In-House IgM Dot-Blot Assay for Serodiagnosis of Human Leptospirosis: Development, Standardisation, and Performance Evaluation

In-House IgM Dot-Blot Assay for Serodiagnosis of Human Leptospirosis: Development, Standardisation, and Performance Evaluation

Laboratory confirmation of human leptospirosis relies on serological tests, with the microscopic agglutination test (MAT) as the reference. However, due to its complexity, there is a need for a simpler and more accessible diagnostic method. This study aimed to standardise and develop an IgM dot-blot...

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Bibliographic Details
Main Authors: Roberta Morozetti Blanco, Elaine dos Santos Lima, Juliana Maira Watanabe Pinhata, Angela Pires Brandao, Eliete Caló Romero
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Microorganisms
Subjects:
leptospirosis
serodiagnosis
whole-cell antigen
dot-blot
Online Access:https://www.mdpi.com/2076-2607/13/6/1307
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