Phenotypic Profiling of Biofilm Development and Efflux Pump Mechanisms in Multidrug-Resistant (MDR) Klebsiella pneumoniae
Study’s Excerpt: • All pneumoniae isolates were multidrug-resistant. • High resistance seen to Ofloxacin (75%) and Cephalexin (56%). • 25% of isolates formed biofilms; 12.5% showed efflux pump activity. • Ethidium bromide and Congo red agar were used for phenotypic tests. • Biofilms an...
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| Format: | Article |
| Language: | English |
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Umaru Musa Yar'adua University, Katsina, Nigeria
2025-06-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1150 |
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| author | Hadiza Na`'iya Misbahu Abdulhamid Yusuf Zahra Na`'iya Misbahu Dikwa, K. B. Shafa'atu Abubakar Bilkisu Isma'il Fadila Isyaku Adeleye Adewale Isaac |
| author_facet | Hadiza Na`'iya Misbahu Abdulhamid Yusuf Zahra Na`'iya Misbahu Dikwa, K. B. Shafa'atu Abubakar Bilkisu Isma'il Fadila Isyaku Adeleye Adewale Isaac |
| author_sort | Hadiza Na`'iya Misbahu |
| collection | DOAJ |
| description |
Study’s Excerpt:
• All pneumoniae isolates were multidrug-resistant.
• High resistance seen to Ofloxacin (75%) and Cephalexin (56%).
• 25% of isolates formed biofilms; 12.5% showed efflux pump activity.
• Ethidium bromide and Congo red agar were used for phenotypic tests.
• Biofilms and efflux pumps contribute to antibiotic resistance.
Full Abstract:
Klebsiella pneumoniae causes diverse healthcare- and community-associated infections, frequently affecting patients with indwelling medical devices. Its ability to form biofilms and activate efflux pumps enhances antibiotic resistance. These two mechanisms work synergistically, accelerating the emergence of multidrug-resistant K. pneumoniae strains..This study investigated the antibiotic resistance patterns of Klebsiella pneumoniae isolates in relation to their potential for biofilm formation and efflux pump activity. A total of Sixteen (16) K. pneumoniae isolates were obtained from the Department of Microbiology, University of Lagos, Lagos State. Confirmation of K. pneumoniae was conducted through culture, microscopy, and biochemical assays. Antibiotic susceptibility testing was performed using six routinely prescribed antibiotics. Phenotypic assessment of efflux pump activity and biofilm formation was conducted using the ethidium bromide (EtBr) cartwheel assay and Congo red agar method, respectively. The results indicated that all isolates were multidrug-resistant, exhibiting high resistance to Ofloxacin (12, 75.00%), Cephalexin (9, 56.25%), and Ampicillin (8, 50.00%). Four 4(25%)isolates demonstrated biofilm-forming ability, while only two 2(12.5%) isolates exhibited efflux pump activity. The results highlight biofilm formation and efflux pump activity as key virulence mechanisms contributing to the pathogenicity and multidrug resistance of K. pneumoniae, highlighting the need for targeted therapeutic strategies to overcome these resistance mechanisms.
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| format | Article |
| id | doaj-art-e5a207e91a6b43b4af061e7237a17d4d |
| institution | Matheson Library |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-e5a207e91a6b43b4af061e7237a17d4d2025-07-27T14:03:17ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222025-06-0110310.47430/ujmr.25103.015Phenotypic Profiling of Biofilm Development and Efflux Pump Mechanisms in Multidrug-Resistant (MDR) Klebsiella pneumoniaeHadiza Na`'iya Misbahu0Abdulhamid Yusuf1Zahra Na`'iya Misbahu2Dikwa, K. B.3Shafa'atu Abubakar4Bilkisu Isma'il5Fadila Isyaku6Adeleye Adewale Isaac7Department of Biological Sciences, Nigerian Defence Academy, Kaduna, NigeriaDepartment of Plant Science and Biotechnology, Federal University Dutsinma, Katsina, NigeriaDepartment of Microbiology, Umaru Musa Yar’adua University, Katsina, NigeriaDepartment of Biological Sciences, Nigerian Defence Academy, Kaduna, NigeriaDepartment of Biology, Usmanu Danfodiyo University, Sokoto, NigeriaDepartment of Biological Sciences, Nigerian Defence Academy, Kaduna, NigeriaDepartment of Biological Sciences, Nigerian Defence Academy, Kaduna, NigeriaDepartment of Microbiology, University of Lagos, Nigeria Study’s Excerpt: • All pneumoniae isolates were multidrug-resistant. • High resistance seen to Ofloxacin (75%) and Cephalexin (56%). • 25% of isolates formed biofilms; 12.5% showed efflux pump activity. • Ethidium bromide and Congo red agar were used for phenotypic tests. • Biofilms and efflux pumps contribute to antibiotic resistance. Full Abstract: Klebsiella pneumoniae causes diverse healthcare- and community-associated infections, frequently affecting patients with indwelling medical devices. Its ability to form biofilms and activate efflux pumps enhances antibiotic resistance. These two mechanisms work synergistically, accelerating the emergence of multidrug-resistant K. pneumoniae strains..This study investigated the antibiotic resistance patterns of Klebsiella pneumoniae isolates in relation to their potential for biofilm formation and efflux pump activity. A total of Sixteen (16) K. pneumoniae isolates were obtained from the Department of Microbiology, University of Lagos, Lagos State. Confirmation of K. pneumoniae was conducted through culture, microscopy, and biochemical assays. Antibiotic susceptibility testing was performed using six routinely prescribed antibiotics. Phenotypic assessment of efflux pump activity and biofilm formation was conducted using the ethidium bromide (EtBr) cartwheel assay and Congo red agar method, respectively. The results indicated that all isolates were multidrug-resistant, exhibiting high resistance to Ofloxacin (12, 75.00%), Cephalexin (9, 56.25%), and Ampicillin (8, 50.00%). Four 4(25%)isolates demonstrated biofilm-forming ability, while only two 2(12.5%) isolates exhibited efflux pump activity. The results highlight biofilm formation and efflux pump activity as key virulence mechanisms contributing to the pathogenicity and multidrug resistance of K. pneumoniae, highlighting the need for targeted therapeutic strategies to overcome these resistance mechanisms. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1150BiofilmEfflux pumpMultidrug-resistant (MDR)Klebsiella pneumoniaeAntibiotics |
| spellingShingle | Hadiza Na`'iya Misbahu Abdulhamid Yusuf Zahra Na`'iya Misbahu Dikwa, K. B. Shafa'atu Abubakar Bilkisu Isma'il Fadila Isyaku Adeleye Adewale Isaac Phenotypic Profiling of Biofilm Development and Efflux Pump Mechanisms in Multidrug-Resistant (MDR) Klebsiella pneumoniae UMYU Journal of Microbiology Research Biofilm Efflux pump Multidrug-resistant (MDR) Klebsiella pneumoniae Antibiotics |
| title | Phenotypic Profiling of Biofilm Development and Efflux Pump Mechanisms in Multidrug-Resistant (MDR) Klebsiella pneumoniae |
| title_full | Phenotypic Profiling of Biofilm Development and Efflux Pump Mechanisms in Multidrug-Resistant (MDR) Klebsiella pneumoniae |
| title_fullStr | Phenotypic Profiling of Biofilm Development and Efflux Pump Mechanisms in Multidrug-Resistant (MDR) Klebsiella pneumoniae |
| title_full_unstemmed | Phenotypic Profiling of Biofilm Development and Efflux Pump Mechanisms in Multidrug-Resistant (MDR) Klebsiella pneumoniae |
| title_short | Phenotypic Profiling of Biofilm Development and Efflux Pump Mechanisms in Multidrug-Resistant (MDR) Klebsiella pneumoniae |
| title_sort | phenotypic profiling of biofilm development and efflux pump mechanisms in multidrug resistant mdr klebsiella pneumoniae |
| topic | Biofilm Efflux pump Multidrug-resistant (MDR) Klebsiella pneumoniae Antibiotics |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1150 |
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