miR-27b-5p regulates chicken liver disease via targeting IRS2 to suppress the PI3K/AKT signal pathway
The liver is a vital organ in chickens that performs a number of crucial physiological functions, including the storage of hepatic glycogen, protein synthesis, detoxification, and deoxidation. The growth and metabolism of the liver are complex processes influenced by factors such as environment, die...
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KeAi Communications Co., Ltd.
2023-11-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923000801 |
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| author | Jing ZHAO Ya-mei WU Yao ZHANG Shu-yue TANG Shun-shun HAN Can CUI Bo TAN Jie YU Hou-yang KANG Guang-deng CHEN Meng-gen MA Qing ZHU Hua-dong YIN |
| author_facet | Jing ZHAO Ya-mei WU Yao ZHANG Shu-yue TANG Shun-shun HAN Can CUI Bo TAN Jie YU Hou-yang KANG Guang-deng CHEN Meng-gen MA Qing ZHU Hua-dong YIN |
| author_sort | Jing ZHAO |
| collection | DOAJ |
| description | The liver is a vital organ in chickens that performs a number of crucial physiological functions, including the storage of hepatic glycogen, protein synthesis, detoxification, and deoxidation. The growth and metabolism of the liver are complex processes influenced by factors such as environment, diet, and genetics. MicroRNAs (miRNAs), as post-transcriptional regulatory molecules, play a role in various biological processes. There is growing evidence that miR-27b-5p plays a key role in the regulation of liver development and metabolism in various species. However, its role in chicken livers has yet to be determined. In our experiment, we found that chickens with fatty livers had significantly higher levels of serum triglyceride (TG) and total cholesterol (TC) compared to the normal chickens, while the control group had significantly higher levels of very low-density lipoprotein (VLDL) and serum hormones. Further research showed that the mRNA of miR-27b-5p was highly expressed in fatty livers. By exploring the function of miR-27b-5p in chicken livers, we discovered that it promotes lipogenesis, oxidative stress, and inflammatory responses, leading to hepatocyte apoptosis. Our study also established the mechanism by which miR-27b-5p interacts with its target gene, and found that miR-27b-5p targets insulin receptor substrate 2 (IRS2) and modulates the PI3K/AKT signaling pathway. Additionally, our investigation of IRS2 in chicken hepatocytes revealed that knocking down IRS2 has the same effects as overexpressing miR-27b-5p. In conclusion, our study revealed that miR-27b-5p directly binds to IRS2, inhibiting the PI3K/AKT signaling pathway and causing steatosis, oxidative stress, inflammation, and apoptosis in chicken liver. |
| format | Article |
| id | doaj-art-e660bfa97dfb45f68b4beb2d871a942d |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e660bfa97dfb45f68b4beb2d871a942d2025-08-02T22:05:43ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192023-11-01221135003516miR-27b-5p regulates chicken liver disease via targeting IRS2 to suppress the PI3K/AKT signal pathwayJing ZHAO0Ya-mei WU1Yao ZHANG2Shu-yue TANG3Shun-shun HAN4Can CUI5Bo TAN6Jie YU7Hou-yang KANG8Guang-deng CHEN9Meng-gen MA10Qing ZHU11Hua-dong YIN12Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaKey Laboratory for Animal Disease Resistance Nutrition of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, P.R.ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.China; Correspondence ZHU QingFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.China; Correspondence YIN Hua-dongThe liver is a vital organ in chickens that performs a number of crucial physiological functions, including the storage of hepatic glycogen, protein synthesis, detoxification, and deoxidation. The growth and metabolism of the liver are complex processes influenced by factors such as environment, diet, and genetics. MicroRNAs (miRNAs), as post-transcriptional regulatory molecules, play a role in various biological processes. There is growing evidence that miR-27b-5p plays a key role in the regulation of liver development and metabolism in various species. However, its role in chicken livers has yet to be determined. In our experiment, we found that chickens with fatty livers had significantly higher levels of serum triglyceride (TG) and total cholesterol (TC) compared to the normal chickens, while the control group had significantly higher levels of very low-density lipoprotein (VLDL) and serum hormones. Further research showed that the mRNA of miR-27b-5p was highly expressed in fatty livers. By exploring the function of miR-27b-5p in chicken livers, we discovered that it promotes lipogenesis, oxidative stress, and inflammatory responses, leading to hepatocyte apoptosis. Our study also established the mechanism by which miR-27b-5p interacts with its target gene, and found that miR-27b-5p targets insulin receptor substrate 2 (IRS2) and modulates the PI3K/AKT signaling pathway. Additionally, our investigation of IRS2 in chicken hepatocytes revealed that knocking down IRS2 has the same effects as overexpressing miR-27b-5p. In conclusion, our study revealed that miR-27b-5p directly binds to IRS2, inhibiting the PI3K/AKT signaling pathway and causing steatosis, oxidative stress, inflammation, and apoptosis in chicken liver.http://www.sciencedirect.com/science/article/pii/S2095311923000801miR-27b-5pIRS2lipogenesisoxidative stressinflammationapoptosis |
| spellingShingle | Jing ZHAO Ya-mei WU Yao ZHANG Shu-yue TANG Shun-shun HAN Can CUI Bo TAN Jie YU Hou-yang KANG Guang-deng CHEN Meng-gen MA Qing ZHU Hua-dong YIN miR-27b-5p regulates chicken liver disease via targeting IRS2 to suppress the PI3K/AKT signal pathway Journal of Integrative Agriculture miR-27b-5p IRS2 lipogenesis oxidative stress inflammation apoptosis |
| title | miR-27b-5p regulates chicken liver disease via targeting IRS2 to suppress the PI3K/AKT signal pathway |
| title_full | miR-27b-5p regulates chicken liver disease via targeting IRS2 to suppress the PI3K/AKT signal pathway |
| title_fullStr | miR-27b-5p regulates chicken liver disease via targeting IRS2 to suppress the PI3K/AKT signal pathway |
| title_full_unstemmed | miR-27b-5p regulates chicken liver disease via targeting IRS2 to suppress the PI3K/AKT signal pathway |
| title_short | miR-27b-5p regulates chicken liver disease via targeting IRS2 to suppress the PI3K/AKT signal pathway |
| title_sort | mir 27b 5p regulates chicken liver disease via targeting irs2 to suppress the pi3k akt signal pathway |
| topic | miR-27b-5p IRS2 lipogenesis oxidative stress inflammation apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923000801 |
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