Enhancing Ruminant Fermentation with Bioethanol-Waste-Derived Yeast: In Vitro Evaluation of Digestibility, Gas Production, and NH? Concentration

This study analyzed the effect of yeast extracted from bioethanol waste as a direct-fed microorganism for ruminant livestock. Testing was conducted in vitro to assess the effect of yeast on ruminant livestock through dry matter and organic matter digestibility, gas production, and NH3 concentration....

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Main Authors: Khoiri Nurzaky, Marjuki, Dicky Pamungkas
Format: Article
Language:Indonesian
Published: Universitas Brawijaya 2025-03-01
Series:Jurnal Nutrisi Ternak Tropis
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Online Access:https://jnt.ub.ac.id/index.php/jnt/article/view/223
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author Khoiri Nurzaky
Marjuki
Dicky Pamungkas
author_facet Khoiri Nurzaky
Marjuki
Dicky Pamungkas
author_sort Khoiri Nurzaky
collection DOAJ
description This study analyzed the effect of yeast extracted from bioethanol waste as a direct-fed microorganism for ruminant livestock. Testing was conducted in vitro to assess the effect of yeast on ruminant livestock through dry matter and organic matter digestibility, gas production, and NH3 concentration. This study was divided into two phases. The first phase of research involved yeast cell cultivation and yeast powder production. The second research phase is an in vitro digestibility, gas production, and NH3 concentration analysis. The results of the first research phase were intended to obtain yeast samples that were cultivated using inoculum from purified bioethanol waste, which would be used for testing materials in the second research phase. The results of cell viability in processed yeast were 37.79%. The results of the second research phase showed that adding yeast as a feed additive to complete feed significantly increased the value of dry matter digestibility, organic matter digestibility, gas production, and NH3 concentration. The results of the in vitro study showed the highest dry matter digestibility (DMD) and organic matter digestibility (OMD) in the T3, which were 66.09% and 66.99%; the gas production results also showed the highest value in the T3 for total gas production 48h (161.15 ml/500 mg DM), gas production potential (171.24 ml/500 mg DM), and gas production rate (0.057 ml/hour). The T3 treatment also showed the highest NH3 concentration of 10.80 mM. The overall results of the research phases showed good results related to the use of yeast as an additive in complete feed, and the addition of 2% yeast gave the most significant results on the in vitro digestibility, gas production, and NH3 concentration.
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spelling doaj-art-e934b2c09e6a4f2b95d18ec04bbe9d022025-07-08T02:00:25ZindUniversitas BrawijayaJurnal Nutrisi Ternak Tropis2714-80682654-993X2025-03-0181516010.21776/ub.jnt.2025.008.01.6344Enhancing Ruminant Fermentation with Bioethanol-Waste-Derived Yeast: In Vitro Evaluation of Digestibility, Gas Production, and NH? ConcentrationKhoiri Nurzaky0Marjuki1Dicky Pamungkas2Faculty of Animal Science, Universitas Brawijaya, Malang, Jl. Veteran Malang, Ketawanggede, Malang, East Java 65145, IndonesiaFaculty of Animal Science, Universitas Brawijaya, Malang, Jl. Veteran Malang, Ketawanggede, Malang, East Java 65145, IndonesiaResearch Center for Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency of The Republic of Indonesia, Bogor 16911, IndonesiaThis study analyzed the effect of yeast extracted from bioethanol waste as a direct-fed microorganism for ruminant livestock. Testing was conducted in vitro to assess the effect of yeast on ruminant livestock through dry matter and organic matter digestibility, gas production, and NH3 concentration. This study was divided into two phases. The first phase of research involved yeast cell cultivation and yeast powder production. The second research phase is an in vitro digestibility, gas production, and NH3 concentration analysis. The results of the first research phase were intended to obtain yeast samples that were cultivated using inoculum from purified bioethanol waste, which would be used for testing materials in the second research phase. The results of cell viability in processed yeast were 37.79%. The results of the second research phase showed that adding yeast as a feed additive to complete feed significantly increased the value of dry matter digestibility, organic matter digestibility, gas production, and NH3 concentration. The results of the in vitro study showed the highest dry matter digestibility (DMD) and organic matter digestibility (OMD) in the T3, which were 66.09% and 66.99%; the gas production results also showed the highest value in the T3 for total gas production 48h (161.15 ml/500 mg DM), gas production potential (171.24 ml/500 mg DM), and gas production rate (0.057 ml/hour). The T3 treatment also showed the highest NH3 concentration of 10.80 mM. The overall results of the research phases showed good results related to the use of yeast as an additive in complete feed, and the addition of 2% yeast gave the most significant results on the in vitro digestibility, gas production, and NH3 concentration.https://jnt.ub.ac.id/index.php/jnt/article/view/223digestibilityfermentationgas productionin vitroyeast
spellingShingle Khoiri Nurzaky
Marjuki
Dicky Pamungkas
Enhancing Ruminant Fermentation with Bioethanol-Waste-Derived Yeast: In Vitro Evaluation of Digestibility, Gas Production, and NH? Concentration
Jurnal Nutrisi Ternak Tropis
digestibility
fermentation
gas production
in vitro
yeast
title Enhancing Ruminant Fermentation with Bioethanol-Waste-Derived Yeast: In Vitro Evaluation of Digestibility, Gas Production, and NH? Concentration
title_full Enhancing Ruminant Fermentation with Bioethanol-Waste-Derived Yeast: In Vitro Evaluation of Digestibility, Gas Production, and NH? Concentration
title_fullStr Enhancing Ruminant Fermentation with Bioethanol-Waste-Derived Yeast: In Vitro Evaluation of Digestibility, Gas Production, and NH? Concentration
title_full_unstemmed Enhancing Ruminant Fermentation with Bioethanol-Waste-Derived Yeast: In Vitro Evaluation of Digestibility, Gas Production, and NH? Concentration
title_short Enhancing Ruminant Fermentation with Bioethanol-Waste-Derived Yeast: In Vitro Evaluation of Digestibility, Gas Production, and NH? Concentration
title_sort enhancing ruminant fermentation with bioethanol waste derived yeast in vitro evaluation of digestibility gas production and nh concentration
topic digestibility
fermentation
gas production
in vitro
yeast
url https://jnt.ub.ac.id/index.php/jnt/article/view/223
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AT marjuki enhancingruminantfermentationwithbioethanolwastederivedyeastinvitroevaluationofdigestibilitygasproductionandnhconcentration
AT dickypamungkas enhancingruminantfermentationwithbioethanolwastederivedyeastinvitroevaluationofdigestibilitygasproductionandnhconcentration