FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearum
Fusarium graminearum is an important plant pathogenic fungus that causes disease and yield reduction in many cereal crops, such as wheat and barley. Gyp8 stimulates GTP hydrolysis on Ypt1 in yeast. However, the functions of Gyp8 in plant pathogenic fungi are still unknown. In this study, we investig...
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KeAi Communications Co., Ltd.
2023-11-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923000758 |
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| author | Xing-zhi ZHANG Shuang CHEN Yakubu Saddeeq ABUBAKAR Xu-zhao MAO Peng-fei MIAO Zong-hua WANG Jie ZHOU Hua-wei ZHENG |
| author_facet | Xing-zhi ZHANG Shuang CHEN Yakubu Saddeeq ABUBAKAR Xu-zhao MAO Peng-fei MIAO Zong-hua WANG Jie ZHOU Hua-wei ZHENG |
| author_sort | Xing-zhi ZHANG |
| collection | DOAJ |
| description | Fusarium graminearum is an important plant pathogenic fungus that causes disease and yield reduction in many cereal crops, such as wheat and barley. Gyp8 stimulates GTP hydrolysis on Ypt1 in yeast. However, the functions of Gyp8 in plant pathogenic fungi are still unknown. In this study, we investigated the roles of FgGyp8 in F. graminearum by genetic and pathological analyses. Through gene knockout and phenotypic analyses, we found that FgGyp8 is required for vegetative growth in F. graminearum. The conidiation, conidial size and number of septa per conidium of ΔFggyp8 mutant are significantly reduced when compared to the wild type PH-1. Furthermore, FgGyp8 is crucial for pathogenicity on wheat coleoptiles and wheat heads. FgGyp8 contains a conserved TBC domain. Domain deletion analysis showed that the TBC domain, C- and N-terminal regions of FgGyp8 are all important for its biological functions in F. graminearum. Moreover, we showed that FgGyp8 catalyzes the hydrolysis of the GTP on FgRab1 to GDP in vitro, indicating that FgGyp8 is a GTPase-activating protein (GAP) for FgRab1. In addition, we demonstrated that FgGyp8 is required for FgSnc1-mediated fusion of secretory vesicles with the plasma membrane in F. graminearum. Finally, we showed that FgGyp8 has functional redundancy with another FgRab1 GAP, FgGyp1, in F. graminearum. Taken together, we conclude that FgGyp8 is required for vegetative growth, conidiogenesis, pathogenicity and acts as a GAP for FgRab1 in F. graminearum. |
| format | Article |
| id | doaj-art-e7b42e2d060a4e7d9bb85502bd2c89a8 |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e7b42e2d060a4e7d9bb85502bd2c89a82025-08-02T22:13:20ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192023-11-01221134443457FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearumXing-zhi ZHANG0Shuang CHEN1Yakubu Saddeeq ABUBAKAR2Xu-zhao MAO3Peng-fei MIAO4Zong-hua WANG5Jie ZHOU6Hua-wei ZHENG7Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity/Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Minjiang University, Fuzhou 350108, P.R.China; Fujian University Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaFujian University Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaFujian University Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China; Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria 810211, NigeriaFujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity/Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Minjiang University, Fuzhou 350108, P.R.China; Fujian University Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaFujian University Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaFujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity/Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Minjiang University, Fuzhou 350108, P.R.China; Fujian University Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaFujian University Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China; Correspondence ZHOU JieFujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity/Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Minjiang University, Fuzhou 350108, P.R.China; Correspondence ZHENG Hua-weiFusarium graminearum is an important plant pathogenic fungus that causes disease and yield reduction in many cereal crops, such as wheat and barley. Gyp8 stimulates GTP hydrolysis on Ypt1 in yeast. However, the functions of Gyp8 in plant pathogenic fungi are still unknown. In this study, we investigated the roles of FgGyp8 in F. graminearum by genetic and pathological analyses. Through gene knockout and phenotypic analyses, we found that FgGyp8 is required for vegetative growth in F. graminearum. The conidiation, conidial size and number of septa per conidium of ΔFggyp8 mutant are significantly reduced when compared to the wild type PH-1. Furthermore, FgGyp8 is crucial for pathogenicity on wheat coleoptiles and wheat heads. FgGyp8 contains a conserved TBC domain. Domain deletion analysis showed that the TBC domain, C- and N-terminal regions of FgGyp8 are all important for its biological functions in F. graminearum. Moreover, we showed that FgGyp8 catalyzes the hydrolysis of the GTP on FgRab1 to GDP in vitro, indicating that FgGyp8 is a GTPase-activating protein (GAP) for FgRab1. In addition, we demonstrated that FgGyp8 is required for FgSnc1-mediated fusion of secretory vesicles with the plasma membrane in F. graminearum. Finally, we showed that FgGyp8 has functional redundancy with another FgRab1 GAP, FgGyp1, in F. graminearum. Taken together, we conclude that FgGyp8 is required for vegetative growth, conidiogenesis, pathogenicity and acts as a GAP for FgRab1 in F. graminearum.http://www.sciencedirect.com/science/article/pii/S2095311923000758Fusarium graminearumFgGyp8GTPase-activating proteinFgRab1conidiogenesispathogenicity |
| spellingShingle | Xing-zhi ZHANG Shuang CHEN Yakubu Saddeeq ABUBAKAR Xu-zhao MAO Peng-fei MIAO Zong-hua WANG Jie ZHOU Hua-wei ZHENG FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearum Journal of Integrative Agriculture Fusarium graminearum FgGyp8 GTPase-activating protein FgRab1 conidiogenesis pathogenicity |
| title | FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearum |
| title_full | FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearum |
| title_fullStr | FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearum |
| title_full_unstemmed | FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearum |
| title_short | FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearum |
| title_sort | fggyp8 as a putative fgrab1 gap is required for growth and pathogenesis by regulating fgsnc1 mediated secretory vesicles fusion in fusarium graminearum |
| topic | Fusarium graminearum FgGyp8 GTPase-activating protein FgRab1 conidiogenesis pathogenicity |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923000758 |
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