Multi-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5′- and 3′ ends

Multi-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5′- and 3′ ends

Summary: CDK7 regulates RNA polymerase II (RNAPII) initiation, elongation, and termination through incompletely understood mechanisms. Because contaminating kinases prevent reliable CDK7 analysis with nuclear extracts, we reconstitute RNAPII transcription with purified factors. We show that CDK7 inh...

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Bibliographic Details
Main Authors: Olivia Luyties, Lynn Sanford, Jessica Rodino, Michael Nagel, Taylor Jones, Jenna K. Rimel, Christopher C. Ebmeier, Megan Palacio, Grace S. Shelby, Kira Cozzolino, Finn Brennan, Axel Hartzog, Mirzam B. Saucedo, Lotte P. Watts, Sabrina Spencer, Jennifer F. Kugel, Robin D. Dowell, Dylan J. Taatjes
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Cell Reports
Subjects:
CP: Molecular biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124725006758
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