Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine
Non-expressor of pathogenesis-related genes 1 (NPR1) plays a significant role in the defense responses of plants to pathogens by regulating the expression of defense-related genes. In the present study, we isolated two NPR1 genes from Vitis aestivalis cv. Norton and Vitis vinifera cv. Cabernet Sauvi...
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KeAi Communications Co., Ltd.
2013-07-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311913604323 |
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| author | Yi-ming ZHANG Xi-lu NI Hui-qin MA Wenping Qiu |
| author_facet | Yi-ming ZHANG Xi-lu NI Hui-qin MA Wenping Qiu |
| author_sort | Yi-ming ZHANG |
| collection | DOAJ |
| description | Non-expressor of pathogenesis-related genes 1 (NPR1) plays a significant role in the defense responses of plants to pathogens by regulating the expression of defense-related genes. In the present study, we isolated two NPR1 genes from Vitis aestivalis cv. Norton and Vitis vinifera cv. Cabernet Sauvignon, which were referred to as VaNPR1.1 and VvNPR1. 1-CS, respectively. They encode a protein of 584 amino acids with a predicted molecular weight of 64.8 kDa and a theoretical isoelectric point (pI) of 5.74. The predicted amino acid sequences of VaNPR1.1 and VvNPR1.1-CS differ by only one amino acid. Over-expression of VaNPR1.1 gene in Arabidopsis npr1-1 mutant plants restores the transcriptional expression of AtPR-1 gene, though not to the full scale. This result demonstrated that a grapevine VaNPR1.1 possesses a similar function to the Arabidopsis NPR1 in the regulation of defense-related genes. Over-expression of VaNPR1.1 in transgenic Arabidopsis plant increased tolerance to salinity, but had no effect on the drought tolerance. We conclude that VaNPR1.1 is a functional ortholog of AtNPR1 and also involved in grapevine's response to the salt stress. |
| format | Article |
| id | doaj-art-ecf4be74b8a94404836c46e8d15b0b4d |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2013-07-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Journal of Integrative Agriculture |
| spelling | doaj-art-ecf4be74b8a94404836c46e8d15b0b4d2025-08-02T22:44:16ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192013-07-0112711521161Characterization of NPR1 Genes from Norton and Cabernet Sauvignon GrapevineYi-ming ZHANG0Xi-lu NI1Hui-qin MA2Wenping Qiu3Department of Fruit Tree Sciences, College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P. R. China; The Center for Grapevine Biotechnology, William H. Darr School of Agriculture, Missouri State University, Mountain Grove, MO 65711, USA; ZHANG Yi-ming, Mobile: 15041248655Ningxia Forestry Institute, State Key Laboratory of Seedling Bioengineering, Yinchuan 750004, P.R. China; College of Life Science, Northwest University, Xi'an 710069, P.R. ChinaDepartment of Fruit Tree Sciences, College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P. R. China; Correspondence MA Hui-qinThe Center for Grapevine Biotechnology, William H. Darr School of Agriculture, Missouri State University, Mountain Grove, MO 65711, USANon-expressor of pathogenesis-related genes 1 (NPR1) plays a significant role in the defense responses of plants to pathogens by regulating the expression of defense-related genes. In the present study, we isolated two NPR1 genes from Vitis aestivalis cv. Norton and Vitis vinifera cv. Cabernet Sauvignon, which were referred to as VaNPR1.1 and VvNPR1. 1-CS, respectively. They encode a protein of 584 amino acids with a predicted molecular weight of 64.8 kDa and a theoretical isoelectric point (pI) of 5.74. The predicted amino acid sequences of VaNPR1.1 and VvNPR1.1-CS differ by only one amino acid. Over-expression of VaNPR1.1 gene in Arabidopsis npr1-1 mutant plants restores the transcriptional expression of AtPR-1 gene, though not to the full scale. This result demonstrated that a grapevine VaNPR1.1 possesses a similar function to the Arabidopsis NPR1 in the regulation of defense-related genes. Over-expression of VaNPR1.1 in transgenic Arabidopsis plant increased tolerance to salinity, but had no effect on the drought tolerance. We conclude that VaNPR1.1 is a functional ortholog of AtNPR1 and also involved in grapevine's response to the salt stress.http://www.sciencedirect.com/science/article/pii/S2095311913604323NPR1pathogenesis-related geneeover-expressiontransgenic Arabidopsis |
| spellingShingle | Yi-ming ZHANG Xi-lu NI Hui-qin MA Wenping Qiu Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine Journal of Integrative Agriculture NPR1 pathogenesis-related genee over-expression transgenic Arabidopsis |
| title | Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine |
| title_full | Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine |
| title_fullStr | Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine |
| title_full_unstemmed | Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine |
| title_short | Characterization of NPR1 Genes from Norton and Cabernet Sauvignon Grapevine |
| title_sort | characterization of npr1 genes from norton and cabernet sauvignon grapevine |
| topic | NPR1 pathogenesis-related genee over-expression transgenic Arabidopsis |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913604323 |
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