Molecular Cloning, Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis)
The exocyst is a conserved protein complex, and required for vesicles tethering, fusion and polarized exocytosis. Exo70A1, the exocyst subunit, is essential for assembly of the exocyst complex. To better understand potential roles of Exo70A1 in non-heading Chinese cabbage (Brassica campestris ssp. c...
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KeAi Communications Co., Ltd.
2013-12-01
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| Loạt: | Journal of Integrative Agriculture |
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| Truy cập trực tuyến: | http://www.sciencedirect.com/science/article/pii/S2095311913605808 |
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| _version_ | 1839596099640229888 |
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| author | Li WANG Ting-ting GE Hai-tao PENG Cheng WANG Tong-kun LIU Xi-lin HOU Ying LI |
| author_facet | Li WANG Ting-ting GE Hai-tao PENG Cheng WANG Tong-kun LIU Xi-lin HOU Ying LI |
| author_sort | Li WANG |
| collection | DOAJ |
| description | The exocyst is a conserved protein complex, and required for vesicles tethering, fusion and polarized exocytosis. Exo70A1, the exocyst subunit, is essential for assembly of the exocyst complex. To better understand potential roles of Exo70A1 in non-heading Chinese cabbage (Brassica campestris ssp. chinensis), we obtained the full-length cDNA of Exo70A1 gene, which consisted of 1917 bp and encoded a protein of 638 amino acids. BlastX showed BcExo70A1 shared 94.9% identity with Brassica oleracea var. acephala (AEI26267.1), and clustered into a same group with other homologues in B. oleracea var. acephala and Brassica napus. Subcellular localization analysis showed BcExo70A1 was localized to punctate structures in cytosol of onion epithelial cells. Results showed that BcExo70A1 was widely presented in stamens, young stems, petals, unpollinated pistils, roots and leaves of self compatible and incompatible plants. The transcripts of BcExo70A1 in non-heading Chinese cabbage declined during initial 1.5 h after incompatible pollination, while an opposite trend was presented after compatible pollination. Our study reveals that BcExo70A1 could play essential roles in plant growth and development, and is related to the rejection of self pollen in non-heading Chinese cabbage. |
| format | Article |
| id | doaj-art-e63a4f4a358d4a0a9c61ba263d62c23a |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2013-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e63a4f4a358d4a0a9c61ba263d62c23a2025-08-02T22:04:21ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192013-12-01121221492156Molecular Cloning, Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis)Li WANG0Ting-ting GE1Hai-tao PENG2Cheng WANG3Tong-kun LIU4Xi-lin HOU5Ying LI6Horticultural Department, Nanjing Agricultural University, Nanjing 210095, P.R. China; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R. China; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, P.R. China; WANG Li, Tel: +86-25-84395756, Fax: +86-25-84395266Horticultural Department, Nanjing Agricultural University, Nanjing 210095, P.R. China; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R. China; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, P.R. ChinaHorticultural Department, Nanjing Agricultural University, Nanjing 210095, P.R. China; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R. China; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, P.R. ChinaHorticultural Department, Nanjing Agricultural University, Nanjing 210095, P.R. China; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R. China; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, P.R. ChinaHorticultural Department, Nanjing Agricultural University, Nanjing 210095, P.R. China; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R. China; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, P.R. ChinaHorticultural Department, Nanjing Agricultural University, Nanjing 210095, P.R. China; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R. China; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, P.R. ChinaHorticultural Department, Nanjing Agricultural University, Nanjing 210095, P.R. China; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R. China; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, P.R. China; Correspondence LI Ying, Tel: +86-25-84395756, Fax: +86-25-84395266The exocyst is a conserved protein complex, and required for vesicles tethering, fusion and polarized exocytosis. Exo70A1, the exocyst subunit, is essential for assembly of the exocyst complex. To better understand potential roles of Exo70A1 in non-heading Chinese cabbage (Brassica campestris ssp. chinensis), we obtained the full-length cDNA of Exo70A1 gene, which consisted of 1917 bp and encoded a protein of 638 amino acids. BlastX showed BcExo70A1 shared 94.9% identity with Brassica oleracea var. acephala (AEI26267.1), and clustered into a same group with other homologues in B. oleracea var. acephala and Brassica napus. Subcellular localization analysis showed BcExo70A1 was localized to punctate structures in cytosol of onion epithelial cells. Results showed that BcExo70A1 was widely presented in stamens, young stems, petals, unpollinated pistils, roots and leaves of self compatible and incompatible plants. The transcripts of BcExo70A1 in non-heading Chinese cabbage declined during initial 1.5 h after incompatible pollination, while an opposite trend was presented after compatible pollination. Our study reveals that BcExo70A1 could play essential roles in plant growth and development, and is related to the rejection of self pollen in non-heading Chinese cabbage.http://www.sciencedirect.com/science/article/pii/S2095311913605808Exo70A1gene expressionsubcellular localizationself-incompatibilitynon-heading Chinese cabbage |
| spellingShingle | Li WANG Ting-ting GE Hai-tao PENG Cheng WANG Tong-kun LIU Xi-lin HOU Ying LI Molecular Cloning, Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis) Journal of Integrative Agriculture Exo70A1 gene expression subcellular localization self-incompatibility non-heading Chinese cabbage |
| title | Molecular Cloning, Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis) |
| title_full | Molecular Cloning, Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis) |
| title_fullStr | Molecular Cloning, Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis) |
| title_full_unstemmed | Molecular Cloning, Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis) |
| title_short | Molecular Cloning, Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis) |
| title_sort | molecular cloning expression analysis and localization of exo70a1 related to self incompatibility in non heading chinese cabbage brassica campestris ssp chinensis |
| topic | Exo70A1 gene expression subcellular localization self-incompatibility non-heading Chinese cabbage |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913605808 |
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