FT-IR study of chilling injury of fishes' cells
CBT cells from colossoma brachypomum and CP cells from eurythermic grass carp were induced with intimidated cold temperature (10℃ and 4℃ respectively) for 0-8 days. Using FT-IR technique, relative contents of the cells were determined, including DNA, RNA, protein, phosphates and glucose. In this way...
Sábháilte in:
| Príomhchruthaitheoirí: | , , , |
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| Formáid: | Alt |
| Teanga: | Béarla |
| Foilsithe / Cruthaithe: |
Zhejiang University Press
2006-07-01
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| Sraith: | 浙江大学学报. 农业与生命科学版 |
| Ábhair: | |
| Rochtain ar líne: | https://www.academax.com/doi/10.3785/1008-9209.2006.04.0428 |
| Clibeanna: |
Cuir clib leis
Níl clibeanna ann, Bí ar an gcéad duine le clib a chur leis an taifead seo!
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| Achoimre: | CBT cells from colossoma brachypomum and CP cells from eurythermic grass carp were induced with intimidated cold temperature (10℃ and 4℃ respectively) for 0-8 days. Using FT-IR technique, relative contents of the cells were determined, including DNA, RNA, protein, phosphates and glucose. In this way, the dynamic information and cell structure in the process of chilling injury can be well obtained. It was found that, for CBT cells, during the 1st-3rd days, the ratios of DNA/amide Ⅱ, RNA/amide Ⅱ, glucose/phosphate and glucose/phospholipids increased; in the 4th day, the four ratios decreased, increased during the 5th-7th days and decreased at the 8th day. The ratio of DNA/RNA decreased in the 1st-3rd days, increased at the 4th day, decreased in the 5th-7th days and increased at the 8th day. For CP cells, the ratios of DNA/amide Ⅱ and RNA/amide Ⅱ did not vary during 1st-3rd days, decreased at the 4th day and increased during the 5th-8th days. The ratios of glucose/phosphate and glucose/phospholipids increased steadily during the 0-2nd days, decreased during the 3rd-7th days, and increased at the 8th day. For RNA/DNA ratio, there are maximum values at the 1st, 4th and 6th days, and minimum values at the 2nd and 8th days. For both kinds of cells, there are good linear relationships between the ratios of RNA/DNA and glucose/phosphate. The variation of those biochemical components is related to the apoptosis process induced by cold temperatures. |
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| ISSN: | 1008-9209 2097-5155 |