The mechanism of miR-16-5p protection on LPS-induced A549 cell injury by targeting CXCR3
Objective To study the effect of miR-16-5p on lung cancer cell injury and apoptosis, and its mechanism.Methods LPS induced lung cancer cell A549 injury; qRT-PCR method was applied to detect the expression of miR-16-5p and CXCR3 in A549 cells. Con (without LPS treatment), LPS + miR-NC group (transfec...
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1593998 |
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| author | Guo-Pan Liu Wei-Wei Wang Wen-Ying Lu An-Quan Shang |
| author_facet | Guo-Pan Liu Wei-Wei Wang Wen-Ying Lu An-Quan Shang |
| author_sort | Guo-Pan Liu |
| collection | DOAJ |
| description | Objective To study the effect of miR-16-5p on lung cancer cell injury and apoptosis, and its mechanism.Methods LPS induced lung cancer cell A549 injury; qRT-PCR method was applied to detect the expression of miR-16-5p and CXCR3 in A549 cells. Con (without LPS treatment), LPS + miR-NC group (transfected negative control samples), LPS + miR-16-5p group (transfected miR-16-5p mimics); LPS + si-NC group (transfected negative control samples), LPS + si-CXCR3 group (transfected si-CXCR3); LPS + miR-16-5p + pcDNA3.1 group (co-transfected miR-16-5p mimics and pcDNA3.1), LPS + miR-16-5p + pcDNA3.1-CXCR3 group (co-transfected miR-16-5p mimics and pcDNA3.1-CXCR3) were transfected into A549 cells by liposome method. Western blot was used to detect protein expression of CXCR3, IL-6 and TNF-α in A549 cells; apoptosis of A549 cells was detected by flow cytometry.Results Compared with the control group, the expression of miR-16-5p mRNA was significantly decreased in A549 cells in LPS group, and the mRNA and protein expression of CXCR3 were significantly increased (p < .05). Overexpression of miR-16-5p and knockdown of CXCR3 both can down-regulated protein expression of IL-6 and TNF-α, and up-regulated apoptosis in LPS-induced A549 cell; CXCR3 is a target of miR-16-5p. Overexpression of CXCR3 rescued the protective effect of miR-16-5p on LPS-induced A549 cell injury.Conclusion miR-16-5p can protect LPS-induced A549 cell injury, and its mechanism may be related to the targeted regulation of CXCR3, which could provide a new target for targeted therapy of lung cancer. |
| format | Article |
| id | doaj-art-def4e5a23e9b47e890a34e22b4eb6dd8 |
| institution | Matheson Library |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
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| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-def4e5a23e9b47e890a34e22b4eb6dd82025-07-21T21:25:01ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014711200120610.1080/21691401.2019.1593998The mechanism of miR-16-5p protection on LPS-induced A549 cell injury by targeting CXCR3Guo-Pan Liu0Wei-Wei Wang1Wen-Ying Lu2An-Quan Shang3College of Clinical Medicine, Ningxia Medical University, Yinchuan, P.R. ChinaDepartment of Pathology, The Sixth People’s Hospital of Yancheng City, Yancheng, P.R. ChinaDepartment of Pathology, The Sixth People’s Hospital of Yancheng City, Yancheng, P.R. ChinaDepartment of Laboratory Medicine, Tongji Hospital of Tongji University, Shanghai, P.R. ChinaObjective To study the effect of miR-16-5p on lung cancer cell injury and apoptosis, and its mechanism.Methods LPS induced lung cancer cell A549 injury; qRT-PCR method was applied to detect the expression of miR-16-5p and CXCR3 in A549 cells. Con (without LPS treatment), LPS + miR-NC group (transfected negative control samples), LPS + miR-16-5p group (transfected miR-16-5p mimics); LPS + si-NC group (transfected negative control samples), LPS + si-CXCR3 group (transfected si-CXCR3); LPS + miR-16-5p + pcDNA3.1 group (co-transfected miR-16-5p mimics and pcDNA3.1), LPS + miR-16-5p + pcDNA3.1-CXCR3 group (co-transfected miR-16-5p mimics and pcDNA3.1-CXCR3) were transfected into A549 cells by liposome method. Western blot was used to detect protein expression of CXCR3, IL-6 and TNF-α in A549 cells; apoptosis of A549 cells was detected by flow cytometry.Results Compared with the control group, the expression of miR-16-5p mRNA was significantly decreased in A549 cells in LPS group, and the mRNA and protein expression of CXCR3 were significantly increased (p < .05). Overexpression of miR-16-5p and knockdown of CXCR3 both can down-regulated protein expression of IL-6 and TNF-α, and up-regulated apoptosis in LPS-induced A549 cell; CXCR3 is a target of miR-16-5p. Overexpression of CXCR3 rescued the protective effect of miR-16-5p on LPS-induced A549 cell injury.Conclusion miR-16-5p can protect LPS-induced A549 cell injury, and its mechanism may be related to the targeted regulation of CXCR3, which could provide a new target for targeted therapy of lung cancer.https://www.tandfonline.com/doi/10.1080/21691401.2019.1593998miR-16-5pCXCR3lung cancerapoptosis |
| spellingShingle | Guo-Pan Liu Wei-Wei Wang Wen-Ying Lu An-Quan Shang The mechanism of miR-16-5p protection on LPS-induced A549 cell injury by targeting CXCR3 Artificial Cells, Nanomedicine, and Biotechnology miR-16-5p CXCR3 lung cancer apoptosis |
| title | The mechanism of miR-16-5p protection on LPS-induced A549 cell injury by targeting CXCR3 |
| title_full | The mechanism of miR-16-5p protection on LPS-induced A549 cell injury by targeting CXCR3 |
| title_fullStr | The mechanism of miR-16-5p protection on LPS-induced A549 cell injury by targeting CXCR3 |
| title_full_unstemmed | The mechanism of miR-16-5p protection on LPS-induced A549 cell injury by targeting CXCR3 |
| title_short | The mechanism of miR-16-5p protection on LPS-induced A549 cell injury by targeting CXCR3 |
| title_sort | mechanism of mir 16 5p protection on lps induced a549 cell injury by targeting cxcr3 |
| topic | miR-16-5p CXCR3 lung cancer apoptosis |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1593998 |
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