CHANGE IN CONTENT OF GLYCOPROTEINS ON THE SURFACE OF ENDOTHELIAL CELL CULTURE EA.HY 926 AND INTIMA OF INTERNAL CAROTID ARTERIES UNDER THE INFLUENCE OF MAGNESIUM OROTATE
Aim. To study changes in the glycoproteins content in the glycocalyx of endothelial cells under the influence of magnesium orotate.Material and methods. Endotheliocytes of cell culture EA.hy 926 were examined before and after addition of the magnesium orotate to the culture medium. Intimal endothel...
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Столичная издательская компания
2017-03-01
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| Series: | Рациональная фармакотерапия в кардиологии |
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| Online Access: | https://www.rpcardio.online/jour/article/view/1421 |
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| author | L. V. Didenko E. A. Ulubieva T. G. Borovaya A. G. Avtandilov N. V. Shevlyagina V. G. Zhukhovitsky O. M. Grinkevich M. V. Mezentseva |
| author_facet | L. V. Didenko E. A. Ulubieva T. G. Borovaya A. G. Avtandilov N. V. Shevlyagina V. G. Zhukhovitsky O. M. Grinkevich M. V. Mezentseva |
| author_sort | L. V. Didenko |
| collection | DOAJ |
| description | Aim. To study changes in the glycoproteins content in the glycocalyx of endothelial cells under the influence of magnesium orotate.Material and methods. Endotheliocytes of cell culture EA.hy 926 were examined before and after addition of the magnesium orotate to the culture medium. Intimal endothelium of internal carotid arteries, fragments of which were obtained by sequential execution of bilateral resection of the arteries before and after course of treatment with magnesium orotate was also investigated. Anthony’ method (1931), designed for the study of glycoproteins in the bacteria capsule and adapted by L.V. Didenko (2013) for eukaryotes was used to detect glycoproteins. The scanning electron microscopy with simultaneous x-ray microanalysis was applied.Results. Total number of Cu2+-signals per one scanning field in the mapping of samples for Cu2+ as the indirect index of proteoglycan content in EA.hy 926 endothelial cell culture before incubation with magnesium orotate was 6928±124, and after incubation with magnesium orotate – 7592±131. Number of Cu2+-signals on the surface of the internal carotid artery intima before treatment with magnesium orotate was 5015±407, and after treatment – 6100±152 per one scanning field.Conclusion. A significant increase in the content of glycoproteins on the surface of EA.hy926 endothelial cell culture (+10%) and internal carotid artery intima (+22%) under the influence of magnesium orotate was found. |
| format | Article |
| id | doaj-art-f19a957f226f4d18af26ef873f886e15 |
| institution | Matheson Library |
| issn | 1819-6446 2225-3653 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | Столичная издательская компания |
| record_format | Article |
| series | Рациональная фармакотерапия в кардиологии |
| spelling | doaj-art-f19a957f226f4d18af26ef873f886e152025-07-03T07:28:32ZengСтоличная издательская компанияРациональная фармакотерапия в кардиологии1819-64462225-36532017-03-01131889410.20996/1819-6446-2017-13-1-88-941310CHANGE IN CONTENT OF GLYCOPROTEINS ON THE SURFACE OF ENDOTHELIAL CELL CULTURE EA.HY 926 AND INTIMA OF INTERNAL CAROTID ARTERIES UNDER THE INFLUENCE OF MAGNESIUM OROTATEL. V. Didenko0E. A. Ulubieva1T. G. Borovaya2A. G. Avtandilov3N. V. Shevlyagina4V. G. Zhukhovitsky5O. M. Grinkevich6M. V. Mezentseva7Gamaleya Federal Research Center for Epidemiology and MicrobiologyRussian Medical Academy of Postgraduate EducationGamaleya Federal Research Center for Epidemiology and MicrobiologyRussian Medical Academy of Postgraduate EducationGamaleya Federal Research Center for Epidemiology and MicrobiologyGamaleya Federal Research Center for Epidemiology and MicrobiologyGamaleya Federal Research Center for Epidemiology and MicrobiologyGamaleya Federal Research Center for Epidemiology and MicrobiologyAim. To study changes in the glycoproteins content in the glycocalyx of endothelial cells under the influence of magnesium orotate.Material and methods. Endotheliocytes of cell culture EA.hy 926 were examined before and after addition of the magnesium orotate to the culture medium. Intimal endothelium of internal carotid arteries, fragments of which were obtained by sequential execution of bilateral resection of the arteries before and after course of treatment with magnesium orotate was also investigated. Anthony’ method (1931), designed for the study of glycoproteins in the bacteria capsule and adapted by L.V. Didenko (2013) for eukaryotes was used to detect glycoproteins. The scanning electron microscopy with simultaneous x-ray microanalysis was applied.Results. Total number of Cu2+-signals per one scanning field in the mapping of samples for Cu2+ as the indirect index of proteoglycan content in EA.hy 926 endothelial cell culture before incubation with magnesium orotate was 6928±124, and after incubation with magnesium orotate – 7592±131. Number of Cu2+-signals on the surface of the internal carotid artery intima before treatment with magnesium orotate was 5015±407, and after treatment – 6100±152 per one scanning field.Conclusion. A significant increase in the content of glycoproteins on the surface of EA.hy926 endothelial cell culture (+10%) and internal carotid artery intima (+22%) under the influence of magnesium orotate was found.https://www.rpcardio.online/jour/article/view/1421electron microscopyendothelial cellsglycoproteinsglycocalyxcopper sulfatemagnesium orotate |
| spellingShingle | L. V. Didenko E. A. Ulubieva T. G. Borovaya A. G. Avtandilov N. V. Shevlyagina V. G. Zhukhovitsky O. M. Grinkevich M. V. Mezentseva CHANGE IN CONTENT OF GLYCOPROTEINS ON THE SURFACE OF ENDOTHELIAL CELL CULTURE EA.HY 926 AND INTIMA OF INTERNAL CAROTID ARTERIES UNDER THE INFLUENCE OF MAGNESIUM OROTATE Рациональная фармакотерапия в кардиологии electron microscopy endothelial cells glycoproteins glycocalyx copper sulfate magnesium orotate |
| title | CHANGE IN CONTENT OF GLYCOPROTEINS ON THE SURFACE OF ENDOTHELIAL CELL CULTURE EA.HY 926 AND INTIMA OF INTERNAL CAROTID ARTERIES UNDER THE INFLUENCE OF MAGNESIUM OROTATE |
| title_full | CHANGE IN CONTENT OF GLYCOPROTEINS ON THE SURFACE OF ENDOTHELIAL CELL CULTURE EA.HY 926 AND INTIMA OF INTERNAL CAROTID ARTERIES UNDER THE INFLUENCE OF MAGNESIUM OROTATE |
| title_fullStr | CHANGE IN CONTENT OF GLYCOPROTEINS ON THE SURFACE OF ENDOTHELIAL CELL CULTURE EA.HY 926 AND INTIMA OF INTERNAL CAROTID ARTERIES UNDER THE INFLUENCE OF MAGNESIUM OROTATE |
| title_full_unstemmed | CHANGE IN CONTENT OF GLYCOPROTEINS ON THE SURFACE OF ENDOTHELIAL CELL CULTURE EA.HY 926 AND INTIMA OF INTERNAL CAROTID ARTERIES UNDER THE INFLUENCE OF MAGNESIUM OROTATE |
| title_short | CHANGE IN CONTENT OF GLYCOPROTEINS ON THE SURFACE OF ENDOTHELIAL CELL CULTURE EA.HY 926 AND INTIMA OF INTERNAL CAROTID ARTERIES UNDER THE INFLUENCE OF MAGNESIUM OROTATE |
| title_sort | change in content of glycoproteins on the surface of endothelial cell culture ea hy 926 and intima of internal carotid arteries under the influence of magnesium orotate |
| topic | electron microscopy endothelial cells glycoproteins glycocalyx copper sulfate magnesium orotate |
| url | https://www.rpcardio.online/jour/article/view/1421 |
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