Determination of morphology and immunophenotype of circulating lymphoma cells in patients with splenic marginal zone lymphoma using an anti-CD antibody microarray
We have studied the morphology and immunophenotype of circulating tumor cells isolated from peripheral blood of 22 patients with splenic marginal zone lymphoma and show that both of them are highly heterogeneous. Using a cell-binding microarray we have demonstrated that the circulating lymphoma cell...
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| Format: | Article |
| Language: | Russian |
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ABV-press
2017-04-01
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| Series: | Онкогематология |
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| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/233 |
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| author | A. N. Khvastunova L. S. Al-Radi O. S. Fedyanina U. L. Julhakyan N. M. Kapranov A. O. Zakirova S. A. Lugovskaya E. V. Naumova F. I. Ataullakhanov S. A. Kuznetsova |
| author_facet | A. N. Khvastunova L. S. Al-Radi O. S. Fedyanina U. L. Julhakyan N. M. Kapranov A. O. Zakirova S. A. Lugovskaya E. V. Naumova F. I. Ataullakhanov S. A. Kuznetsova |
| author_sort | A. N. Khvastunova |
| collection | DOAJ |
| description | We have studied the morphology and immunophenotype of circulating tumor cells isolated from peripheral blood of 22 patients with splenic marginal zone lymphoma and show that both of them are highly heterogeneous. Using a cell-binding microarray we have demonstrated that the circulating lymphoma cells are positive for CD19 (100 %), CD20 (100 %), CD22 (100 %), surface IgM (73 %), CD38 (23 %), CD5 (9 %), CD11c (36 %), CD103 (5 %), CD25 (32 %), CD23 (23 %) and these immunophenotypes are confirmed in all cases by flow cytometry.Higher surface density of lymphocyte binding onto anti-CD antibody microarray spots compared to blood smears permits to find circulating lymphoma cells even in leukopenic patients. |
| format | Article |
| id | doaj-art-f00e7a1104a640b0b3d7c6086e6ac13c |
| institution | Matheson Library |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| publishDate | 2017-04-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj-art-f00e7a1104a640b0b3d7c6086e6ac13c2025-08-04T13:57:49ZrusABV-pressОнкогематология1818-83462413-40232017-04-01121717710.17650/1818-8346-2017-12-1-71-77231Determination of morphology and immunophenotype of circulating lymphoma cells in patients with splenic marginal zone lymphoma using an anti-CD antibody microarrayA. N. Khvastunova0L. S. Al-Radi1O. S. Fedyanina2U. L. Julhakyan3N. M. Kapranov4A. O. Zakirova5S. A. Lugovskaya6E. V. Naumova7F. I. Ataullakhanov8S. A. Kuznetsova9National Scientific and Practical Center of Pediatric Hematology, Oncology and Immunnology named after Dmitry Rogachev, Ministry of Health of Russia; Theoretical Problems Center of Physical and Chemical Pharmacology RAS;Hematological Research Center;National Scientific and Practical Center of Pediatric Hematology, Oncology and Immunnology named after Dmitry Rogachev, Ministry of Health of Russia; Theoretical Problems Center of Physical and Chemical Pharmacology RAS;Hematological Research Center;Theoretical Problems Center of Physical and Chemical Pharmacology RAS; Hematological Research Center;National Scientific and Practical Center of Pediatric Hematology, Oncology and Immunnology named after Dmitry Rogachev, Ministry of Health of Russia; Theoretical Problems Center of Physical and Chemical Pharmacology RAS;Russian Medical Academy of Continuous Professional Education, Ministry of Health of Russia;Russian Medical Academy of Continuous Professional Education, Ministry of Health of Russia;National Scientific and Practical Center of Pediatric Hematology, Oncology and Immunnology named after Dmitry Rogachev, Ministry of Health of Russia; Theoretical Problems Center of Physical and Chemical Pharmacology RAS;National Scientific and Practical Center of Pediatric Hematology, Oncology and Immunnology named after Dmitry Rogachev, Ministry of Health of Russia; Theoretical Problems Center of Physical and Chemical Pharmacology RAS;We have studied the morphology and immunophenotype of circulating tumor cells isolated from peripheral blood of 22 patients with splenic marginal zone lymphoma and show that both of them are highly heterogeneous. Using a cell-binding microarray we have demonstrated that the circulating lymphoma cells are positive for CD19 (100 %), CD20 (100 %), CD22 (100 %), surface IgM (73 %), CD38 (23 %), CD5 (9 %), CD11c (36 %), CD103 (5 %), CD25 (32 %), CD23 (23 %) and these immunophenotypes are confirmed in all cases by flow cytometry.Higher surface density of lymphocyte binding onto anti-CD antibody microarray spots compared to blood smears permits to find circulating lymphoma cells even in leukopenic patients.https://oncohematology.abvpress.ru/ongm/article/view/233anti-cd antibody microarraysplenic marginal zone lymphomamorphologyimmunophenotypecd5cd11ccd23cd25cd103 |
| spellingShingle | A. N. Khvastunova L. S. Al-Radi O. S. Fedyanina U. L. Julhakyan N. M. Kapranov A. O. Zakirova S. A. Lugovskaya E. V. Naumova F. I. Ataullakhanov S. A. Kuznetsova Determination of morphology and immunophenotype of circulating lymphoma cells in patients with splenic marginal zone lymphoma using an anti-CD antibody microarray Онкогематология anti-cd antibody microarray splenic marginal zone lymphoma morphology immunophenotype cd5 cd11c cd23 cd25 cd103 |
| title | Determination of morphology and immunophenotype of circulating lymphoma cells in patients with splenic marginal zone lymphoma using an anti-CD antibody microarray |
| title_full | Determination of morphology and immunophenotype of circulating lymphoma cells in patients with splenic marginal zone lymphoma using an anti-CD antibody microarray |
| title_fullStr | Determination of morphology and immunophenotype of circulating lymphoma cells in patients with splenic marginal zone lymphoma using an anti-CD antibody microarray |
| title_full_unstemmed | Determination of morphology and immunophenotype of circulating lymphoma cells in patients with splenic marginal zone lymphoma using an anti-CD antibody microarray |
| title_short | Determination of morphology and immunophenotype of circulating lymphoma cells in patients with splenic marginal zone lymphoma using an anti-CD antibody microarray |
| title_sort | determination of morphology and immunophenotype of circulating lymphoma cells in patients with splenic marginal zone lymphoma using an anti cd antibody microarray |
| topic | anti-cd antibody microarray splenic marginal zone lymphoma morphology immunophenotype cd5 cd11c cd23 cd25 cd103 |
| url | https://oncohematology.abvpress.ru/ongm/article/view/233 |
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