Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels
Milk urea (MU) concentration is proposed as an indicator trait for breeding toward reduced nitrogen (N) emissions and leaching in dairy. We selected 20 German Holstein cows based on MU breeding values, with 10 cows each having low (LMUg) and high (HMUg) MU genetic predisposition. Using RNA-seq, we c...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2024.2322542 |
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| author | Henry Reyer Hanne Honerlagen Michael Oster Siriluck Ponsuksili Björn Kuhla Klaus Wimmers |
| author_facet | Henry Reyer Hanne Honerlagen Michael Oster Siriluck Ponsuksili Björn Kuhla Klaus Wimmers |
| author_sort | Henry Reyer |
| collection | DOAJ |
| description | Milk urea (MU) concentration is proposed as an indicator trait for breeding toward reduced nitrogen (N) emissions and leaching in dairy. We selected 20 German Holstein cows based on MU breeding values, with 10 cows each having low (LMUg) and high (HMUg) MU genetic predisposition. Using RNA-seq, we characterized these cows to unravel molecular pathways governing post-absorptive body N pools focusing on renal filtration and reabsorption of nitrogenous compounds, hepatic urea formation and mammary gland N excretion. While we observed minor adjustments in cellular energy metabolism in different tissues associated with different MU levels, no transcriptional differences in liver ammonia detoxification were detected, despite significant differences in MU between the groups. Differential expression of AQP3 and SLC38A2 in the kidney provides evidence for higher urea concentration in the collecting duct of LMU cows than HMU cows. The mammary gland exhibited the most significant differences, particularly in tricarboxylic acid (TCA) cycle genes, amino acid transport, tRNA binding, and casein synthesis. These findings suggest that selecting for lower MU could lead to altered urinary urea (UU) handling and changes in milk protein synthesis. However, given the genetic variability in N metabolism components, the long-term effectiveness of MU-based selection in reducing N emissions remains uncertain. |
| format | Article |
| id | doaj-art-e8787cadd7da45f59173f9dcaa9ca7d3 |
| institution | Matheson Library |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-e8787cadd7da45f59173f9dcaa9ca7d32025-07-23T08:23:43ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2024.2322542Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levelsHenry Reyer0Hanne Honerlagen1Michael Oster2Siriluck Ponsuksili3Björn Kuhla4Klaus Wimmers5Institute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Nutritional Physiology ‘Oskar Kellner’, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyMilk urea (MU) concentration is proposed as an indicator trait for breeding toward reduced nitrogen (N) emissions and leaching in dairy. We selected 20 German Holstein cows based on MU breeding values, with 10 cows each having low (LMUg) and high (HMUg) MU genetic predisposition. Using RNA-seq, we characterized these cows to unravel molecular pathways governing post-absorptive body N pools focusing on renal filtration and reabsorption of nitrogenous compounds, hepatic urea formation and mammary gland N excretion. While we observed minor adjustments in cellular energy metabolism in different tissues associated with different MU levels, no transcriptional differences in liver ammonia detoxification were detected, despite significant differences in MU between the groups. Differential expression of AQP3 and SLC38A2 in the kidney provides evidence for higher urea concentration in the collecting duct of LMU cows than HMU cows. The mammary gland exhibited the most significant differences, particularly in tricarboxylic acid (TCA) cycle genes, amino acid transport, tRNA binding, and casein synthesis. These findings suggest that selecting for lower MU could lead to altered urinary urea (UU) handling and changes in milk protein synthesis. However, given the genetic variability in N metabolism components, the long-term effectiveness of MU-based selection in reducing N emissions remains uncertain.https://www.tandfonline.com/doi/10.1080/10495398.2024.2322542RNAseqnitrogen emissionsmammary glandliverkidney |
| spellingShingle | Henry Reyer Hanne Honerlagen Michael Oster Siriluck Ponsuksili Björn Kuhla Klaus Wimmers Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels Animal Biotechnology RNAseq nitrogen emissions mammary gland liver kidney |
| title | Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels |
| title_full | Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels |
| title_fullStr | Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels |
| title_full_unstemmed | Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels |
| title_short | Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels |
| title_sort | multi tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels |
| topic | RNAseq nitrogen emissions mammary gland liver kidney |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2024.2322542 |
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