In Vitro Evaluation of the Ovicidal Potential of Proteases from <i>Beauveria bassiana</i> Against <i>Eurytrema pancreaticum</i> Eggs
In the search for new alternatives for controlling parasitic agents, proteases from <i>Beauveria bassiana</i> stand out. The aim of this study was to evaluate in silico and in vitro the ovicidal potential of <i>B. bassiana</i> proteases on <i>Eurytrema pancreaticum</...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Microbiology Research |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2036-7481/16/6/127 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839653307592736768 |
|---|---|
| author | Lisseth Bibiana Puentes Figueroa Amanda do Carmo Alves Adriane Toledo da Silva Debora Castro de Souza Nivia Kelly Lima Sales Lorrana Verdi Flores Tiago Facury Moreira Fabio Ribeiro Braga Filippe Elias de Freitas Soares |
| author_facet | Lisseth Bibiana Puentes Figueroa Amanda do Carmo Alves Adriane Toledo da Silva Debora Castro de Souza Nivia Kelly Lima Sales Lorrana Verdi Flores Tiago Facury Moreira Fabio Ribeiro Braga Filippe Elias de Freitas Soares |
| author_sort | Lisseth Bibiana Puentes Figueroa |
| collection | DOAJ |
| description | In the search for new alternatives for controlling parasitic agents, proteases from <i>Beauveria bassiana</i> stand out. The aim of this study was to evaluate in silico and in vitro the ovicidal potential of <i>B. bassiana</i> proteases on <i>Eurytrema pancreaticum</i> Janson, 1889 (Dicrocoeliidae) eggs. <i>Beauveria bassiana</i> Bals. -Criv., 1835 (Cordycipitaceae) (IP 361) was cultivated for enzymatic production. Proteins were precipitated with acetone (1:4 ratio), and specific activity was determined. Protease profiles were assessed via zymography, and inhibition by phenylmethylsulfonyl fluoride (PMSF) and ethylenediaminetetraacetic acid (EDTA) was tested. Three-dimensional models of the proteases were generated. <i>Eurytrema pancreaticum</i> eggs were used for the in vitro anthelmintic evaluation of the proteases. The results showed that precipitation significantly concentrated proteolytic activity (<i>p</i> < 0.01) compared to the crude extract. However, no chitinase activity was detected. The proteolytic profile of the precipitate revealed five bands with molecular weights from 25.6 to 66.9 kDa. In the in vitro tests, the proteases significantly (<i>p</i> < 0.01) reduced the number of intact <i>E. pancreaticum</i> eggs by 53% compared to the control with denatured enzymes. These findings highlight the ovicidal potential of <i>B. bassiana</i> proteases, though further studies are needed to confirm their application in parasite control. |
| format | Article |
| id | doaj-art-ddbb815135a04cf39daccf00702937a1 |
| institution | Matheson Library |
| issn | 2036-7481 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj-art-ddbb815135a04cf39daccf00702937a12025-06-25T14:11:19ZengMDPI AGMicrobiology Research2036-74812025-06-0116612710.3390/microbiolres16060127In Vitro Evaluation of the Ovicidal Potential of Proteases from <i>Beauveria bassiana</i> Against <i>Eurytrema pancreaticum</i> EggsLisseth Bibiana Puentes Figueroa0Amanda do Carmo Alves1Adriane Toledo da Silva2Debora Castro de Souza3Nivia Kelly Lima Sales4Lorrana Verdi Flores5Tiago Facury Moreira6Fabio Ribeiro Braga7Filippe Elias de Freitas Soares8Laboratório de Biotecnologia e Bioquímica Aplicada, Departamento de Química, Universidade Federal de Lavras, Lavras 37200-000, MG, BrazilLaboratório de Biotecnologia e Bioquímica Aplicada, Departamento de Química, Universidade Federal de Lavras, Lavras 37200-000, MG, BrazilLaboratório de Biotecnologia e Bioquímica Aplicada, Departamento de Química, Universidade Federal de Lavras, Lavras 37200-000, MG, BrazilLaboratório de Biotecnologia e Bioquímica Aplicada, Departamento de Química, Universidade Federal de Lavras, Lavras 37200-000, MG, BrazilLaboratório de Biotecnologia e Bioquímica Aplicada, Departamento de Química, Universidade Federal de Lavras, Lavras 37200-000, MG, BrazilLaboratório Central de Biologia Molecular, Departamento de Química, Universidade Federal de Lavras, Lavras 37200-000, MG, BrazilEscola de Veterinária, Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-800, MG, BrazilLaboratório de Parasitologia Experimental e Controle Biológico, Universidade Vila-Velha, Vila Velha 29102-920, ES, BrazilLaboratório de Biotecnologia e Bioquímica Aplicada, Departamento de Química, Universidade Federal de Lavras, Lavras 37200-000, MG, BrazilIn the search for new alternatives for controlling parasitic agents, proteases from <i>Beauveria bassiana</i> stand out. The aim of this study was to evaluate in silico and in vitro the ovicidal potential of <i>B. bassiana</i> proteases on <i>Eurytrema pancreaticum</i> Janson, 1889 (Dicrocoeliidae) eggs. <i>Beauveria bassiana</i> Bals. -Criv., 1835 (Cordycipitaceae) (IP 361) was cultivated for enzymatic production. Proteins were precipitated with acetone (1:4 ratio), and specific activity was determined. Protease profiles were assessed via zymography, and inhibition by phenylmethylsulfonyl fluoride (PMSF) and ethylenediaminetetraacetic acid (EDTA) was tested. Three-dimensional models of the proteases were generated. <i>Eurytrema pancreaticum</i> eggs were used for the in vitro anthelmintic evaluation of the proteases. The results showed that precipitation significantly concentrated proteolytic activity (<i>p</i> < 0.01) compared to the crude extract. However, no chitinase activity was detected. The proteolytic profile of the precipitate revealed five bands with molecular weights from 25.6 to 66.9 kDa. In the in vitro tests, the proteases significantly (<i>p</i> < 0.01) reduced the number of intact <i>E. pancreaticum</i> eggs by 53% compared to the control with denatured enzymes. These findings highlight the ovicidal potential of <i>B. bassiana</i> proteases, though further studies are needed to confirm their application in parasite control.https://www.mdpi.com/2036-7481/16/6/127entomopathogenic fungusproteaseeurythrematosis3D structurezymogram |
| spellingShingle | Lisseth Bibiana Puentes Figueroa Amanda do Carmo Alves Adriane Toledo da Silva Debora Castro de Souza Nivia Kelly Lima Sales Lorrana Verdi Flores Tiago Facury Moreira Fabio Ribeiro Braga Filippe Elias de Freitas Soares In Vitro Evaluation of the Ovicidal Potential of Proteases from <i>Beauveria bassiana</i> Against <i>Eurytrema pancreaticum</i> Eggs Microbiology Research entomopathogenic fungus protease eurythrematosis 3D structure zymogram |
| title | In Vitro Evaluation of the Ovicidal Potential of Proteases from <i>Beauveria bassiana</i> Against <i>Eurytrema pancreaticum</i> Eggs |
| title_full | In Vitro Evaluation of the Ovicidal Potential of Proteases from <i>Beauveria bassiana</i> Against <i>Eurytrema pancreaticum</i> Eggs |
| title_fullStr | In Vitro Evaluation of the Ovicidal Potential of Proteases from <i>Beauveria bassiana</i> Against <i>Eurytrema pancreaticum</i> Eggs |
| title_full_unstemmed | In Vitro Evaluation of the Ovicidal Potential of Proteases from <i>Beauveria bassiana</i> Against <i>Eurytrema pancreaticum</i> Eggs |
| title_short | In Vitro Evaluation of the Ovicidal Potential of Proteases from <i>Beauveria bassiana</i> Against <i>Eurytrema pancreaticum</i> Eggs |
| title_sort | in vitro evaluation of the ovicidal potential of proteases from i beauveria bassiana i against i eurytrema pancreaticum i eggs |
| topic | entomopathogenic fungus protease eurythrematosis 3D structure zymogram |
| url | https://www.mdpi.com/2036-7481/16/6/127 |
| work_keys_str_mv | AT lissethbibianapuentesfigueroa invitroevaluationoftheovicidalpotentialofproteasesfromibeauveriabassianaiagainstieurytremapancreaticumieggs AT amandadocarmoalves invitroevaluationoftheovicidalpotentialofproteasesfromibeauveriabassianaiagainstieurytremapancreaticumieggs AT adrianetoledodasilva invitroevaluationoftheovicidalpotentialofproteasesfromibeauveriabassianaiagainstieurytremapancreaticumieggs AT deboracastrodesouza invitroevaluationoftheovicidalpotentialofproteasesfromibeauveriabassianaiagainstieurytremapancreaticumieggs AT niviakellylimasales invitroevaluationoftheovicidalpotentialofproteasesfromibeauveriabassianaiagainstieurytremapancreaticumieggs AT lorranaverdiflores invitroevaluationoftheovicidalpotentialofproteasesfromibeauveriabassianaiagainstieurytremapancreaticumieggs AT tiagofacurymoreira invitroevaluationoftheovicidalpotentialofproteasesfromibeauveriabassianaiagainstieurytremapancreaticumieggs AT fabioribeirobraga invitroevaluationoftheovicidalpotentialofproteasesfromibeauveriabassianaiagainstieurytremapancreaticumieggs AT filippeeliasdefreitassoares invitroevaluationoftheovicidalpotentialofproteasesfromibeauveriabassianaiagainstieurytremapancreaticumieggs |