SNP-based identification of QTLs for thousand-grain weight and related traits in wheat 8762/Keyi 5214 DH lines
As important yield-related traits, thousand-grain weight (TGW), grain number per spike (GNS) and grain weight per spike (GWS) are crucial components of wheat production. To dissect their underlying genetic basis, a double haploid (DH) population comprised of 198 lines derived from 8762/Keyi 5214 was...
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KeAi Communications Co., Ltd.
2023-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923000576 |
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| author | Feng HUANG Xuan-shuang LI Xiao-yu DU Shun-cheng LI Nan-nan LI Yong-jun LÜ Shao-kui ZOU Qian ZHANG Li-na WANG Zhong-fu NI Yu-lin HAN Jie-wen XING |
| author_facet | Feng HUANG Xuan-shuang LI Xiao-yu DU Shun-cheng LI Nan-nan LI Yong-jun LÜ Shao-kui ZOU Qian ZHANG Li-na WANG Zhong-fu NI Yu-lin HAN Jie-wen XING |
| author_sort | Feng HUANG |
| collection | DOAJ |
| description | As important yield-related traits, thousand-grain weight (TGW), grain number per spike (GNS) and grain weight per spike (GWS) are crucial components of wheat production. To dissect their underlying genetic basis, a double haploid (DH) population comprised of 198 lines derived from 8762/Keyi 5214 was constructed. We then used genechip to genotype the DH population and integrated the yield-related traits TGW, GNS and GWS for QTL mapping. Finally, we obtained a total of 18 942 polymorphic SNP markers and identified 41 crucial QTLs for these traits. Three stable QTLs for TGW were identified on chromosomes 2D (QTgw-2D.3 and QTgw-2D.4) and 6A (QTgw-6A.1), with additive alleles all from the parent 8762, explaining 4.81–18.67% of the phenotypic variations. Five stable QTLs for GNS on chromosomes 3D, 5B, 5D and 6A were identified. QGns-5D.1 was from parent 8762, while the other four QTLs were from parent Keyi 5214, explaining 5.89–7.08% of the GNS phenotypic variations. In addition, a stable GWS genetic locus QGws-4A.3 was detected from the parent 8762, which explained 6.08–6.14% of the phenotypic variations. To utilize the identified QTLs, we developed STARP markers for four important QTLs, Tgw2D.3-2, Tgw2D.4-1, Tgw6A.1 and Gns3D.1. Our results provide important basic resources and references for the identification and cloning of genes related to TGW, GNS and GWS in wheat. |
| format | Article |
| id | doaj-art-e683fbb0746b4e3a92f9f19d74e9957a |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2023-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e683fbb0746b4e3a92f9f19d74e9957a2025-08-02T22:06:23ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192023-10-01221029492960SNP-based identification of QTLs for thousand-grain weight and related traits in wheat 8762/Keyi 5214 DH linesFeng HUANG0Xuan-shuang LI1Xiao-yu DU2Shun-cheng LI3Nan-nan LI4Yong-jun LÜ5Shao-kui ZOU6Qian ZHANG7Li-na WANG8Zhong-fu NI9Yu-lin HAN10Jie-wen XING11Zhoukou Academy of Agricultural Sciences, Zhoukou 466001, P.R.China; Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.ChinaZhoukou Academy of Agricultural Sciences, Zhoukou 466001, P.R.ChinaZhoukou Academy of Agricultural Sciences, Zhoukou 466001, P.R.ChinaZhoukou Academy of Agricultural Sciences, Zhoukou 466001, P.R.ChinaZhoukou Academy of Agricultural Sciences, Zhoukou 466001, P.R.ChinaZhoukou Academy of Agricultural Sciences, Zhoukou 466001, P.R.ChinaZhoukou Academy of Agricultural Sciences, Zhoukou 466001, P.R.ChinaZhoukou Academy of Agricultural Sciences, Zhoukou 466001, P.R.ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.ChinaZhoukou Academy of Agricultural Sciences, Zhoukou 466001, P.R.ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China; Correspondence XING Jie-wenAs important yield-related traits, thousand-grain weight (TGW), grain number per spike (GNS) and grain weight per spike (GWS) are crucial components of wheat production. To dissect their underlying genetic basis, a double haploid (DH) population comprised of 198 lines derived from 8762/Keyi 5214 was constructed. We then used genechip to genotype the DH population and integrated the yield-related traits TGW, GNS and GWS for QTL mapping. Finally, we obtained a total of 18 942 polymorphic SNP markers and identified 41 crucial QTLs for these traits. Three stable QTLs for TGW were identified on chromosomes 2D (QTgw-2D.3 and QTgw-2D.4) and 6A (QTgw-6A.1), with additive alleles all from the parent 8762, explaining 4.81–18.67% of the phenotypic variations. Five stable QTLs for GNS on chromosomes 3D, 5B, 5D and 6A were identified. QGns-5D.1 was from parent 8762, while the other four QTLs were from parent Keyi 5214, explaining 5.89–7.08% of the GNS phenotypic variations. In addition, a stable GWS genetic locus QGws-4A.3 was detected from the parent 8762, which explained 6.08–6.14% of the phenotypic variations. To utilize the identified QTLs, we developed STARP markers for four important QTLs, Tgw2D.3-2, Tgw2D.4-1, Tgw6A.1 and Gns3D.1. Our results provide important basic resources and references for the identification and cloning of genes related to TGW, GNS and GWS in wheat.http://www.sciencedirect.com/science/article/pii/S2095311923000576TGWGNSGWSQTL mappingwheat |
| spellingShingle | Feng HUANG Xuan-shuang LI Xiao-yu DU Shun-cheng LI Nan-nan LI Yong-jun LÜ Shao-kui ZOU Qian ZHANG Li-na WANG Zhong-fu NI Yu-lin HAN Jie-wen XING SNP-based identification of QTLs for thousand-grain weight and related traits in wheat 8762/Keyi 5214 DH lines Journal of Integrative Agriculture TGW GNS GWS QTL mapping wheat |
| title | SNP-based identification of QTLs for thousand-grain weight and related traits in wheat 8762/Keyi 5214 DH lines |
| title_full | SNP-based identification of QTLs for thousand-grain weight and related traits in wheat 8762/Keyi 5214 DH lines |
| title_fullStr | SNP-based identification of QTLs for thousand-grain weight and related traits in wheat 8762/Keyi 5214 DH lines |
| title_full_unstemmed | SNP-based identification of QTLs for thousand-grain weight and related traits in wheat 8762/Keyi 5214 DH lines |
| title_short | SNP-based identification of QTLs for thousand-grain weight and related traits in wheat 8762/Keyi 5214 DH lines |
| title_sort | snp based identification of qtls for thousand grain weight and related traits in wheat 8762 keyi 5214 dh lines |
| topic | TGW GNS GWS QTL mapping wheat |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923000576 |
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