A plastidic ATP/ADP transporter gene, IbAATP, increases starch and amylose contents and alters starch structure in transgenic sweetpotato
A plastidic adenosine triphosphate (ATP)/adenosine diphosphate (ADP) transporter (AATP) is responsible for importing ATP from the cytosol into plastids. In dicotyledonous plants, increasing ATP supply is a potential way to facilitate anabolic synthesis in heterotrophic plastids. In this study, a gen...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2016-09-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915611923 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839595372058509312 |
|---|---|
| author | Yan-nan WANG Yan LI Huan ZHANG Hong ZHAI Qing-chang LIU Shao-zhen HE |
| author_facet | Yan-nan WANG Yan LI Huan ZHANG Hong ZHAI Qing-chang LIU Shao-zhen HE |
| author_sort | Yan-nan WANG |
| collection | DOAJ |
| description | A plastidic adenosine triphosphate (ATP)/adenosine diphosphate (ADP) transporter (AATP) is responsible for importing ATP from the cytosol into plastids. In dicotyledonous plants, increasing ATP supply is a potential way to facilitate anabolic synthesis in heterotrophic plastids. In this study, a gene encoding the AATP protein, named IbAATP, was isolated from sweetpotato (Ipomoea batatas (L.) Lam.). Transcripts of IbAATP were predominantly detected in the storage roots and leaves and were induced by exogenous sucrose and subjected to circadian rhythm. Transient expression of IbAATP in tobacco and onion epidermal cells revealed the plastidic localization of IbAATP. The overexpression of IbAATP in sweetpotato significantly increased the starch and amylose contents and led to enlarged starch granules. The IbAATP-overexpressing plants showed altered fine structure of amylopectin, which contained an increased proportion of chains with a degree of polymerization (DP) of 10–23 and a reduced number of chains with a DP of 5–9 and 24–40. In addition, starch from the transgenic plants exhibited different pasting properties. The transcript levels of starch biosynthetic genes, including IbAGP, IbGBSSI, IbSSI-IV, and IbSBE, were differentially regulated in the transgenic plants. These results revealed the explicit role of IbAATP in the starch biosynthesis of sweetpotato and indicated that this gene has the potential to be used to improve starch content and quality in sweetpotato and other plants. |
| format | Article |
| id | doaj-art-f04fb9525e3e4311af83d3e6660fd79c |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-f04fb9525e3e4311af83d3e6660fd79c2025-08-02T23:03:39ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192016-09-0115919681982A plastidic ATP/ADP transporter gene, IbAATP, increases starch and amylose contents and alters starch structure in transgenic sweetpotatoYan-nan WANG0Yan LI1Huan ZHANG2Hong ZHAI3Qing-chang LIU4Shao-zhen HE5WANG Yan-nan, +86-10-62732559; College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, P.R.ChinaCollege of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, P.R.ChinaCollege of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, P.R.ChinaCollege of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, P.R.ChinaCollege of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, P.R.ChinaCorrespondence HE Shao-zhen, +86-10-62732559; College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, P.R.ChinaA plastidic adenosine triphosphate (ATP)/adenosine diphosphate (ADP) transporter (AATP) is responsible for importing ATP from the cytosol into plastids. In dicotyledonous plants, increasing ATP supply is a potential way to facilitate anabolic synthesis in heterotrophic plastids. In this study, a gene encoding the AATP protein, named IbAATP, was isolated from sweetpotato (Ipomoea batatas (L.) Lam.). Transcripts of IbAATP were predominantly detected in the storage roots and leaves and were induced by exogenous sucrose and subjected to circadian rhythm. Transient expression of IbAATP in tobacco and onion epidermal cells revealed the plastidic localization of IbAATP. The overexpression of IbAATP in sweetpotato significantly increased the starch and amylose contents and led to enlarged starch granules. The IbAATP-overexpressing plants showed altered fine structure of amylopectin, which contained an increased proportion of chains with a degree of polymerization (DP) of 10–23 and a reduced number of chains with a DP of 5–9 and 24–40. In addition, starch from the transgenic plants exhibited different pasting properties. The transcript levels of starch biosynthetic genes, including IbAGP, IbGBSSI, IbSSI-IV, and IbSBE, were differentially regulated in the transgenic plants. These results revealed the explicit role of IbAATP in the starch biosynthesis of sweetpotato and indicated that this gene has the potential to be used to improve starch content and quality in sweetpotato and other plants.http://www.sciencedirect.com/science/article/pii/S2095311915611923sweetpotatoIbAATPstarch content and compositionstarch granule sizestarch structurepasting properties |
| spellingShingle | Yan-nan WANG Yan LI Huan ZHANG Hong ZHAI Qing-chang LIU Shao-zhen HE A plastidic ATP/ADP transporter gene, IbAATP, increases starch and amylose contents and alters starch structure in transgenic sweetpotato Journal of Integrative Agriculture sweetpotato IbAATP starch content and composition starch granule size starch structure pasting properties |
| title | A plastidic ATP/ADP transporter gene, IbAATP, increases starch and amylose contents and alters starch structure in transgenic sweetpotato |
| title_full | A plastidic ATP/ADP transporter gene, IbAATP, increases starch and amylose contents and alters starch structure in transgenic sweetpotato |
| title_fullStr | A plastidic ATP/ADP transporter gene, IbAATP, increases starch and amylose contents and alters starch structure in transgenic sweetpotato |
| title_full_unstemmed | A plastidic ATP/ADP transporter gene, IbAATP, increases starch and amylose contents and alters starch structure in transgenic sweetpotato |
| title_short | A plastidic ATP/ADP transporter gene, IbAATP, increases starch and amylose contents and alters starch structure in transgenic sweetpotato |
| title_sort | plastidic atp adp transporter gene ibaatp increases starch and amylose contents and alters starch structure in transgenic sweetpotato |
| topic | sweetpotato IbAATP starch content and composition starch granule size starch structure pasting properties |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915611923 |
| work_keys_str_mv | AT yannanwang aplastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato AT yanli aplastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato AT huanzhang aplastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato AT hongzhai aplastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato AT qingchangliu aplastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato AT shaozhenhe aplastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato AT yannanwang plastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato AT yanli plastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato AT huanzhang plastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato AT hongzhai plastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato AT qingchangliu plastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato AT shaozhenhe plastidicatpadptransportergeneibaatpincreasesstarchandamylosecontentsandaltersstarchstructureintransgenicsweetpotato |