PCR-DGGE Based Biodiveristy, Changeability and Genetic Similarity Analysis of Bacterial Community in Sequencing Batch Reactors Dealing with Reject Water
Reject water, produced during stabilization and dewatering of activated sludge, contains a high load of biogenic compounds which are returned to bioreactors, so there is a necessity for its treatment within biological systems. The efficacy of such performance depends mainly on the activated sludge c...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institute of Fundamental Technological Research
2014-11-01
|
| Series: | Engineering Transactions |
| Subjects: | |
| Online Access: | https://et.ippt.pan.pl/index.php/et/article/view/35 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839633503964102656 |
|---|---|
| author | Aleksandra ZIEMBIŃSKA-BUCZYŃSKA Grzegorz CEMA Marta SZPINDOR Anna MERESTA Lesław PŁONKA |
| author_facet | Aleksandra ZIEMBIŃSKA-BUCZYŃSKA Grzegorz CEMA Marta SZPINDOR Anna MERESTA Lesław PŁONKA |
| author_sort | Aleksandra ZIEMBIŃSKA-BUCZYŃSKA |
| collection | DOAJ |
| description | Reject water, produced during stabilization and dewatering of activated sludge, contains a high load of biogenic compounds which are returned to bioreactors, so there is a necessity for its treatment within biological systems. The efficacy of such performance depends mainly on the activated sludge composition responsible for the biochemical processes. For biocenosis diversity and changeability, PCR-DGGE is the most commonly used tool. In this article we monitor the activated sludge bacterial communities of two sequencing batch reactors (SBRs) dealing with reject water but varying in their operational parameters. A physico-chemical analysis of the SBRs performance was also done. We used two different PCR primers sets to present a total overview of the changeability in complex microbial biocenosis during wastewater treatment. For biodiversity monitoring 16S rRNA gene fragments amplified with 338f-GC/518r primers appeared to be more suitable than the fragments amplified with 968f-GC/1401r – the index was higher though the changes were proportional for both cases. The results were confirmed with genetic similarity analysis presented as dendrograms. |
| format | Article |
| id | doaj-art-f53c2c8a44e54763bbb0ddc3bbf5549b |
| institution | Matheson Library |
| issn | 0867-888X 2450-8071 |
| language | English |
| publishDate | 2014-11-01 |
| publisher | Institute of Fundamental Technological Research |
| record_format | Article |
| series | Engineering Transactions |
| spelling | doaj-art-f53c2c8a44e54763bbb0ddc3bbf5549b2025-07-11T04:54:46ZengInstitute of Fundamental Technological ResearchEngineering Transactions0867-888X2450-80712014-11-0162210.24423/engtrans.35.2014PCR-DGGE Based Biodiveristy, Changeability and Genetic Similarity Analysis of Bacterial Community in Sequencing Batch Reactors Dealing with Reject WaterAleksandra ZIEMBIŃSKA-BUCZYŃSKA0Grzegorz CEMA1Marta SZPINDOR2Anna MERESTA3Lesław PŁONKA4The Silesian University of Technology, Environmental Biotechnology Department, GliwiceThe Silesian University of Technology, Environmental Biotechnology Department, GliwiceThe Silesian University of Technology, Environmental Biotechnology Department, GliwiceThe Silesian University of Technology, Environmental Biotechnology Department, GliwiceThe Silesian University of Technology, Environmental Biotechnology Department, GliwiceReject water, produced during stabilization and dewatering of activated sludge, contains a high load of biogenic compounds which are returned to bioreactors, so there is a necessity for its treatment within biological systems. The efficacy of such performance depends mainly on the activated sludge composition responsible for the biochemical processes. For biocenosis diversity and changeability, PCR-DGGE is the most commonly used tool. In this article we monitor the activated sludge bacterial communities of two sequencing batch reactors (SBRs) dealing with reject water but varying in their operational parameters. A physico-chemical analysis of the SBRs performance was also done. We used two different PCR primers sets to present a total overview of the changeability in complex microbial biocenosis during wastewater treatment. For biodiversity monitoring 16S rRNA gene fragments amplified with 338f-GC/518r primers appeared to be more suitable than the fragments amplified with 968f-GC/1401r – the index was higher though the changes were proportional for both cases. The results were confirmed with genetic similarity analysis presented as dendrograms.https://et.ippt.pan.pl/index.php/et/article/view/35PCR-DGGESBRbacterial community monitoringreject water |
| spellingShingle | Aleksandra ZIEMBIŃSKA-BUCZYŃSKA Grzegorz CEMA Marta SZPINDOR Anna MERESTA Lesław PŁONKA PCR-DGGE Based Biodiveristy, Changeability and Genetic Similarity Analysis of Bacterial Community in Sequencing Batch Reactors Dealing with Reject Water Engineering Transactions PCR-DGGE SBR bacterial community monitoring reject water |
| title | PCR-DGGE Based Biodiveristy, Changeability and Genetic Similarity Analysis of Bacterial Community in Sequencing Batch Reactors Dealing with Reject Water |
| title_full | PCR-DGGE Based Biodiveristy, Changeability and Genetic Similarity Analysis of Bacterial Community in Sequencing Batch Reactors Dealing with Reject Water |
| title_fullStr | PCR-DGGE Based Biodiveristy, Changeability and Genetic Similarity Analysis of Bacterial Community in Sequencing Batch Reactors Dealing with Reject Water |
| title_full_unstemmed | PCR-DGGE Based Biodiveristy, Changeability and Genetic Similarity Analysis of Bacterial Community in Sequencing Batch Reactors Dealing with Reject Water |
| title_short | PCR-DGGE Based Biodiveristy, Changeability and Genetic Similarity Analysis of Bacterial Community in Sequencing Batch Reactors Dealing with Reject Water |
| title_sort | pcr dgge based biodiveristy changeability and genetic similarity analysis of bacterial community in sequencing batch reactors dealing with reject water |
| topic | PCR-DGGE SBR bacterial community monitoring reject water |
| url | https://et.ippt.pan.pl/index.php/et/article/view/35 |
| work_keys_str_mv | AT aleksandraziembinskabuczynska pcrdggebasedbiodiveristychangeabilityandgeneticsimilarityanalysisofbacterialcommunityinsequencingbatchreactorsdealingwithrejectwater AT grzegorzcema pcrdggebasedbiodiveristychangeabilityandgeneticsimilarityanalysisofbacterialcommunityinsequencingbatchreactorsdealingwithrejectwater AT martaszpindor pcrdggebasedbiodiveristychangeabilityandgeneticsimilarityanalysisofbacterialcommunityinsequencingbatchreactorsdealingwithrejectwater AT annameresta pcrdggebasedbiodiveristychangeabilityandgeneticsimilarityanalysisofbacterialcommunityinsequencingbatchreactorsdealingwithrejectwater AT lesławpłonka pcrdggebasedbiodiveristychangeabilityandgeneticsimilarityanalysisofbacterialcommunityinsequencingbatchreactorsdealingwithrejectwater |