Clumpy Novel Mitochondrial Signatures in Irradiated Human Diabetic Buccal Cells: A Case Control Study
Objective: This study aimed to determine the whole free mitochondria in type-2 diabetic buccal epithelial cells using a supravital stain called 'Janus Green B' and assess their behavior after exposure to near infrared light. The researchers observed microscopic mitochondrial load and its i...
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Galenos Publishing House
2025-06-01
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| Series: | Medeniyet Medical Journal |
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| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=medeniyet&un=MEDJ-87120 |
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| author | Surraj SUSAI Mrudula CHANDRUPATLA Sumitra SIVAKOTI Govindrao N. KUSNENIWAR Anand K. PYATI |
| author_facet | Surraj SUSAI Mrudula CHANDRUPATLA Sumitra SIVAKOTI Govindrao N. KUSNENIWAR Anand K. PYATI |
| author_sort | Surraj SUSAI |
| collection | DOAJ |
| description | Objective: This study aimed to determine the whole free mitochondria in type-2 diabetic buccal epithelial cells using a supravital stain called 'Janus Green B' and assess their behavior after exposure to near infrared light. The researchers observed microscopic mitochondrial load and its intracellular spatial behavior after exposure to near-infrared rays, bridging the gap in understanding mitochondrial orientation in diseases like diabetes. The aim of this research was to find out the quantitative involvement and electrostatic intracellular spatial patterns of whole mitochondria in diabetes.
Methods: Exfoliated buccal cell wet mounts, supravitally stained using Janus green, and excited using infrared rays, were observed using an advanced bright field Axiocam microscope, and the images of whole mitochondria within the cells were photographed and analyzed using the ZEN 2.0 cell sense software. The migration patterns of mitochondria were observed.
Results: A quantitative decrease in mitochondria was noted in diabetic cells. Signatures of clumpy peripheral shifts in mitochondria were observed in diabetic buccal cells post radiation.
Conclusions: Advanced glycation end products of diabetes combined with oxidative stressors influenced the free mitochondria to clump peripherally and produce a characteristic signature. The decreased mitochondrial load contributed additional evidence to the reduced respiratory capacity of cells, which forced mitochondria to emit a detectable signature when irradiated. |
| format | Article |
| id | doaj-art-e1036ab9f8654167aae65cc34e135b8f |
| institution | Matheson Library |
| issn | 2149-2042 2149-4606 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Medeniyet Medical Journal |
| spelling | doaj-art-e1036ab9f8654167aae65cc34e135b8f2025-07-18T07:34:27ZengGalenos Publishing HouseMedeniyet Medical Journal2149-20422149-46062025-06-014029310010.4274/MMJ.galenos.2025.87120MEDJ-87120Clumpy Novel Mitochondrial Signatures in Irradiated Human Diabetic Buccal Cells: A Case Control StudySurraj SUSAI0Mrudula CHANDRUPATLA1Sumitra SIVAKOTI2Govindrao N. KUSNENIWAR3Anand K. PYATI4All India Institute of Medical Sciences, Department of Anatomy, Bibinagar, IndiaAll India Institute of Medical Sciences, Department of Anatomy, Bibinagar, IndiaAll India Institute of Medical Sciences, Department of Pathology, Bibinagar, IndiaAll India Institute of Medical Sciences, Department of Community medicine, Bibinagar, IndiaAll India Institute of Medical Sciences, Department of Biochemistry, Bibinagar, IndiaObjective: This study aimed to determine the whole free mitochondria in type-2 diabetic buccal epithelial cells using a supravital stain called 'Janus Green B' and assess their behavior after exposure to near infrared light. The researchers observed microscopic mitochondrial load and its intracellular spatial behavior after exposure to near-infrared rays, bridging the gap in understanding mitochondrial orientation in diseases like diabetes. The aim of this research was to find out the quantitative involvement and electrostatic intracellular spatial patterns of whole mitochondria in diabetes. Methods: Exfoliated buccal cell wet mounts, supravitally stained using Janus green, and excited using infrared rays, were observed using an advanced bright field Axiocam microscope, and the images of whole mitochondria within the cells were photographed and analyzed using the ZEN 2.0 cell sense software. The migration patterns of mitochondria were observed. Results: A quantitative decrease in mitochondria was noted in diabetic cells. Signatures of clumpy peripheral shifts in mitochondria were observed in diabetic buccal cells post radiation. Conclusions: Advanced glycation end products of diabetes combined with oxidative stressors influenced the free mitochondria to clump peripherally and produce a characteristic signature. The decreased mitochondrial load contributed additional evidence to the reduced respiratory capacity of cells, which forced mitochondria to emit a detectable signature when irradiated.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=medeniyet&un=MEDJ-87120peripheral clumpsinfra red rayspower housesintracellularshiftcomets |
| spellingShingle | Surraj SUSAI Mrudula CHANDRUPATLA Sumitra SIVAKOTI Govindrao N. KUSNENIWAR Anand K. PYATI Clumpy Novel Mitochondrial Signatures in Irradiated Human Diabetic Buccal Cells: A Case Control Study Medeniyet Medical Journal peripheral clumps infra red rays power houses intracellular shift comets |
| title | Clumpy Novel Mitochondrial Signatures in Irradiated Human Diabetic Buccal Cells: A Case Control Study |
| title_full | Clumpy Novel Mitochondrial Signatures in Irradiated Human Diabetic Buccal Cells: A Case Control Study |
| title_fullStr | Clumpy Novel Mitochondrial Signatures in Irradiated Human Diabetic Buccal Cells: A Case Control Study |
| title_full_unstemmed | Clumpy Novel Mitochondrial Signatures in Irradiated Human Diabetic Buccal Cells: A Case Control Study |
| title_short | Clumpy Novel Mitochondrial Signatures in Irradiated Human Diabetic Buccal Cells: A Case Control Study |
| title_sort | clumpy novel mitochondrial signatures in irradiated human diabetic buccal cells a case control study |
| topic | peripheral clumps infra red rays power houses intracellular shift comets |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=medeniyet&un=MEDJ-87120 |
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