Harnessing <i>Streptomyces</i> for the Management of Clubroot Disease of Chinese Cabbage (<i>Brassica rapa</i> subsp. <i>Pekinensis</i>)
Clubroot, caused by <i>Plasmodiophora brassicae</i> Woronin, poses a major threat to Chinese cabbage (<i>Brassica rapa</i> subsp. <i>pekinensis</i>) production worldwide, significantly impacting crop yield, quality, and economic value. Biological control represent...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/14/2195 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839615332694622208 |
|---|---|
| author | Shan Chen Yang Zheng Qing Wang Rong Mu Xianchao Sun Guanhua Ma Liezhao Liu Jiequn Ren Kuo Huang Guokang Chen |
| author_facet | Shan Chen Yang Zheng Qing Wang Rong Mu Xianchao Sun Guanhua Ma Liezhao Liu Jiequn Ren Kuo Huang Guokang Chen |
| author_sort | Shan Chen |
| collection | DOAJ |
| description | Clubroot, caused by <i>Plasmodiophora brassicae</i> Woronin, poses a major threat to Chinese cabbage (<i>Brassica rapa</i> subsp. <i>pekinensis</i>) production worldwide, significantly impacting crop yield, quality, and economic value. Biological control represents a promising approach since it is non-toxic and eco-friendly, and it reduces the risk of pathogen resistance development. In this study, our objective was to screen for actinomycetes that can effectively inhibit clubroot. We screened 13 actinomycete strains, identifying 2, XDS3-6 and CD1-1, with substantial in vivo inhibitory effects, achieving infection suppression rates above 64% against <i>P. brassicae</i>. Phylogenetic analysis classified XDS3-6 and CD1-1 as <i>Streptomyces virginiae</i> and <i>Streptomyces cinnamonensis</i>, respectively. Both strains exhibited protease and glucanase production capabilities, essential for pathogenic suppression. Additionally, these strains induced host defense responses, as evidenced by increased jasmonic acid (JA) and salicylic acid (SA) accumulation and elevated activities of defense-related enzymes. Colonization studies of XDS3-6 and CD1-1 mutant strains in cabbage roots indicated sustained root colonization, with peak colony-forming units (CFUs) at 20 days post-inoculation, reaching 11.0 × 10<sup>4</sup> CFU/g and 8.5 × 10<sup>4</sup> CFU/g, respectively, and persisting for at least 30 days. Overall, these findings underscore the potential of <i>Streptomyces</i> strains XDS3-6 and CD1-1 as effective biocontrol agents, providing a theoretical foundation for their application in managing clubroot in Chinese cabbage. |
| format | Article |
| id | doaj-art-eb0da9be1ba84d7892f64ded78f13c52 |
| institution | Matheson Library |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-eb0da9be1ba84d7892f64ded78f13c522025-07-25T13:34:29ZengMDPI AGPlants2223-77472025-07-011414219510.3390/plants14142195Harnessing <i>Streptomyces</i> for the Management of Clubroot Disease of Chinese Cabbage (<i>Brassica rapa</i> subsp. <i>Pekinensis</i>)Shan Chen0Yang Zheng1Qing Wang2Rong Mu3Xianchao Sun4Guanhua Ma5Liezhao Liu6Jiequn Ren7Kuo Huang8Guokang Chen9Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaInstitute of Vegetable and Flower Research, Chongqing Academy of Agricultural Sciences, Chongqing 400055, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaCollege of Agronomy and Biotechnology, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, ChinaThe Chongqing Three Gorges Academy of Agricultural Sciences, Wanzhou 404155, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaClubroot, caused by <i>Plasmodiophora brassicae</i> Woronin, poses a major threat to Chinese cabbage (<i>Brassica rapa</i> subsp. <i>pekinensis</i>) production worldwide, significantly impacting crop yield, quality, and economic value. Biological control represents a promising approach since it is non-toxic and eco-friendly, and it reduces the risk of pathogen resistance development. In this study, our objective was to screen for actinomycetes that can effectively inhibit clubroot. We screened 13 actinomycete strains, identifying 2, XDS3-6 and CD1-1, with substantial in vivo inhibitory effects, achieving infection suppression rates above 64% against <i>P. brassicae</i>. Phylogenetic analysis classified XDS3-6 and CD1-1 as <i>Streptomyces virginiae</i> and <i>Streptomyces cinnamonensis</i>, respectively. Both strains exhibited protease and glucanase production capabilities, essential for pathogenic suppression. Additionally, these strains induced host defense responses, as evidenced by increased jasmonic acid (JA) and salicylic acid (SA) accumulation and elevated activities of defense-related enzymes. Colonization studies of XDS3-6 and CD1-1 mutant strains in cabbage roots indicated sustained root colonization, with peak colony-forming units (CFUs) at 20 days post-inoculation, reaching 11.0 × 10<sup>4</sup> CFU/g and 8.5 × 10<sup>4</sup> CFU/g, respectively, and persisting for at least 30 days. Overall, these findings underscore the potential of <i>Streptomyces</i> strains XDS3-6 and CD1-1 as effective biocontrol agents, providing a theoretical foundation for their application in managing clubroot in Chinese cabbage.https://www.mdpi.com/2223-7747/14/14/2195Chinese cabbagebiocontrolclubroot<i>Streptomyces</i> |
| spellingShingle | Shan Chen Yang Zheng Qing Wang Rong Mu Xianchao Sun Guanhua Ma Liezhao Liu Jiequn Ren Kuo Huang Guokang Chen Harnessing <i>Streptomyces</i> for the Management of Clubroot Disease of Chinese Cabbage (<i>Brassica rapa</i> subsp. <i>Pekinensis</i>) Plants Chinese cabbage biocontrol clubroot <i>Streptomyces</i> |
| title | Harnessing <i>Streptomyces</i> for the Management of Clubroot Disease of Chinese Cabbage (<i>Brassica rapa</i> subsp. <i>Pekinensis</i>) |
| title_full | Harnessing <i>Streptomyces</i> for the Management of Clubroot Disease of Chinese Cabbage (<i>Brassica rapa</i> subsp. <i>Pekinensis</i>) |
| title_fullStr | Harnessing <i>Streptomyces</i> for the Management of Clubroot Disease of Chinese Cabbage (<i>Brassica rapa</i> subsp. <i>Pekinensis</i>) |
| title_full_unstemmed | Harnessing <i>Streptomyces</i> for the Management of Clubroot Disease of Chinese Cabbage (<i>Brassica rapa</i> subsp. <i>Pekinensis</i>) |
| title_short | Harnessing <i>Streptomyces</i> for the Management of Clubroot Disease of Chinese Cabbage (<i>Brassica rapa</i> subsp. <i>Pekinensis</i>) |
| title_sort | harnessing i streptomyces i for the management of clubroot disease of chinese cabbage i brassica rapa i subsp i pekinensis i |
| topic | Chinese cabbage biocontrol clubroot <i>Streptomyces</i> |
| url | https://www.mdpi.com/2223-7747/14/14/2195 |
| work_keys_str_mv | AT shanchen harnessingistreptomycesiforthemanagementofclubrootdiseaseofchinesecabbageibrassicarapaisubspipekinensisi AT yangzheng harnessingistreptomycesiforthemanagementofclubrootdiseaseofchinesecabbageibrassicarapaisubspipekinensisi AT qingwang harnessingistreptomycesiforthemanagementofclubrootdiseaseofchinesecabbageibrassicarapaisubspipekinensisi AT rongmu harnessingistreptomycesiforthemanagementofclubrootdiseaseofchinesecabbageibrassicarapaisubspipekinensisi AT xianchaosun harnessingistreptomycesiforthemanagementofclubrootdiseaseofchinesecabbageibrassicarapaisubspipekinensisi AT guanhuama harnessingistreptomycesiforthemanagementofclubrootdiseaseofchinesecabbageibrassicarapaisubspipekinensisi AT liezhaoliu harnessingistreptomycesiforthemanagementofclubrootdiseaseofchinesecabbageibrassicarapaisubspipekinensisi AT jiequnren harnessingistreptomycesiforthemanagementofclubrootdiseaseofchinesecabbageibrassicarapaisubspipekinensisi AT kuohuang harnessingistreptomycesiforthemanagementofclubrootdiseaseofchinesecabbageibrassicarapaisubspipekinensisi AT guokangchen harnessingistreptomycesiforthemanagementofclubrootdiseaseofchinesecabbageibrassicarapaisubspipekinensisi |