOAJ |
| description | Rationale. The discovery of two Epstein–Barr virus (EBV) types, EBV-1 and EBV-2, which differ in their transforming abilities stimulated their study in various populations in order to elucidate the relationship with malignant neoplasms.Aim. To study EBV-1 and EBV-2 in representatives of four ethnic groups of Russia, sequence analysis of the LMP1 oncogene in virus isolates, and search for a correlation between the types of EBV and the incidence of certain types of malignant tumors in four ethnic groups.Materials and methods. From total DNA obtained from oral washings of Adygeans, Kalmyks, Tatars and Slavs, representatives of 3 Russian republics and the Moscow region, EBV DNA was amplified; its presence and concentration were analyzed by nested PCR, and each product was examined for the virus type and LMP1 variant. Types of EBV found in representatives of In four ethnic groups were compared with the incidence rates of malignant tumors, among which EBV-associated cases may occur, in population of three republics and the Moscow region.Results. It was observed that higher gastric cancer and lymphomas morbidity among Tatarstan and Moscow region populations (compared to populations of Adygea and Kalmykia) correlated with predominance in these ethnic groups – Tatars and Slavs – EBV-1 type having transforming potential in vitro. In Adygeis, non-transforming EBV-2 type was prevalent, and Kalmyks were infected with both types in approximately equal proportion. however, differences between tumor morbidities between the compared groups were statistically insignificant (p >0.05). The identified spectrum of LMP1 variants, as well as the results of their sequencing, show genetic relations between EBV strains circulating in the studied ethnic groups.Conclusion. Study of EBV isolates for the first time performed in 4 ethnic groups of this country showed differences in distribution of the types of this virus but there was no correlation with tumor morbidity. |
| format | Article |
| id | doaj-art-f7435085dd7f4565aebcb7e43e8950d5 |
| institution | Matheson Library |
| issn | 2313-805X 2413-3787 |
| language | Russian |
| publishDate | 2023-12-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Успехи молекулярной онкологии |
| spelling | doaj-art-f7435085dd7f4565aebcb7e43e8950d52025-08-04T14:04:37ZrusABV-pressУспехи молекулярной онкологии2313-805X2413-37872023-12-0110411612310.17650/2313-805X-2023-10-4-116-123310Molecular profile of Epstein–Barr virus in representatives of four ethnic groups of Russia: virus types, <i>LMP1</i> gene variability and malignanciesK. V. Smirnova0A. K. Lubenskaya1N. B. Senyuta2T. E. Dushenkina3V. E. Gurtsevitch4Research Institute of Carcinogenesis of the N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia; The Peoples’ Friendship University of RussiaResearch Institute of Carcinogenesis of the N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of RussiaResearch Institute of Carcinogenesis of the N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of RussiaResearch Institute of Carcinogenesis of the N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of RussiaResearch Institute of Carcinogenesis of the N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of RussiaRationale. The discovery of two Epstein–Barr virus (EBV) types, EBV-1 and EBV-2, which differ in their transforming abilities stimulated their study in various populations in order to elucidate the relationship with malignant neoplasms.Aim. To study EBV-1 and EBV-2 in representatives of four ethnic groups of Russia, sequence analysis of the LMP1 oncogene in virus isolates, and search for a correlation between the types of EBV and the incidence of certain types of malignant tumors in four ethnic groups.Materials and methods. From total DNA obtained from oral washings of Adygeans, Kalmyks, Tatars and Slavs, representatives of 3 Russian republics and the Moscow region, EBV DNA was amplified; its presence and concentration were analyzed by nested PCR, and each product was examined for the virus type and LMP1 variant. Types of EBV found in representatives of In four ethnic groups were compared with the incidence rates of malignant tumors, among which EBV-associated cases may occur, in population of three republics and the Moscow region.Results. It was observed that higher gastric cancer and lymphomas morbidity among Tatarstan and Moscow region populations (compared to populations of Adygea and Kalmykia) correlated with predominance in these ethnic groups – Tatars and Slavs – EBV-1 type having transforming potential in vitro. In Adygeis, non-transforming EBV-2 type was prevalent, and Kalmyks were infected with both types in approximately equal proportion. however, differences between tumor morbidities between the compared groups were statistically insignificant (p >0.05). The identified spectrum of LMP1 variants, as well as the results of their sequencing, show genetic relations between EBV strains circulating in the studied ethnic groups.Conclusion. Study of EBV isolates for the first time performed in 4 ethnic groups of this country showed differences in distribution of the types of this virus but there was no correlation with tumor morbidity.https://umo.abvpress.ru/jour/article/view/613epstein–barr virusepstein–barr virus typeslatent membrane protein 1sequence analysisadyghekalmykstatarsslavsreal-time polymerase chain reactionmalignant tumors |
| spellingShingle | K. V. Smirnova A. K. Lubenskaya N. B. Senyuta T. E. Dushenkina V. E. Gurtsevitch Molecular profile of Epstein–Barr virus in representatives of four ethnic groups of Russia: virus types, <i>LMP1</i> gene variability and malignancies Успехи молекулярной онкологии epstein–barr virus epstein–barr virus types latent membrane protein 1 sequence analysis adyghe kalmyks tatars slavs real-time polymerase chain reaction malignant tumors |
| title | Molecular profile of Epstein–Barr virus in representatives of four ethnic groups of Russia: virus types, <i>LMP1</i> gene variability and malignancies |
| title_full | Molecular profile of Epstein–Barr virus in representatives of four ethnic groups of Russia: virus types, <i>LMP1</i> gene variability and malignancies |
| title_fullStr | Molecular profile of Epstein–Barr virus in representatives of four ethnic groups of Russia: virus types, <i>LMP1</i> gene variability and malignancies |
| title_full_unstemmed | Molecular profile of Epstein–Barr virus in representatives of four ethnic groups of Russia: virus types, <i>LMP1</i> gene variability and malignancies |
| title_short | Molecular profile of Epstein–Barr virus in representatives of four ethnic groups of Russia: virus types, <i>LMP1</i> gene variability and malignancies |
| title_sort | molecular profile of epstein barr virus in representatives of four ethnic groups of russia virus types i lmp1 i gene variability and malignancies |
| topic | epstein–barr virus epstein–barr virus types latent membrane protein 1 sequence analysis adyghe kalmyks tatars slavs real-time polymerase chain reaction malignant tumors |
| url | https://umo.abvpress.ru/jour/article/view/613 |
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