Transcriptomics and Metabolomics Reveal the Dwarfing Mechanism of Pepper Plants Under Ultraviolet Radiation
As a globally significant economic crop, pepper (<i>Capsicum annuum</i> L.) plants display excessive plant height (etiolation) in greenhouse production under an undesirable environment, leading to lodging-prone plants with reduced stress resistance. In the present study, we provided supp...
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MDPI AG
2025-07-01
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| author | Zejin Zhang Zhengnan Yan Xiangyu Ding Haoxu Shen Qi Liu Jinxiu Song Ying Liang Na Lu Li Tang |
| author_facet | Zejin Zhang Zhengnan Yan Xiangyu Ding Haoxu Shen Qi Liu Jinxiu Song Ying Liang Na Lu Li Tang |
| author_sort | Zejin Zhang |
| collection | DOAJ |
| description | As a globally significant economic crop, pepper (<i>Capsicum annuum</i> L.) plants display excessive plant height (etiolation) in greenhouse production under an undesirable environment, leading to lodging-prone plants with reduced stress resistance. In the present study, we provided supplementary ultraviolet-B (UV-B, 280–315 nm) light to pepper plants grown in a greenhouse to assess the influences of UV-B on pepper growth, with an emphasis on the molecular mechanisms mediated through the gibberellin (GA) signaling pathway. The results indicated that UV-B significantly decreased the plant height and the fresh weight of pepper plants. However, no significant differences were observed in the chlorophyll content of pepper plants grown under natural light and supplementary UV-B radiation. The results of the transcriptomic and metabolomic analyses indicated that differentially expressed genes (DEGs) were significantly enriched in plant hormone signal transduction and that UV radiation altered the gibberellin synthesis pathway of pepper plants. Specifically, the GA3 content of the pepper plants grown with UV-B radiation decreased by 39.1% compared with those grown without supplementary UV-B radiation; however, the opposite trend was observed in GA34, GA7, and GA51 contents. In conclusion, UV-B exposure significantly reduced plant height, a phenotypic response mechanistically linked to an alteration in GA homeostasis, which may be caused by a decrease in GA3 content. Our study elucidated the interplay between UV-B and gibberellin biosynthesis in pepper morphogenesis, offering a theoretical rationale for developing UV-B photoregulation technologies as alternatives to chemical growth inhibitors. |
| format | Article |
| id | doaj-art-e76d1e4e20d446dfa89469855a57e76d |
| institution | Matheson Library |
| issn | 2077-0472 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj-art-e76d1e4e20d446dfa89469855a57e76d2025-07-25T13:09:25ZengMDPI AGAgriculture2077-04722025-07-011514153510.3390/agriculture15141535Transcriptomics and Metabolomics Reveal the Dwarfing Mechanism of Pepper Plants Under Ultraviolet RadiationZejin Zhang0Zhengnan Yan1Xiangyu Ding2Haoxu Shen3Qi Liu4Jinxiu Song5Ying Liang6Na Lu7Li Tang8Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Horticulture, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, ChinaHorticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaCenter for Environment, Health and Field Sciences, Chiba University, 6-2-1 Kashiwanoha, Kashiwa 277-0882, Chiba, JapanHorticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaAs a globally significant economic crop, pepper (<i>Capsicum annuum</i> L.) plants display excessive plant height (etiolation) in greenhouse production under an undesirable environment, leading to lodging-prone plants with reduced stress resistance. In the present study, we provided supplementary ultraviolet-B (UV-B, 280–315 nm) light to pepper plants grown in a greenhouse to assess the influences of UV-B on pepper growth, with an emphasis on the molecular mechanisms mediated through the gibberellin (GA) signaling pathway. The results indicated that UV-B significantly decreased the plant height and the fresh weight of pepper plants. However, no significant differences were observed in the chlorophyll content of pepper plants grown under natural light and supplementary UV-B radiation. The results of the transcriptomic and metabolomic analyses indicated that differentially expressed genes (DEGs) were significantly enriched in plant hormone signal transduction and that UV radiation altered the gibberellin synthesis pathway of pepper plants. Specifically, the GA3 content of the pepper plants grown with UV-B radiation decreased by 39.1% compared with those grown without supplementary UV-B radiation; however, the opposite trend was observed in GA34, GA7, and GA51 contents. In conclusion, UV-B exposure significantly reduced plant height, a phenotypic response mechanistically linked to an alteration in GA homeostasis, which may be caused by a decrease in GA3 content. Our study elucidated the interplay between UV-B and gibberellin biosynthesis in pepper morphogenesis, offering a theoretical rationale for developing UV-B photoregulation technologies as alternatives to chemical growth inhibitors.https://www.mdpi.com/2077-0472/15/14/1535plant heightultraviolet radiationmetabolic pathwaypeppergibberellin |
| spellingShingle | Zejin Zhang Zhengnan Yan Xiangyu Ding Haoxu Shen Qi Liu Jinxiu Song Ying Liang Na Lu Li Tang Transcriptomics and Metabolomics Reveal the Dwarfing Mechanism of Pepper Plants Under Ultraviolet Radiation Agriculture plant height ultraviolet radiation metabolic pathway pepper gibberellin |
| title | Transcriptomics and Metabolomics Reveal the Dwarfing Mechanism of Pepper Plants Under Ultraviolet Radiation |
| title_full | Transcriptomics and Metabolomics Reveal the Dwarfing Mechanism of Pepper Plants Under Ultraviolet Radiation |
| title_fullStr | Transcriptomics and Metabolomics Reveal the Dwarfing Mechanism of Pepper Plants Under Ultraviolet Radiation |
| title_full_unstemmed | Transcriptomics and Metabolomics Reveal the Dwarfing Mechanism of Pepper Plants Under Ultraviolet Radiation |
| title_short | Transcriptomics and Metabolomics Reveal the Dwarfing Mechanism of Pepper Plants Under Ultraviolet Radiation |
| title_sort | transcriptomics and metabolomics reveal the dwarfing mechanism of pepper plants under ultraviolet radiation |
| topic | plant height ultraviolet radiation metabolic pathway pepper gibberellin |
| url | https://www.mdpi.com/2077-0472/15/14/1535 |
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