Hydrophobin-like proteins in aerial conidia of three entomopathogenic hyphmycetes as fungal biocontrol agents: contents and SDS-PAGE profiles
Contents of hydrophobin-like proteins (HLP) extracted from aerial conidia of 14 isolates of Beauveria bassiana, Paecilomyces fumosoroseus and Metarhizium anisopliae varied significantly among the three fungal species and their isolates. Of those, the maximal HLP content in B. bassiana 2861 conidia w...
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Zhejiang University Press
2006-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2006.04.0401 |
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| author | YING Sheng-hua DENG Mi-xia FENG Ming-guang |
| author_facet | YING Sheng-hua DENG Mi-xia FENG Ming-guang |
| author_sort | YING Sheng-hua |
| collection | DOAJ |
| description | Contents of hydrophobin-like proteins (HLP) extracted from aerial conidia of 14 isolates of Beauveria bassiana, Paecilomyces fumosoroseus and Metarhizium anisopliae varied significantly among the three fungal species and their isolates. Of those, the maximal HLP content in B. bassiana 2861 conidia was up to 23.4 μg·mg<sup>-1</sup>, 3.7 times greater than the minimal level of M. anisopliae 297 (5.0 μg·mg<sup>-1</sup>). Based on SDS-PAGE analysis, the HLP components differed among the three fungal species with the major molecules of 12.0, 15.0 and 17.5 kDa in 6 B. bassiana isolates and of 15.0 and 17.5 kDa in 5 P. fumosoroseus isolates. However, the HLP components were more variable within M. anisopliae with the molecules of 17.0 and 14.5 kDa in the isolate Ma2125, 19.0 and 13.5 kDa in Ma0201, and only 13.5 kDa in Ma297. Moreover, the aerial conidia, aerial mycelia and submerged mycelia of B. bassiana 2860 isolate grown on Sabouraud dextrose medium with or without agar differed significantly in their HLP contents (26.3, 18.2 and 16.5 μg·mg<sup>-1</sup> respectively), which consistently consisted of the molecules of 15.0 and 17.5 kDa. The inter- and intraspecific variations in HLP contents and components are discussed in association with the understanding of their possible role in ecological adaptation and formulation enhancement. |
| format | Article |
| id | doaj-art-dd57fb128c8c4c6d87426e11d83c1f8f |
| institution | Matheson Library |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2006-07-01 |
| publisher | Zhejiang University Press |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-dd57fb128c8c4c6d87426e11d83c1f8f2025-08-01T05:37:46ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552006-07-013240140510.3785/1008-9209.2006.04.040110089209Hydrophobin-like proteins in aerial conidia of three entomopathogenic hyphmycetes as fungal biocontrol agents: contents and SDS-PAGE profilesYING Sheng-huaDENG Mi-xiaFENG Ming-guangContents of hydrophobin-like proteins (HLP) extracted from aerial conidia of 14 isolates of Beauveria bassiana, Paecilomyces fumosoroseus and Metarhizium anisopliae varied significantly among the three fungal species and their isolates. Of those, the maximal HLP content in B. bassiana 2861 conidia was up to 23.4 μg·mg<sup>-1</sup>, 3.7 times greater than the minimal level of M. anisopliae 297 (5.0 μg·mg<sup>-1</sup>). Based on SDS-PAGE analysis, the HLP components differed among the three fungal species with the major molecules of 12.0, 15.0 and 17.5 kDa in 6 B. bassiana isolates and of 15.0 and 17.5 kDa in 5 P. fumosoroseus isolates. However, the HLP components were more variable within M. anisopliae with the molecules of 17.0 and 14.5 kDa in the isolate Ma2125, 19.0 and 13.5 kDa in Ma0201, and only 13.5 kDa in Ma297. Moreover, the aerial conidia, aerial mycelia and submerged mycelia of B. bassiana 2860 isolate grown on Sabouraud dextrose medium with or without agar differed significantly in their HLP contents (26.3, 18.2 and 16.5 μg·mg<sup>-1</sup> respectively), which consistently consisted of the molecules of 15.0 and 17.5 kDa. The inter- and intraspecific variations in HLP contents and components are discussed in association with the understanding of their possible role in ecological adaptation and formulation enhancement.https://www.academax.com/doi/10.3785/1008-9209.2006.04.0401fungal biocontrol agents<italic>Beauveria bassiana</italic><italic>Metarhizium anisopliae</italic><italic>Paecilomyces fumosoroseus</italic>hydrophobin-like proteins |
| spellingShingle | YING Sheng-hua DENG Mi-xia FENG Ming-guang Hydrophobin-like proteins in aerial conidia of three entomopathogenic hyphmycetes as fungal biocontrol agents: contents and SDS-PAGE profiles 浙江大学学报. 农业与生命科学版 fungal biocontrol agents <italic>Beauveria bassiana</italic> <italic>Metarhizium anisopliae</italic> <italic>Paecilomyces fumosoroseus</italic> hydrophobin-like proteins |
| title | Hydrophobin-like proteins in aerial conidia of three entomopathogenic hyphmycetes as fungal biocontrol agents: contents and SDS-PAGE profiles |
| title_full | Hydrophobin-like proteins in aerial conidia of three entomopathogenic hyphmycetes as fungal biocontrol agents: contents and SDS-PAGE profiles |
| title_fullStr | Hydrophobin-like proteins in aerial conidia of three entomopathogenic hyphmycetes as fungal biocontrol agents: contents and SDS-PAGE profiles |
| title_full_unstemmed | Hydrophobin-like proteins in aerial conidia of three entomopathogenic hyphmycetes as fungal biocontrol agents: contents and SDS-PAGE profiles |
| title_short | Hydrophobin-like proteins in aerial conidia of three entomopathogenic hyphmycetes as fungal biocontrol agents: contents and SDS-PAGE profiles |
| title_sort | hydrophobin like proteins in aerial conidia of three entomopathogenic hyphmycetes as fungal biocontrol agents contents and sds page profiles |
| topic | fungal biocontrol agents <italic>Beauveria bassiana</italic> <italic>Metarhizium anisopliae</italic> <italic>Paecilomyces fumosoroseus</italic> hydrophobin-like proteins |
| url | https://www.academax.com/doi/10.3785/1008-9209.2006.04.0401 |
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