Recombination of mannanase gene man23 and its expression in Brevibacillus brevis
To promote the production of mannanase (Man23) and reduce its degradation in vitro, the man23 gene was ligated with a high-expression vector pHY-p43. The recombinant plasmid pHY-p43-man23 was transformed into Brevibacillus brevis. The man23 gene regulated by the strong promoter p43 produced mannanas...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2008-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.04.007 |
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| Summary: | To promote the production of mannanase (Man23) and reduce its degradation in vitro, the man23 gene was ligated with a high-expression vector pHY-p43. The recombinant plasmid pHY-p43-man23 was transformed into Brevibacillus brevis. The man23 gene regulated by the strong promoter p43 produced mannanase activity of 22480 U·mL<sup>-1</sup>, which was 26.7% higher than original strain Bacillus subtilis B23. Because B. brevis is a protease-defective strain, SDS-PAGE of the extracellular protein from B. brevis showed a much stronger band of the target protein and week or little non-target protein bands. This efficient and economic system can produce correctly folded extracellular mannanase with much higher yield and activity. The expression profile indicated that B. brevis was a perfect host cell for gene engineering. |
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| ISSN: | 1008-9209 2097-5155 |