Recombination of mannanase gene man23 and its expression in Brevibacillus brevis
To promote the production of mannanase (Man23) and reduce its degradation in vitro, the man23 gene was ligated with a high-expression vector pHY-p43. The recombinant plasmid pHY-p43-man23 was transformed into Brevibacillus brevis. The man23 gene regulated by the strong promoter p43 produced mannanas...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2008-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.04.007 |
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| author | ZHOU Hai-yan RAO Li-qun WU Yong-yao |
| author_facet | ZHOU Hai-yan RAO Li-qun WU Yong-yao |
| author_sort | ZHOU Hai-yan |
| collection | DOAJ |
| description | To promote the production of mannanase (Man23) and reduce its degradation in vitro, the man23 gene was ligated with a high-expression vector pHY-p43. The recombinant plasmid pHY-p43-man23 was transformed into Brevibacillus brevis. The man23 gene regulated by the strong promoter p43 produced mannanase activity of 22480 U·mL<sup>-1</sup>, which was 26.7% higher than original strain Bacillus subtilis B23. Because B. brevis is a protease-defective strain, SDS-PAGE of the extracellular protein from B. brevis showed a much stronger band of the target protein and week or little non-target protein bands. This efficient and economic system can produce correctly folded extracellular mannanase with much higher yield and activity. The expression profile indicated that B. brevis was a perfect host cell for gene engineering. |
| format | Article |
| id | doaj-art-f15205f1c83c44f8adc53ee7fea4cc57 |
| institution | Matheson Library |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2008-07-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-f15205f1c83c44f8adc53ee7fea4cc572025-08-01T05:36:34ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552008-07-013438939410.3785/j.issn.1008-9209.2008.04.00710089209Recombination of mannanase gene man23 and its expression in Brevibacillus brevisZHOU Hai-yanRAO Li-qunWU Yong-yaoTo promote the production of mannanase (Man23) and reduce its degradation in vitro, the man23 gene was ligated with a high-expression vector pHY-p43. The recombinant plasmid pHY-p43-man23 was transformed into Brevibacillus brevis. The man23 gene regulated by the strong promoter p43 produced mannanase activity of 22480 U·mL<sup>-1</sup>, which was 26.7% higher than original strain Bacillus subtilis B23. Because B. brevis is a protease-defective strain, SDS-PAGE of the extracellular protein from B. brevis showed a much stronger band of the target protein and week or little non-target protein bands. This efficient and economic system can produce correctly folded extracellular mannanase with much higher yield and activity. The expression profile indicated that B. brevis was a perfect host cell for gene engineering.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.04.007mannanase<italic>Brevibacillus brevis</italic>p43 promoterhigh-level expression |
| spellingShingle | ZHOU Hai-yan RAO Li-qun WU Yong-yao Recombination of mannanase gene man23 and its expression in Brevibacillus brevis 浙江大学学报. 农业与生命科学版 mannanase <italic>Brevibacillus brevis</italic> p43 promoter high-level expression |
| title | Recombination of mannanase gene man23 and its expression in Brevibacillus brevis |
| title_full | Recombination of mannanase gene man23 and its expression in Brevibacillus brevis |
| title_fullStr | Recombination of mannanase gene man23 and its expression in Brevibacillus brevis |
| title_full_unstemmed | Recombination of mannanase gene man23 and its expression in Brevibacillus brevis |
| title_short | Recombination of mannanase gene man23 and its expression in Brevibacillus brevis |
| title_sort | recombination of mannanase gene man23 and its expression in brevibacillus brevis |
| topic | mannanase <italic>Brevibacillus brevis</italic> p43 promoter high-level expression |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.04.007 |
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