An SSR-Base Linkage Map Reveals QTLs for Floral-Related Traits in Nightlily (<i>Hemerocallis citrina</i>)
Nightlily (<i>Hemerocallis citrina</i> <b>Baroni</b>) is mainly cultivated for bud consumption with medicinal, nutritional, and economic value. Enhancing nightlily yield is one of the most significant breeding goals of modern agriculture of <i>H. citrina</i> breed...
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2025-06-01
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| author | Yuting Su Zhonghao Liang Xinyu Zhao Lijing Shi Yang Liu Yang Gao Xiaojing Cheng Guoming Xing Sen Li |
| author_facet | Yuting Su Zhonghao Liang Xinyu Zhao Lijing Shi Yang Liu Yang Gao Xiaojing Cheng Guoming Xing Sen Li |
| author_sort | Yuting Su |
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| description | Nightlily (<i>Hemerocallis citrina</i> <b>Baroni</b>) is mainly cultivated for bud consumption with medicinal, nutritional, and economic value. Enhancing nightlily yield is one of the most significant breeding goals of modern agriculture of <i>H. citrina</i> breeding objective, but it also faces great challenges. In this study, an intraspecific hybridization population crossed between two varieties, ‘Liuyuehua’ and ‘Datong Huanghua’ of Hemerocallis, was used to establish 715 F<sub>1</sub> individuals. Phenotypic data for eight floral traits, including scape number, bud number, scape length, scape diameter, bud length, bud diameter, fresh flower bud weight, and dry flower bud weight, were collected from 715 F<sub>1</sub> individuals over a three-year period (2022, 2023, and 2024). The Simple Repeat Sequence (SSR) markers were validated to genotype the 116 random F<sub>1</sub> individuals and to construct a linkage map. The intraspecific hybridization map contained 11 linkage groups. A total of 169 SSR markers were used to construct a linkage map, spanning a total map length of 1605.3 cM, with an average marker interval of 9.50 cM. The linkage map revealed 11 floral QTLs from 7.21% to 24.29% of phenotypic variation. Through collinearity analysis, it was found that 122 markers could be aligned to the published genome sequence of <i>H. citrina</i>. A total of five candidate genes for floral traits were predicted. The linkage map is essential for mapping and marker-assisted progeny selection that will accelerate the pace of nightlily breeding. |
| format | Article |
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| issn | 2073-4395 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj-art-e31dda3685e44ca4844bd987e3966e212025-07-25T13:09:54ZengMDPI AGAgronomy2073-43952025-06-01157159910.3390/agronomy15071599An SSR-Base Linkage Map Reveals QTLs for Floral-Related Traits in Nightlily (<i>Hemerocallis citrina</i>)Yuting Su0Zhonghao Liang1Xinyu Zhao2Lijing Shi3Yang Liu4Yang Gao5Xiaojing Cheng6Guoming Xing7Sen Li8College of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu, Jinzhong 030801, ChinaNightlily (<i>Hemerocallis citrina</i> <b>Baroni</b>) is mainly cultivated for bud consumption with medicinal, nutritional, and economic value. Enhancing nightlily yield is one of the most significant breeding goals of modern agriculture of <i>H. citrina</i> breeding objective, but it also faces great challenges. In this study, an intraspecific hybridization population crossed between two varieties, ‘Liuyuehua’ and ‘Datong Huanghua’ of Hemerocallis, was used to establish 715 F<sub>1</sub> individuals. Phenotypic data for eight floral traits, including scape number, bud number, scape length, scape diameter, bud length, bud diameter, fresh flower bud weight, and dry flower bud weight, were collected from 715 F<sub>1</sub> individuals over a three-year period (2022, 2023, and 2024). The Simple Repeat Sequence (SSR) markers were validated to genotype the 116 random F<sub>1</sub> individuals and to construct a linkage map. The intraspecific hybridization map contained 11 linkage groups. A total of 169 SSR markers were used to construct a linkage map, spanning a total map length of 1605.3 cM, with an average marker interval of 9.50 cM. The linkage map revealed 11 floral QTLs from 7.21% to 24.29% of phenotypic variation. Through collinearity analysis, it was found that 122 markers could be aligned to the published genome sequence of <i>H. citrina</i>. A total of five candidate genes for floral traits were predicted. The linkage map is essential for mapping and marker-assisted progeny selection that will accelerate the pace of nightlily breeding.https://www.mdpi.com/2073-4395/15/7/1599nightlilySSRyield traitslinkage mapQTL mapping |
| spellingShingle | Yuting Su Zhonghao Liang Xinyu Zhao Lijing Shi Yang Liu Yang Gao Xiaojing Cheng Guoming Xing Sen Li An SSR-Base Linkage Map Reveals QTLs for Floral-Related Traits in Nightlily (<i>Hemerocallis citrina</i>) Agronomy nightlily SSR yield traits linkage map QTL mapping |
| title | An SSR-Base Linkage Map Reveals QTLs for Floral-Related Traits in Nightlily (<i>Hemerocallis citrina</i>) |
| title_full | An SSR-Base Linkage Map Reveals QTLs for Floral-Related Traits in Nightlily (<i>Hemerocallis citrina</i>) |
| title_fullStr | An SSR-Base Linkage Map Reveals QTLs for Floral-Related Traits in Nightlily (<i>Hemerocallis citrina</i>) |
| title_full_unstemmed | An SSR-Base Linkage Map Reveals QTLs for Floral-Related Traits in Nightlily (<i>Hemerocallis citrina</i>) |
| title_short | An SSR-Base Linkage Map Reveals QTLs for Floral-Related Traits in Nightlily (<i>Hemerocallis citrina</i>) |
| title_sort | ssr base linkage map reveals qtls for floral related traits in nightlily i hemerocallis citrina i |
| topic | nightlily SSR yield traits linkage map QTL mapping |
| url | https://www.mdpi.com/2073-4395/15/7/1599 |
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