Cellular Antioxidant Potential and Cytotoxic Activities of Extracellular Polysaccharides Isolated from <i>Lactobacillus graminis</i> Strain KNUAS018
In the present study, exopolysaccharides (EPS-1, EPS-2, and EPS-3) were extracted from <i>Lactobacillus graminis,</i> and their chemical compositions, bioactivities, and cytotoxicity were comprehensively studied. A higher yield was observed for EPS-1 and EPS-2 with 14.38% and 9.24%, resp...
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MDPI AG
2025-04-01
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| Series: | Polysaccharides |
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| author | Kiseok Han Kumar Vishven Naveen Xin Zhang Anbazhagan Sathiyaseelan Hye-Yong Kim |
| author_facet | Kiseok Han Kumar Vishven Naveen Xin Zhang Anbazhagan Sathiyaseelan Hye-Yong Kim |
| author_sort | Kiseok Han |
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| description | In the present study, exopolysaccharides (EPS-1, EPS-2, and EPS-3) were extracted from <i>Lactobacillus graminis,</i> and their chemical compositions, bioactivities, and cytotoxicity were comprehensively studied. A higher yield was observed for EPS-1 and EPS-2 with 14.38% and 9.24%, respectively. The chemical composition in the samples was studied using FT-IR analysis. The EPS-1 (1 mg/mL) showed higher antioxidant activities with 34.5 ± 6.6% and 93.6 ± 2.3% of DPPH and ABTS radical scavenging, respectively. In the cellular antioxidant assay, the EPS-1 protected oxidative stress-mediated cellular damage in AAPH-treated NIH3T3 cells. In addition, EPS-1 (0.25 mg/mL) treatment augmented the viability of AAPH-stressed RAW264.7 cells (~80%) than AAPH-treated cells (~50%) by reducing the ROS level and associated oxidative damage. Toxicity studies indicated that EPS-1 (1 mg/mL) did not induce notable cytotoxic effects in NIH3T3 cells, RAW264.7 cells, and erythrocytes. Altogether, the findings of this research suggest that <i>L. graminis</i> could be a source of biocompatible polysaccharides with antioxidant properties. |
| format | Article |
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| issn | 2673-4176 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Polysaccharides |
| spelling | doaj-art-dbdfe50ce5be48d28f6a84075ae3343d2025-06-25T14:20:36ZengMDPI AGPolysaccharides2673-41762025-04-01623310.3390/polysaccharides6020033Cellular Antioxidant Potential and Cytotoxic Activities of Extracellular Polysaccharides Isolated from <i>Lactobacillus graminis</i> Strain KNUAS018Kiseok Han0Kumar Vishven Naveen1Xin Zhang2Anbazhagan Sathiyaseelan3Hye-Yong Kim4Department of Bio-Health Convergence, Kangwon National University, Chuncheon 200-701, Republic of KoreaDepartment of Bio-Health Convergence, Kangwon National University, Chuncheon 200-701, Republic of KoreaDepartment of Bio-Health Convergence, Kangwon National University, Chuncheon 200-701, Republic of KoreaDepartment of Bio-Health Convergence, Kangwon National University, Chuncheon 200-701, Republic of KoreaDepartment of Dental Hygiene, College of Health Science, Kangwon National University, Chungcheong 24341, Republic of KoreaIn the present study, exopolysaccharides (EPS-1, EPS-2, and EPS-3) were extracted from <i>Lactobacillus graminis,</i> and their chemical compositions, bioactivities, and cytotoxicity were comprehensively studied. A higher yield was observed for EPS-1 and EPS-2 with 14.38% and 9.24%, respectively. The chemical composition in the samples was studied using FT-IR analysis. The EPS-1 (1 mg/mL) showed higher antioxidant activities with 34.5 ± 6.6% and 93.6 ± 2.3% of DPPH and ABTS radical scavenging, respectively. In the cellular antioxidant assay, the EPS-1 protected oxidative stress-mediated cellular damage in AAPH-treated NIH3T3 cells. In addition, EPS-1 (0.25 mg/mL) treatment augmented the viability of AAPH-stressed RAW264.7 cells (~80%) than AAPH-treated cells (~50%) by reducing the ROS level and associated oxidative damage. Toxicity studies indicated that EPS-1 (1 mg/mL) did not induce notable cytotoxic effects in NIH3T3 cells, RAW264.7 cells, and erythrocytes. Altogether, the findings of this research suggest that <i>L. graminis</i> could be a source of biocompatible polysaccharides with antioxidant properties.https://www.mdpi.com/2673-4176/6/2/33lactic acid bacteriapolysaccharidesantioxidantoxidative stress |
| spellingShingle | Kiseok Han Kumar Vishven Naveen Xin Zhang Anbazhagan Sathiyaseelan Hye-Yong Kim Cellular Antioxidant Potential and Cytotoxic Activities of Extracellular Polysaccharides Isolated from <i>Lactobacillus graminis</i> Strain KNUAS018 Polysaccharides lactic acid bacteria polysaccharides antioxidant oxidative stress |
| title | Cellular Antioxidant Potential and Cytotoxic Activities of Extracellular Polysaccharides Isolated from <i>Lactobacillus graminis</i> Strain KNUAS018 |
| title_full | Cellular Antioxidant Potential and Cytotoxic Activities of Extracellular Polysaccharides Isolated from <i>Lactobacillus graminis</i> Strain KNUAS018 |
| title_fullStr | Cellular Antioxidant Potential and Cytotoxic Activities of Extracellular Polysaccharides Isolated from <i>Lactobacillus graminis</i> Strain KNUAS018 |
| title_full_unstemmed | Cellular Antioxidant Potential and Cytotoxic Activities of Extracellular Polysaccharides Isolated from <i>Lactobacillus graminis</i> Strain KNUAS018 |
| title_short | Cellular Antioxidant Potential and Cytotoxic Activities of Extracellular Polysaccharides Isolated from <i>Lactobacillus graminis</i> Strain KNUAS018 |
| title_sort | cellular antioxidant potential and cytotoxic activities of extracellular polysaccharides isolated from i lactobacillus graminis i strain knuas018 |
| topic | lactic acid bacteria polysaccharides antioxidant oxidative stress |
| url | https://www.mdpi.com/2673-4176/6/2/33 |
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