Assessment of Animal Sensitivity to Particularly Dangerous Orthopoxviruses, Using Primary Cultures of Lung Cells
Objective of the study is to investigate the sensitivity of different animals to highly pathogenic Orthopoxviruses applying techniques, based on utilization of primary cultures of lung cells, and to assess the possibility of further deployment of this approach. Materials and methods. Cultural and vi...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2016-03-01
|
| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/290 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839636890370703360 |
|---|---|
| author | A. S. Zamedyanskaya Al. A. Sergeev K. A. Titova A. S. Kabanov L. E. Bulychev Ar. A. Sergeev A. E. Nesterov O. V. Nosareva D. O. Galakhova L. N. Shishkina A. P. Agafonov A. N. Sergeev |
| author_facet | A. S. Zamedyanskaya Al. A. Sergeev K. A. Titova A. S. Kabanov L. E. Bulychev Ar. A. Sergeev A. E. Nesterov O. V. Nosareva D. O. Galakhova L. N. Shishkina A. P. Agafonov A. N. Sergeev |
| author_sort | A. S. Zamedyanskaya |
| collection | DOAJ |
| description | Objective of the study is to investigate the sensitivity of different animals to highly pathogenic Orthopoxviruses applying techniques, based on utilization of primary cultures of lung cells, and to assess the possibility of further deployment of this approach. Materials and methods. Cultural and virological research methods are used. Results and conclusions. Performed is the assessment of sensitivity of outbred mice, marmots and chickens to variola virus (VV) and monkeypox virus (MPV), using suspended primary cultures of lung cells (SPCLC) of these animals. Through inoculation of the mentioned above cell cultures with VV and MPV in a dose of 0.00001 PFU per a cell (plaque forming unit /cell) demonstrated has been virus replication with maximum concentration values in all cases (1,4 - 2,0 lg PFU/ml), mainly 3 days after infection. According to the data on SPCLC, sensitivity to VV in mice, marmots and chickens (ID50 - 50 % infective dose) amounts to (1,3 ± 0,5) lg PFU; (2,3 ± 0,5) lg PFU; and (0,0 ± 0,4) lg PFU respectively, taking into account unhindered interaction of the virus with permissive lung cells in the organism of the animals. As for MPV values for this indicator, they are: (1,7 ± 0,3) lg PFU for mice, and (0,5 ± 0,3) lg PFU - for marmots. Obtained ID50 values for VV using mice SPCLC and for MPV using mice and marmots SPCLC coincide with the ones, studied in direct experiments on intranasal infection with the viruses, with regard to 10 % of the viral application in lungs when deploying the latter method of infection. The fact testifies to the possibility of further deployment of this method for the assessment of animal sensitivity to highly pathogenic Orthopoxviruses based on the results of in vitro experiments. |
| format | Article |
| id | doaj-art-dd9f597aece14c038df1adf618d2cad7 |
| institution | Matheson Library |
| issn | 0370-1069 2658-719X |
| language | Russian |
| publishDate | 2016-03-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj-art-dd9f597aece14c038df1adf618d2cad72025-07-07T08:02:39ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2016-03-0101757810.21055/0370-1069-2016-1-75-78290Assessment of Animal Sensitivity to Particularly Dangerous Orthopoxviruses, Using Primary Cultures of Lung CellsA. S. Zamedyanskaya0Al. A. Sergeev1K. A. Titova2A. S. Kabanov3L. E. Bulychev4Ar. A. Sergeev5A. E. Nesterov6O. V. Nosareva7D. O. Galakhova8L. N. Shishkina9A. P. Agafonov10A. N. Sergeev11State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”Objective of the study is to investigate the sensitivity of different animals to highly pathogenic Orthopoxviruses applying techniques, based on utilization of primary cultures of lung cells, and to assess the possibility of further deployment of this approach. Materials and methods. Cultural and virological research methods are used. Results and conclusions. Performed is the assessment of sensitivity of outbred mice, marmots and chickens to variola virus (VV) and monkeypox virus (MPV), using suspended primary cultures of lung cells (SPCLC) of these animals. Through inoculation of the mentioned above cell cultures with VV and MPV in a dose of 0.00001 PFU per a cell (plaque forming unit /cell) demonstrated has been virus replication with maximum concentration values in all cases (1,4 - 2,0 lg PFU/ml), mainly 3 days after infection. According to the data on SPCLC, sensitivity to VV in mice, marmots and chickens (ID50 - 50 % infective dose) amounts to (1,3 ± 0,5) lg PFU; (2,3 ± 0,5) lg PFU; and (0,0 ± 0,4) lg PFU respectively, taking into account unhindered interaction of the virus with permissive lung cells in the organism of the animals. As for MPV values for this indicator, they are: (1,7 ± 0,3) lg PFU for mice, and (0,5 ± 0,3) lg PFU - for marmots. Obtained ID50 values for VV using mice SPCLC and for MPV using mice and marmots SPCLC coincide with the ones, studied in direct experiments on intranasal infection with the viruses, with regard to 10 % of the viral application in lungs when deploying the latter method of infection. The fact testifies to the possibility of further deployment of this method for the assessment of animal sensitivity to highly pathogenic Orthopoxviruses based on the results of in vitro experiments.https://journal.microbe.ru/jour/article/view/290вирус натуральной оспывирус оспы обезьянмышьсурокцыпленокпервичные клетки легких50 % инфицирующая дозавирусная продукцияvariola virusmonkeypox virusmousemarmotchickenprimary lung cells50 % infective doseviral production |
| spellingShingle | A. S. Zamedyanskaya Al. A. Sergeev K. A. Titova A. S. Kabanov L. E. Bulychev Ar. A. Sergeev A. E. Nesterov O. V. Nosareva D. O. Galakhova L. N. Shishkina A. P. Agafonov A. N. Sergeev Assessment of Animal Sensitivity to Particularly Dangerous Orthopoxviruses, Using Primary Cultures of Lung Cells Проблемы особо опасных инфекций вирус натуральной оспы вирус оспы обезьян мышь сурок цыпленок первичные клетки легких 50 % инфицирующая доза вирусная продукция variola virus monkeypox virus mouse marmot chicken primary lung cells 50 % infective dose viral production |
| title | Assessment of Animal Sensitivity to Particularly Dangerous Orthopoxviruses, Using Primary Cultures of Lung Cells |
| title_full | Assessment of Animal Sensitivity to Particularly Dangerous Orthopoxviruses, Using Primary Cultures of Lung Cells |
| title_fullStr | Assessment of Animal Sensitivity to Particularly Dangerous Orthopoxviruses, Using Primary Cultures of Lung Cells |
| title_full_unstemmed | Assessment of Animal Sensitivity to Particularly Dangerous Orthopoxviruses, Using Primary Cultures of Lung Cells |
| title_short | Assessment of Animal Sensitivity to Particularly Dangerous Orthopoxviruses, Using Primary Cultures of Lung Cells |
| title_sort | assessment of animal sensitivity to particularly dangerous orthopoxviruses using primary cultures of lung cells |
| topic | вирус натуральной оспы вирус оспы обезьян мышь сурок цыпленок первичные клетки легких 50 % инфицирующая доза вирусная продукция variola virus monkeypox virus mouse marmot chicken primary lung cells 50 % infective dose viral production |
| url | https://journal.microbe.ru/jour/article/view/290 |
| work_keys_str_mv | AT aszamedyanskaya assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells AT alasergeev assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells AT katitova assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells AT askabanov assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells AT lebulychev assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells AT arasergeev assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells AT aenesterov assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells AT ovnosareva assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells AT dogalakhova assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells AT lnshishkina assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells AT apagafonov assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells AT ansergeev assessmentofanimalsensitivitytoparticularlydangerousorthopoxvirusesusingprimaryculturesoflungcells |