Genome-Wide Identification and Characterization of Trehalose-6-Phosphate Synthase/Phosphatases Gene Family in Petunia and Their Expression Profiling Under Abiotic Stresses
Trehalose is a nonreducing disaccharide critical for cellular integrity and stress adaptation in plants, and its synthesis relies on trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). Despite their established roles in abiotic stress responses across model plants, thes...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/11/6/695 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Trehalose is a nonreducing disaccharide critical for cellular integrity and stress adaptation in plants, and its synthesis relies on trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). Despite their established roles in abiotic stress responses across model plants, these gene families remain underexplored in ornamental species like <i>Petunia hybrida</i>. Here, <i>TPS</i> and <i>TPP</i> genes in two wild petunia progenitors, <i>P. axillaris</i> and <i>P. inflata</i>, underwent a genome-wide analysis, with 10 <i>TPS</i> and 8–9 <i>TPP</i> genes being identified in each species. According to phylogenetic analysis, petunia TPS proteins cluster into two clades, while TPP proteins were classified into three clades, showing closer evolutionary ties to tomato homologs. <i>Cis</i>-acting elements profiling identified hormone- and stress-responsive regulatory elements (e.g., ABRE, TC-rich repeats). Expression analysis under drought, heat, and salt stress revealed dynamic temporal regulation. For instance, <i>PaTPS4</i>/<i>PaTPS9</i> were early responders (peak at 6 h) under drought and salt stress, while <i>PaTPS8</i> exhibited sustained upregulation during salt treatment. Heat stress uniquely suppressed <i>PaTPS1</i>,<i>2</i> and <i>PaTPP1</i>, contrasting with broad upregulation of other members. Notably, <i>PaTPP3</i> displayed delayed induction under heat. These findings underscore the functional diversity within <i>TPS</i>/<i>TPP</i> families, with specific members governing stress-specific responses. This study provides a foundational resource for leveraging these genes to enhance stress resilience and ornamental value in petunia. |
|---|---|
| ISSN: | 2311-7524 |