The Effects of Storage Conditions and Homogenisation Buffers on the Measurement of SOD, CAT and ADA Enzyme Activities in Cattle Liver
ABSTRACT Background Accurate measurement of enzyme activities is very important in studies to evaluate enzymatic parameters. While performing these measurements, many factors can affect the results, including the method of obtaining the tissues, the conditions under which they are stored until analy...
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Wiley
2025-07-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70418 |
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| author | Burcu Menekse Balkan Ogunc Meral Bekir Cetintav Hidayet Tutun Guzin Ozkurt Tevhide Sel |
| author_facet | Burcu Menekse Balkan Ogunc Meral Bekir Cetintav Hidayet Tutun Guzin Ozkurt Tevhide Sel |
| author_sort | Burcu Menekse Balkan |
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| description | ABSTRACT Background Accurate measurement of enzyme activities is very important in studies to evaluate enzymatic parameters. While performing these measurements, many factors can affect the results, including the method of obtaining the tissues, the conditions under which they are stored until analysis, and the methods of determining enzyme activity. Objectives This study aimed to investigate the effect of different storage conditions (time and temperature) and different homogenisation buffers (PBS or KCl) on the enzymatic activity of superoxide dismutase (SOD), catalase (CAT), and adenosine deaminase (ADA) in homogenised cattle liver. Methods Fresh liver tissue samples were obtained from the slaughterhouse and homogenised in different homogenisation buffers. Supernatants from each sample were divided into three groups according to the experimental design of the study. SOD, CAT and ADA enzyme activities in homogenised tissues were evaluated. Results Our data revealed that SOD, CAT and ADA activities did not differ significantly between PBS and KCl groups or between ‐20°C and ‐80°C freezing temperatures. However, our results showed that SOD levels decreased over time in both groups, CAT levels demonstrated a significant decrease from Month 0 to Month 3 and ADA levels decreased significantly over time. Conclusions The results indicated that storage time had a significant effect on enzyme activity changes, but the effect of storage temperature and homogenisation buffer was generally limited. These results may support the measurement of enzymatic activity in liver homogenate immediately and, if necessary, after freezing for the shortest possible time. |
| format | Article |
| id | doaj-art-efe709a35e0e44d5864d25c1f81eddfb |
| institution | Matheson Library |
| issn | 2053-1095 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
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| series | Veterinary Medicine and Science |
| spelling | doaj-art-efe709a35e0e44d5864d25c1f81eddfb2025-07-22T14:05:52ZengWileyVeterinary Medicine and Science2053-10952025-07-01114n/an/a10.1002/vms3.70418The Effects of Storage Conditions and Homogenisation Buffers on the Measurement of SOD, CAT and ADA Enzyme Activities in Cattle LiverBurcu Menekse Balkan0Ogunc Meral1Bekir Cetintav2Hidayet Tutun3Guzin Ozkurt4Tevhide Sel5Department of Biochemistry Faculty of Veterinary Medicine Burdur Mehmet Akif Ersoy University Burdur TurkeyDepartment of Biochemistry Faculty of Veterinary Medicine Ankara University Ankara TurkeyDepartment of Biostatistics Faculty of Veterinary Medicine Burdur Mehmet Akif Ersoy University Burdur TurkeyDepartment of Pharmacology and Toxicology Faculty of Veterinary Medicine Burdur Mehmet Akif Ersoy University Burdur TurkeyDepartment of Biochemistry Faculty of Veterinary Medicine Aksaray University Aksaray TurkeyDepartment of Biochemistry Faculty of Veterinary Medicine Ankara University Ankara TurkeyABSTRACT Background Accurate measurement of enzyme activities is very important in studies to evaluate enzymatic parameters. While performing these measurements, many factors can affect the results, including the method of obtaining the tissues, the conditions under which they are stored until analysis, and the methods of determining enzyme activity. Objectives This study aimed to investigate the effect of different storage conditions (time and temperature) and different homogenisation buffers (PBS or KCl) on the enzymatic activity of superoxide dismutase (SOD), catalase (CAT), and adenosine deaminase (ADA) in homogenised cattle liver. Methods Fresh liver tissue samples were obtained from the slaughterhouse and homogenised in different homogenisation buffers. Supernatants from each sample were divided into three groups according to the experimental design of the study. SOD, CAT and ADA enzyme activities in homogenised tissues were evaluated. Results Our data revealed that SOD, CAT and ADA activities did not differ significantly between PBS and KCl groups or between ‐20°C and ‐80°C freezing temperatures. However, our results showed that SOD levels decreased over time in both groups, CAT levels demonstrated a significant decrease from Month 0 to Month 3 and ADA levels decreased significantly over time. Conclusions The results indicated that storage time had a significant effect on enzyme activity changes, but the effect of storage temperature and homogenisation buffer was generally limited. These results may support the measurement of enzymatic activity in liver homogenate immediately and, if necessary, after freezing for the shortest possible time.https://doi.org/10.1002/vms3.70418adenosine deaminasecatalasecattleenzymatic activityliversuperoxide dismutase |
| spellingShingle | Burcu Menekse Balkan Ogunc Meral Bekir Cetintav Hidayet Tutun Guzin Ozkurt Tevhide Sel The Effects of Storage Conditions and Homogenisation Buffers on the Measurement of SOD, CAT and ADA Enzyme Activities in Cattle Liver Veterinary Medicine and Science adenosine deaminase catalase cattle enzymatic activity liver superoxide dismutase |
| title | The Effects of Storage Conditions and Homogenisation Buffers on the Measurement of SOD, CAT and ADA Enzyme Activities in Cattle Liver |
| title_full | The Effects of Storage Conditions and Homogenisation Buffers on the Measurement of SOD, CAT and ADA Enzyme Activities in Cattle Liver |
| title_fullStr | The Effects of Storage Conditions and Homogenisation Buffers on the Measurement of SOD, CAT and ADA Enzyme Activities in Cattle Liver |
| title_full_unstemmed | The Effects of Storage Conditions and Homogenisation Buffers on the Measurement of SOD, CAT and ADA Enzyme Activities in Cattle Liver |
| title_short | The Effects of Storage Conditions and Homogenisation Buffers on the Measurement of SOD, CAT and ADA Enzyme Activities in Cattle Liver |
| title_sort | effects of storage conditions and homogenisation buffers on the measurement of sod cat and ada enzyme activities in cattle liver |
| topic | adenosine deaminase catalase cattle enzymatic activity liver superoxide dismutase |
| url | https://doi.org/10.1002/vms3.70418 |
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