Development and validation of a RP–HPLC method for the quantitation studies of fipronil in Parakill suspensions
An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of fipronil in suspensions of Parakill. HPLC separation was carried out by reversed phase chromatography on Betasil C18 (250 mm x 4.6 mm i.d.; 5 μm particle size), held in thermostat...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Asociaţia Naţională a Fabricanţilor de Produse de Uz Veterinar
2011-12-01
|
| Series: | Medicamentul Veterinar |
| Subjects: | |
| Online Access: | http://www.veterinarypharmacon.com/docs/993-2011-ART.%207.%20eng.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of fipronil in suspensions of Parakill. HPLC separation was carried out by reversed phase chromatography on Betasil C18 (250 mm x 4.6 mm i.d.; 5 μm particle size), held in thermostat at 25°C. The mobile phase consisted of Acetonitrile/ Distilled water (60/ 40 v/ v), with a flow rate of 1 ml/ min and with UV detection at 220 nm. In order to validate the method, the following parameters have been investigated linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of the active pharmaceutical compound in PARAKILL suspensions. |
|---|---|
| ISSN: | 1843-9527 2069-2463 |