Comparative Proteomic Insights into the Immune Response of <i>Conogethes punctiferalis</i> Challenged with <i>Beauveria bassiana</i>
The yellow peach moth (YPM), <i>Conogethes punctiferalis</i>, is an important agricultural insect pest causing severe damage to corn in eastern China. <i>Beauveria bassiana</i> is an effective, eco-friendly, and promising alternative agent for controlling this insect pest. Ho...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/16/7/667 |
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| Summary: | The yellow peach moth (YPM), <i>Conogethes punctiferalis</i>, is an important agricultural insect pest causing severe damage to corn in eastern China. <i>Beauveria bassiana</i> is an effective, eco-friendly, and promising alternative agent for controlling this insect pest. However, insect immunity can limit the ability of fungal infections. In order to understand the immune response mechanism of YPM, a comparative proteomic analysis of non-infected and <i>B. bassiana</i>-infected larvae was conducted using the isobaric tags for relative and absolute quantification (iTRAQ) technique. On the basis of proteomic analysis, 4195 quantifiable proteins were identified from a total of 29,155 peptides. The functions of the identified proteins were annotated in four databases (GO, COG, KEGG, and IPR). A total of 132 immune-related proteins were screened, including 46 pathogen recognition proteins, 27 extracellular signal modulation proteins, and 59 immune effectors. Furthermore, 70 differentially expressed proteins (DEPs) were identified, including 57 up-regulated proteins and 13 down-regulated proteins. Among these, four DEPs were related to immunity, namely one defense protein and three peptidoglycan recognition proteins. Six randomly selected immune-related proteins associated with target genes were validated for qRT-PCR, and the results indicated that the accuracy and reliability of the proteome sequencing data were high. Taken together, the results enrich the fundamental knowledge of YPM immune responses to <i>B. bassiana</i> infection and provide a new insight into insect−pathogen interactions. |
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| ISSN: | 2075-4450 |