Cortical spreading depression produces a neuroprotective effect activating mitochondrial uncoupling protein-5
Emanuela Viggiano,1,2 Vincenzo Monda,1 Antonietta Messina,1 Fiorenzo Moscatelli,3 Anna Valenzano,3 Domenico Tafuri,4 Giuseppe Cibelli,3 Bruno De Luca,1 Giovanni Messina,1,3 Marcellino Monda1 1Department of Experimental Medicine, Section of Human Physiology and Unit of Dietetics and Sports Medicine,...
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Dove Medical Press
2016-07-01
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| Series: | Neuropsychiatric Disease and Treatment |
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| Online Access: | https://www.dovepress.com/cortical-spreading-depression-produces-a-neuroprotective-effect-activa-peer-reviewed-fulltext-article-NDT |
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| author | Viggiano E Monda V Messina A Moscatelli F Valenzano A Tafuri D Cibelli G De Luca B Messina G Monda M |
| author_facet | Viggiano E Monda V Messina A Moscatelli F Valenzano A Tafuri D Cibelli G De Luca B Messina G Monda M |
| author_sort | Viggiano E |
| collection | DOAJ |
| description | Emanuela Viggiano,1,2 Vincenzo Monda,1 Antonietta Messina,1 Fiorenzo Moscatelli,3 Anna Valenzano,3 Domenico Tafuri,4 Giuseppe Cibelli,3 Bruno De Luca,1 Giovanni Messina,1,3 Marcellino Monda1 1Department of Experimental Medicine, Section of Human Physiology and Unit of Dietetics and Sports Medicine, Second University of Naples, Naples, 2Department of Medicine, University of Padua, Padua, 3Department of Clinical and Experimental Medicine, University of Foggia, Foggia, 4Department of Motor Sciences and Wellness, University of Naples “Parthenope”, Naples, Italy Abstract: Depression of electrocorticogram propagating over the cortex surface results in cortical spreading depression (CSD), which is probably related to the pathophysiology of stroke, epilepsy, and migraine. However, preconditioning with CSD produces neuroprotection to subsequent ischemic episodes. Such effects require the expression or activation of several genes, including neuroprotective ones. Recently, it has been demonstrated that the expression of the uncoupling proteins (UCPs) 2 and 5 is amplified during brain ischemia and their expression exerts a long-term effect upon neuron protection. To evaluate the neuroprotective consequence of CSD, the expression of UCP-5 in the brain cortex was measured following CSD induction. CSD was evoked in four samples of rats, which were sacrificed after 2 hours, 4 hours, 6 hours, and 24 hours. Western blot analyses were carried out to measure UCP-5 concentrations in the prefrontal cortices of both hemispheres, and immunohistochemistry was performed to determine the localization of UCP-5 in the brain cortex. The results showed a significant elevation in UCP-5 expression at 24 hours in all cortical strata. Moreover, UCP-5 was triggered by CSD, indicating that UCP-5 production can have a neuroprotective effect. Keywords: cortical spreading depression, neuroprotective effect, uncoupling protein-5A Letter to the Editor has been received and published for this article. |
| format | Article |
| id | doaj-art-e51a2cbbfda542bd90b08d9f73bc9b13 |
| institution | Matheson Library |
| issn | 1178-2021 |
| language | English |
| publishDate | 2016-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Neuropsychiatric Disease and Treatment |
| spelling | doaj-art-e51a2cbbfda542bd90b08d9f73bc9b132025-06-26T01:58:36ZengDove Medical PressNeuropsychiatric Disease and Treatment1178-20212016-07-01Volume 12Issue 11705171027843Cortical spreading depression produces a neuroprotective effect activating mitochondrial uncoupling protein-5Viggiano EMonda VMessina AMoscatelli FValenzano ATafuri DCibelli GDe Luca BMessina G0Monda MDepartment of Experimental MedicineEmanuela Viggiano,1,2 Vincenzo Monda,1 Antonietta Messina,1 Fiorenzo Moscatelli,3 Anna Valenzano,3 Domenico Tafuri,4 Giuseppe Cibelli,3 Bruno De Luca,1 Giovanni Messina,1,3 Marcellino Monda1 1Department of Experimental Medicine, Section of Human Physiology and Unit of Dietetics and Sports Medicine, Second University of Naples, Naples, 2Department of Medicine, University of Padua, Padua, 3Department of Clinical and Experimental Medicine, University of Foggia, Foggia, 4Department of Motor Sciences and Wellness, University of Naples “Parthenope”, Naples, Italy Abstract: Depression of electrocorticogram propagating over the cortex surface results in cortical spreading depression (CSD), which is probably related to the pathophysiology of stroke, epilepsy, and migraine. However, preconditioning with CSD produces neuroprotection to subsequent ischemic episodes. Such effects require the expression or activation of several genes, including neuroprotective ones. Recently, it has been demonstrated that the expression of the uncoupling proteins (UCPs) 2 and 5 is amplified during brain ischemia and their expression exerts a long-term effect upon neuron protection. To evaluate the neuroprotective consequence of CSD, the expression of UCP-5 in the brain cortex was measured following CSD induction. CSD was evoked in four samples of rats, which were sacrificed after 2 hours, 4 hours, 6 hours, and 24 hours. Western blot analyses were carried out to measure UCP-5 concentrations in the prefrontal cortices of both hemispheres, and immunohistochemistry was performed to determine the localization of UCP-5 in the brain cortex. The results showed a significant elevation in UCP-5 expression at 24 hours in all cortical strata. Moreover, UCP-5 was triggered by CSD, indicating that UCP-5 production can have a neuroprotective effect. Keywords: cortical spreading depression, neuroprotective effect, uncoupling protein-5A Letter to the Editor has been received and published for this article.https://www.dovepress.com/cortical-spreading-depression-produces-a-neuroprotective-effect-activa-peer-reviewed-fulltext-article-NDTCortical Spreading Depressionneuroprotective effectUCP5 |
| spellingShingle | Viggiano E Monda V Messina A Moscatelli F Valenzano A Tafuri D Cibelli G De Luca B Messina G Monda M Cortical spreading depression produces a neuroprotective effect activating mitochondrial uncoupling protein-5 Neuropsychiatric Disease and Treatment Cortical Spreading Depression neuroprotective effect UCP5 |
| title | Cortical spreading depression produces a neuroprotective effect activating mitochondrial uncoupling protein-5 |
| title_full | Cortical spreading depression produces a neuroprotective effect activating mitochondrial uncoupling protein-5 |
| title_fullStr | Cortical spreading depression produces a neuroprotective effect activating mitochondrial uncoupling protein-5 |
| title_full_unstemmed | Cortical spreading depression produces a neuroprotective effect activating mitochondrial uncoupling protein-5 |
| title_short | Cortical spreading depression produces a neuroprotective effect activating mitochondrial uncoupling protein-5 |
| title_sort | cortical spreading depression produces a neuroprotective effect activating mitochondrial uncoupling protein 5 |
| topic | Cortical Spreading Depression neuroprotective effect UCP5 |
| url | https://www.dovepress.com/cortical-spreading-depression-produces-a-neuroprotective-effect-activa-peer-reviewed-fulltext-article-NDT |
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