INHIBITION OF CELLULAR INVASION AND INDUCTION OF ANOIKIS IN MOUSE MELANOMA CELLS BY AN ANTI-INFLAMMATORY AGENT DTCM-GLUTARIMIDE
Previously, we designed DTCM-glutarimide as a novel anti-inflammatory agent inhibiting macrophage activation. It also showed anti-inflammatory activity in vivo. In the present research, we evaluated the anti-metastatic activity of this agent using mouse melanoma B16-F10 cells. DTCM-glutarimide in...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bashkir State Medical University
2017-01-01
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| Series: | Креативная хирургия и онкология |
| Subjects: | |
| Online Access: | https://www.surgonco.ru/jour/article/view/123 |
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| Summary: | Previously, we designed DTCM-glutarimide as a novel anti-inflammatory agent inhibiting macrophage activation. It also showed anti-inflammatory activity in vivo. In the present research, we evaluated the anti-metastatic activity of this agent using mouse melanoma B16-F10 cells. DTCM-glutarimide inhibited the cellular invasion in the Matrigel chamber assay, lowering matrix metalloproteinase 9 (MMP9) expression. It also induced anoikis rather than apoptosis in polyHEMA-coated plates. It increased Bax and decreased Bcl-XL expressions, and activated p53 lowereing the MDM2 expression. Thus, DTCM-glutarimide was found to inhibit cellular invasion and to induce anoikis in B16-F10 cells. It is non-toxic, and easily prepared, therefore, is considered to be a new candidate of anti-metastasis agent. |
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| ISSN: | 2076-3093 2307-0501 |