Novel mouse models for tracing brain-derived extracellular vesicles
Extracellular vesicles (EVs), particularly small extracellular vesicles (sEVs) such as exosomes, are carriers of biomolecules that reflect the metabolic status of host cells. Recently, sEVs have emerged as potential reservoirs of molecular biomarkers associated with pathological conditions, includin...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-12-01
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| Series: | Extracellular Vesicle |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773041725000241 |
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| author | Qi Wu Fanxiu Ma Meng Yuan Xiumei Jin Zhaodi Jiang Jiyan Ma |
| author_facet | Qi Wu Fanxiu Ma Meng Yuan Xiumei Jin Zhaodi Jiang Jiyan Ma |
| author_sort | Qi Wu |
| collection | DOAJ |
| description | Extracellular vesicles (EVs), particularly small extracellular vesicles (sEVs) such as exosomes, are carriers of biomolecules that reflect the metabolic status of host cells. Recently, sEVs have emerged as potential reservoirs of molecular biomarkers associated with pathological conditions, including cancers, neurodegenerative disorders, and cardiovascular diseases. While blood-based tests are common diagnostic tools, their efficacy in identifying central nervous system (CNS) diseases is significantly hindered by the blood-brain barrier, which prevents the entry of brain-derived proteins into the bloodstream. Capturing brain-derived sEVs (BDEs) in peripheral blood is a potential alternative for detecting CNS disease biomarkers. However, the presence of BDEs in blood remains obscure due to the lack of specific labeling. To address this challenge, we established two novel mouse models in which neuron-derived sEVs or EVs from specific brain regions were fluorescently labeled. Using these approaches we successfully detected fluorescently labeled EVs in both BDEs and plasma-derived sEVs (PDEs), confirming that BDEs are indeed present in peripheral blood. These models will serve as valuable tools for developing novel methods to efficiently capture BDEs in peripheral blood. |
| format | Article |
| id | doaj-art-e58e6e3f3b23456f8c25a21048b8f9a2 |
| institution | Matheson Library |
| issn | 2773-0417 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Extracellular Vesicle |
| spelling | doaj-art-e58e6e3f3b23456f8c25a21048b8f9a22025-07-11T04:32:03ZengElsevierExtracellular Vesicle2773-04172025-12-016100087Novel mouse models for tracing brain-derived extracellular vesiclesQi Wu0Fanxiu Ma1Meng Yuan2Xiumei Jin3Zhaodi Jiang4Jiyan Ma5Beijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China; Chinese Institute for Brain Research, Beijing (CIBR), Beijing, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China; Chinese Institute for Brain Research, Beijing (CIBR), Beijing, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China; Chinese Institute for Brain Research, Beijing (CIBR), Beijing, ChinaNational Institute of Biological Sciences, Beijing, ChinaNational Institute of Biological Sciences, Beijing, China; Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China; Chinese Institute for Brain Research, Beijing (CIBR), Beijing, China; Corresponding author. Chinese Institute for Brain Research, Beijing (CIBR), A6 Bldg.3, NO.9, YIKE Rd, Zhongguancun Life Science Park, Changping District, Beijing, 102206, China.Extracellular vesicles (EVs), particularly small extracellular vesicles (sEVs) such as exosomes, are carriers of biomolecules that reflect the metabolic status of host cells. Recently, sEVs have emerged as potential reservoirs of molecular biomarkers associated with pathological conditions, including cancers, neurodegenerative disorders, and cardiovascular diseases. While blood-based tests are common diagnostic tools, their efficacy in identifying central nervous system (CNS) diseases is significantly hindered by the blood-brain barrier, which prevents the entry of brain-derived proteins into the bloodstream. Capturing brain-derived sEVs (BDEs) in peripheral blood is a potential alternative for detecting CNS disease biomarkers. However, the presence of BDEs in blood remains obscure due to the lack of specific labeling. To address this challenge, we established two novel mouse models in which neuron-derived sEVs or EVs from specific brain regions were fluorescently labeled. Using these approaches we successfully detected fluorescently labeled EVs in both BDEs and plasma-derived sEVs (PDEs), confirming that BDEs are indeed present in peripheral blood. These models will serve as valuable tools for developing novel methods to efficiently capture BDEs in peripheral blood.http://www.sciencedirect.com/science/article/pii/S2773041725000241Small extracellular vesiclesFlow cytometrySuper-resolution microscopyBrain derived EVs |
| spellingShingle | Qi Wu Fanxiu Ma Meng Yuan Xiumei Jin Zhaodi Jiang Jiyan Ma Novel mouse models for tracing brain-derived extracellular vesicles Extracellular Vesicle Small extracellular vesicles Flow cytometry Super-resolution microscopy Brain derived EVs |
| title | Novel mouse models for tracing brain-derived extracellular vesicles |
| title_full | Novel mouse models for tracing brain-derived extracellular vesicles |
| title_fullStr | Novel mouse models for tracing brain-derived extracellular vesicles |
| title_full_unstemmed | Novel mouse models for tracing brain-derived extracellular vesicles |
| title_short | Novel mouse models for tracing brain-derived extracellular vesicles |
| title_sort | novel mouse models for tracing brain derived extracellular vesicles |
| topic | Small extracellular vesicles Flow cytometry Super-resolution microscopy Brain derived EVs |
| url | http://www.sciencedirect.com/science/article/pii/S2773041725000241 |
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