Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance
Plant defensins are small, basic cysteine-rich peptides that play important roles in disease resistance. A gene, designated BoDFN, was isolated from Brassica oleracea var. italica. Gene sequence has been submitted to NCBI with an accession no. of HQ436486. Complete coding sequence of BoDFN is 243 bp...
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KeAi Communications Co., Ltd.
2012-07-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311912601075 |
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| author | Ming JIANG Cai-ming HE Li-xiang MIAO Yu-chao ZHANG |
| author_facet | Ming JIANG Cai-ming HE Li-xiang MIAO Yu-chao ZHANG |
| author_sort | Ming JIANG |
| collection | DOAJ |
| description | Plant defensins are small, basic cysteine-rich peptides that play important roles in disease resistance. A gene, designated BoDFN, was isolated from Brassica oleracea var. italica. Gene sequence has been submitted to NCBI with an accession no. of HQ436486. Complete coding sequence of BoDFN is 243 bp in length encoding 80 amino acids. Sequence comparison results showed that BoDFN shared high homology with those of crucifer plants and there were only few DNA base differences. RT-PCR results indicated an increase of gene expression in Hyaloperonospora parasitica infected leaves and revealed a significant increase at 24 and 36 h of treatment. A recombinant plasmid, named pBI121-BoDFN, was constructed and introduced into Agrobacterium tumefacien LBA4404. PCR screening for 65 regenerated plantlets, 17 positive plantlets were obtained, PCR screening results revealed that 17 out of 65 regenerated plantlets were positive. Disease resistant identification results indicated that all positive plants showed an increase in resistance to H. parasitica. |
| format | Article |
| id | doaj-art-e37ff7331d0a4a9f9d8056f48bee4a5a |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2012-07-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e37ff7331d0a4a9f9d8056f48bee4a5a2025-08-02T21:48:21ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192012-07-0111711371144Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew ResistanceMing JIANG0Cai-ming HE1Li-xiang MIAO2Yu-chao ZHANG3College of Life Science, Taizhou University, Linhai 317000, P.R. China; Correspondence JIANG Ming, Tel: +86-576-85305853, Mobile: 15057232729College of Life Science, Taizhou University, Linhai 317000, P.R. ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, P.R. ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, P.R. ChinaPlant defensins are small, basic cysteine-rich peptides that play important roles in disease resistance. A gene, designated BoDFN, was isolated from Brassica oleracea var. italica. Gene sequence has been submitted to NCBI with an accession no. of HQ436486. Complete coding sequence of BoDFN is 243 bp in length encoding 80 amino acids. Sequence comparison results showed that BoDFN shared high homology with those of crucifer plants and there were only few DNA base differences. RT-PCR results indicated an increase of gene expression in Hyaloperonospora parasitica infected leaves and revealed a significant increase at 24 and 36 h of treatment. A recombinant plasmid, named pBI121-BoDFN, was constructed and introduced into Agrobacterium tumefacien LBA4404. PCR screening for 65 regenerated plantlets, 17 positive plantlets were obtained, PCR screening results revealed that 17 out of 65 regenerated plantlets were positive. Disease resistant identification results indicated that all positive plants showed an increase in resistance to H. parasitica.http://www.sciencedirect.com/science/article/pii/S2095311912601075broccolidowny mildewdefensinoverexpression |
| spellingShingle | Ming JIANG Cai-ming HE Li-xiang MIAO Yu-chao ZHANG Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance Journal of Integrative Agriculture broccoli downy mildew defensin overexpression |
| title | Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance |
| title_full | Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance |
| title_fullStr | Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance |
| title_full_unstemmed | Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance |
| title_short | Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance |
| title_sort | overexpression of a broccoli defensin gene bodfn enhances downy mildew resistance |
| topic | broccoli downy mildew defensin overexpression |
| url | http://www.sciencedirect.com/science/article/pii/S2095311912601075 |
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