Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum
Expressed sequence tags-derived polymerase chain reaction (EST-PCR) molecular markers specific for alien chromosomes can be used to not only monitor the introgressed alien chromatin in wheat background, but also provide the evidence of the syntenic relationship between homoeologous chromosomes. In t...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2018-08-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311917618665 |
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| author | Hao-jie SUN Jing-jing SONG Jin XIAO Tao XU Xing WEI Chun-xia YUAN Ai-zhong CAO Li-ping XING Hai-yan WANG Xiu-e WANG |
| author_facet | Hao-jie SUN Jing-jing SONG Jin XIAO Tao XU Xing WEI Chun-xia YUAN Ai-zhong CAO Li-ping XING Hai-yan WANG Xiu-e WANG |
| author_sort | Hao-jie SUN |
| collection | DOAJ |
| description | Expressed sequence tags-derived polymerase chain reaction (EST-PCR) molecular markers specific for alien chromosomes can be used to not only monitor the introgressed alien chromatin in wheat background, but also provide the evidence of the syntenic relationship between homoeologous chromosomes. In the present study, in order to develop high density and evenly distributed molecular markers specific for chromosome 6VL of Dasypyrum villosum, 297 primer pairs were designed based on the expressed sequence tags (EST) sequences, which were previously mapped in different bins of the long arms of wheat homoeologous 6AL, 6BL, and 6DL. By using the Triticum aestivum, D. villosum, T. durum-D. villosum amphiploid, and T. aestivum-D. villosum alien chromosome lines involving chromosome 6V, it was found that 32 (10.77%) primers could amplify specific bands for chromosome 6V, and 31 could be allocated to chromosome arm 6VL. These 6VL specific markers provided efficient tools for the characterization of structural variation involving the chromosome 6VL in common wheat background as well as for the selection of useful genes located on 6VL in breeding programs. |
| format | Article |
| id | doaj-art-eaf6ff7f74a24c39b82dfc9e7a85ee76 |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-eaf6ff7f74a24c39b82dfc9e7a85ee762025-08-02T22:32:48ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192018-08-0117817201726Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosumHao-jie SUN0Jing-jing SONG1Jin XIAO2Tao XU3Xing WEI4Chun-xia YUAN5Ai-zhong CAO6Li-ping XING7Hai-yan WANG8Xiu-e WANG9State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing 210095, P.R.ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing 210095, P.R.ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing 210095, P.R.ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing 210095, P.R.ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing 210095, P.R.ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing 210095, P.R.ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing 210095, P.R.ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing 210095, P.R.ChinaCorrespondence WANG Hai-yan, Tel: +86-25-84395308, Fax: +86-25-84395344; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing 210095, P.R.ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing 210095, P.R.ChinaExpressed sequence tags-derived polymerase chain reaction (EST-PCR) molecular markers specific for alien chromosomes can be used to not only monitor the introgressed alien chromatin in wheat background, but also provide the evidence of the syntenic relationship between homoeologous chromosomes. In the present study, in order to develop high density and evenly distributed molecular markers specific for chromosome 6VL of Dasypyrum villosum, 297 primer pairs were designed based on the expressed sequence tags (EST) sequences, which were previously mapped in different bins of the long arms of wheat homoeologous 6AL, 6BL, and 6DL. By using the Triticum aestivum, D. villosum, T. durum-D. villosum amphiploid, and T. aestivum-D. villosum alien chromosome lines involving chromosome 6V, it was found that 32 (10.77%) primers could amplify specific bands for chromosome 6V, and 31 could be allocated to chromosome arm 6VL. These 6VL specific markers provided efficient tools for the characterization of structural variation involving the chromosome 6VL in common wheat background as well as for the selection of useful genes located on 6VL in breeding programs.http://www.sciencedirect.com/science/article/pii/S2095311917618665Dasypyrum villosummolecular markercommon wheatalien chromosome line |
| spellingShingle | Hao-jie SUN Jing-jing SONG Jin XIAO Tao XU Xing WEI Chun-xia YUAN Ai-zhong CAO Li-ping XING Hai-yan WANG Xiu-e WANG Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum Journal of Integrative Agriculture Dasypyrum villosum molecular marker common wheat alien chromosome line |
| title | Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum |
| title_full | Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum |
| title_fullStr | Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum |
| title_full_unstemmed | Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum |
| title_short | Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum |
| title_sort | development of est pcr markers specific to the long arm of chromosome 6v of dasypyrum villosum |
| topic | Dasypyrum villosum molecular marker common wheat alien chromosome line |
| url | http://www.sciencedirect.com/science/article/pii/S2095311917618665 |
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